DNAAF1

Gene Symbol DNAAF1
Entrez Gene 123872
Alt Symbol CILD13, LRRC50, ODA7
Species Human
Gene Type protein-coding
Description dynein, axonemal, assembly factor 1
Other Description dynein assembly factor 1, axonemal|leucine rich repeat containing 50|leucine-rich repeat-containing protein 50|outer row dynein assembly 7 homolog
Swissprots Q96MB6 Q69YJ0 Q8NEP3 Q69YI8 Q69YW5 Q96LP3 B4DJA3
Accessions EAW95500 EAW95501 Q8NEP3 AK057238 BAB71392 AK058059 BAB71645 AK295990 BAG58765 AK310961 AL137334 CAH10706 AL833327 AL833328 CAH10390 AL833336 CAH10394 BC024009 AAH24009 BX648657 DA349893 XM_006721129 XP_006721192 XM_011522850 XP_011521152 XM_011522851 XP_011521153 XM_011522852 XP_011521154 XM_011522853 XP_011521155 XM_011522854 XP_011521156 XM_011522855 XP_011521157 XM_011522856 XP_011521158 XM_011522857 XP_011521159 XM_011522858 XP_011521160 XM_011522859 XP_011521161 XM_011522860 XP_011521162 NM_178452 NP_848547
Function Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms. Involved in regulation of microtubule-based cilia and actin-based brush border microvilli. {ECO:0000269|PubMed:18385425, ECO:0000269|PubMed:19944400, ECO:0000269|PubMed:19944405}.
Subcellular Location Cell projection, cilium {ECO:0000269|PubMed:18385425}. Cytoplasm {ECO:0000269|PubMed:18385425}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:18385425}. Note=In HEK293T cells, it is diffusely cytoplasmic and concentrates at the mitotic spindle poles, while in MDCK cells, it localizes in the cilium. In vivo, this protein is probably restricted to the cilium.
Tissue Specificity Mainly expressed in trachea and testis. {ECO:0000269|PubMed:19944405}.

LRRC50 (D-14) - sc-164917 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LRRC50 (D-14): sc-164917. Western blot analysis of LRRC50 expression in U-698-M whole cell lysate.

LRRC50 (X-22) - sc-133762 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
LRRC50 (X-22): sc-133762. Western blot analysis of LRRC50 expression in 721 B whole cell lysate.

Anti-LRRC50 Antibody (aa151-200) - LS-C82744 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-LRRC50 Antibody (aa108-157) - LS-C111509 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
LRRC50 antibody LS-C111509 Western blot of HepG2 cell lysate.

Anti-LRRC50 Antibody (clone 3E2) - LS-C173857 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.

Anti-LRRC50 Antibody (clone 3A1) - LS-C173856 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.

Anti-LRRC50 Antibody (clone 5F8) - LS-C337696 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5F8
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.

Anti-LRRC50 Antibody (clone 4E4) - LS-C173838 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4E4
Anti-LRRC50 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LRRC50.

Anti-LRRC50 Antibody (clone 3B10) - LS-C173839 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3B10
Western blot of extracts (35 ug) from 9 different cell lines by using g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Anti-LRRC50 Antibody (clone 3F9) - LS-C337698 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F9
Western blot of extracts (35ug) from 9 different cell lines by using anti-LRRC50 monoclonal antibody.

Anti-LRRC50 Antibody (clone 4H3) - LS-C337699 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4H3
Western blot of extracts (35ug) from 9 different cell lines by using anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Anti-LRRC50 Antibody (clone 2C4) - LS-C173842 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C4
Western blot of extracts (35 ug) from 9 different cell lines by using g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

LRRC50 Antibody (2C4) - NBP2-01936 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C4
Western Blot: LRRC50 Antibody (2C4) [NBP2-01936] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

LRRC50 Antibody - NBP1-85005 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: LRRC50 Antibody [NBP1-85005] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles, plasma membrane & cytosol.

LRRC50 Antibody - NBP2-17171 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: LRRC50 Antibody [NBP2-17171] - Sample (50 ug of whole cell lysate) A: mouse testis, 7. 5% SDS PAGE gel, diluted at 1:1000.

Anti-DNAAF1 antibody produced in rabbit - HPA041166 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-LRRC50: <B>Cat. No. HPA041166</B>: Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.

Anti-LRRC50 antibody produced in rabbit - SAB2101390 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-LRRC50: <B>Cat. No.SAB2101390</B>: LRRC50 in HepG2 cell lysate with LRRC50 antibody at 1.0 μg/mL.

LRRC50 antibody - N-terminal region (ARP53358_P050) - ARP53358_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

LRRC50 antibody - N-terminal region (ARP53359_P050) - ARP53359_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DAAF1 Antibody - N-terminal region (ARP75848_P050) - ARP75848_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DAAF1 Antibody - middle region (ARP75849_P050) - ARP75849_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

LRRC50 antibody, N-term - GTX45545 from GeneTex

Prices $319.00
Sizes 50 μg
Host Rabbit
Clonality Polyclonal

LRRC50 antibody, N-term - GTX45546 from GeneTex

Prices $319.00
Sizes 50 μg
Host Rabbit
Clonality Polyclonal

DNAAF1 antibody - 10R-6892 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A1

DNAAF1 antibody - 10R-6893 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E2
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