Gene Symbol | B3GAT3 |
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Entrez Gene | 26229 |
Alt Symbol | GLCATI, glcUAT-I |
Species | Human |
Gene Type | protein-coding |
Description | beta-1,3-glucuronyltransferase 3 |
Other Description | Sqv-8-like protein|UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase|beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)|galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 |
Swissprots | B7ZAB3 Q9UEP0 Q96I06 O94766 |
Accessions | EAW74042 EAW74043 EAW74044 O94766 AB009598 BAA34537 AJ005865 CAA06742 AK075166 AK297970 BAG60280 AK310200 AK316228 BAH14599 BC007906 AAH07906 BC011173 BC019832 AAH19832 BC071961 AAH71961 HQ447823 ADQ32307 XM_011544936 XP_011543238 NM_001288721 NP_001275650 NM_001288722 NP_001275651 NM_001288723 NP_001275652 NM_012200 NP_036332 NR_109991 |
Function | Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP- GlcUA) during completion of linkage region formation. {ECO:0000269|PubMed:24425863}. |
Subcellular Location | Golgi apparatus membrane {ECO:0000269|PubMed:21763480}; Single-pass type II membrane protein {ECO:0000269|PubMed:21763480}. Golgi apparatus, cis-Golgi network {ECO:0000269|PubMed:21763480}. |
Tissue Specificity | Ubiquitous (but weakly expressed in all tissues examined). |
Top Pathways | Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate, Glycosaminoglycan biosynthesis - heparan sulfate / heparin |
GlcAT-I CRISPR/Cas9 KO Plasmid (h) - sc-407780 from Santa Cruz Biotechnology
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GlcAT-I HDR Plasmid (h) - sc-407780-HDR from Santa Cruz Biotechnology
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GlcAT-I Double Nickase Plasmid (h) - sc-407780-NIC from Santa Cruz Biotechnology
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GlcAT-I Double Nickase Plasmid (h2) - sc-407780-NIC-2 from Santa Cruz Biotechnology
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GlcAT-I CRISPR Activation Plasmid (h) - sc-407780-ACT from Santa Cruz Biotechnology
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GlcAT-I CRISPR Activation Plasmid (h2) - sc-407780-ACT-2 from Santa Cruz Biotechnology
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GlcAT-I Lentiviral Activation Particles (h) - sc-407780-LAC from Santa Cruz Biotechnology
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GlcAT-I Lentiviral Activation Particles (h2) - sc-407780-LAC-2 from Santa Cruz Biotechnology
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GlcAT-I siRNA (h) - sc-96989 from Santa Cruz Biotechnology
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GlcAT-I shRNA Plasmid (h) - sc-96989-SH from Santa Cruz Biotechnology
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GlcAT-I shRNA (h) Lentiviral Particles - sc-96989-V from Santa Cruz Biotechnology
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beta-1,3-Glucuronyltransferase 3/B3GAT3 RNAi - H00026229-R01 from Novus Biologicals
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MISSION® esiRNA - EHU044411 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3804 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3804 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR03659 from Sigma-Aldrich
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B3GAT3 siRNA (Human) - CRH8765 from Cohesion Biosciences
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B3GAT3 - MBS8202953 from MyBioSource
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Human B3GAT3 siRNA - orb269267 from Biorbyt
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B3GAT3 shRNA Plasmid (Human) - CHH8765 from Cohesion Biosciences
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B3GAT3 miRNA 3'UTR clone - MiUTR3H-08405 from Creative biogene
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