| Gene Symbol | NLK |
|---|---|
| Entrez Gene | 51701 |
| Alt Symbol | - |
| Species | Human |
| Gene Type | protein-coding |
| Description | nemo-like kinase |
| Other Description | serine/threonine-protein kinase NLK |
| Swissprots | Q2PNI9 Q6P2A3 B2RCX1 Q9UBE8 |
| Accessions | ABC40748 EAW51060 EAW51061 EAW51062 EAW51063 Q9UBE8 AF180819 AAD56013 AF197898 AAF04857 AK024686 AK315315 BAG37718 AL036279 AL161970 BC064663 AAH64663 BM723604 BQ636970 DA224552 DA573077 U69562 XM_005257988 XP_005258045 XM_011524888 XP_011523190 XR_934482 NM_016231 NP_057315 |
| Function | Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin tar |
| Subcellular Location | Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Note=Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity). {ECO:0000250}. |
| Top Pathways | Wnt signaling pathway, Adherens junction, MAPK signaling pathway |
Nlk CRISPR/Cas9 KO Plasmid (h) - sc-401672 from Santa Cruz Biotechnology
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Nlk HDR Plasmid (h) - sc-401672-HDR from Santa Cruz Biotechnology
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Nlk Double Nickase Plasmid (h) - sc-401672-NIC from Santa Cruz Biotechnology
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Nlk Double Nickase Plasmid (h2) - sc-401672-NIC-2 from Santa Cruz Biotechnology
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Nlk CRISPR Activation Plasmid (h) - sc-401672-ACT from Santa Cruz Biotechnology
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Nlk CRISPR Activation Plasmid (h2) - sc-401672-ACT-2 from Santa Cruz Biotechnology
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Nlk Lentiviral Activation Particles (h) - sc-401672-LAC from Santa Cruz Biotechnology
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Nlk Lentiviral Activation Particles (h2) - sc-401672-LAC-2 from Santa Cruz Biotechnology
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Nlk siRNA (h) - sc-36079 from Santa Cruz Biotechnology
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Nlk shRNA Plasmid (h) - sc-36079-SH from Santa Cruz Biotechnology
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Nlk shRNA (h) Lentiviral Particles - sc-36079-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU046391 from Sigma-Aldrich
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MISSION® siRNA - SIHK1417 from Sigma-Aldrich
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MISSION® siRNA - SIHK1419 from Sigma-Aldrich
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MISSION® siRNA - SIHK1418 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1577 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1577 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR10355 from Sigma-Aldrich
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NLK siRNA (Human) - CRH9907 from Cohesion Biosciences
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shRNA set against Human NLK (NM_016231.4) - SHH352836 from Creative biogene
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shRNA set against Human NLK(NM_016231.4) - SHR012640 from Creative biogene
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NLK shRNA Plasmid (Human) - CHH9907 from Cohesion Biosciences
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NLK miRNA 3'UTR clone - MiUTR1H-06791 from Creative biogene
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