PIM1

Gene Symbol PIM1
Entrez Gene 5292
Alt Symbol PIM
Species Human
Gene Type protein-coding
Description Pim-1 proto-oncogene, serine/threonine kinase
Other Description Oncogene PIM1|pim-1 kinase 44 kDa isoform|pim-1 oncogene (proviral integration site 1)|proto-oncogene serine/threonine-protein kinase pim-1|serine/threonine-protein kinase pim-1
Swissprots Q38RT9 Q96RG3 P11309 Q5T7H7
Accessions AAA60090 AAK70871 EAX03934 EAX03935 P11309 AK301158 BAG62748 AK301314 BAG62865 AK309347 BC020224 AAH20224 DA434260 DA481890 DA489942 DQ022562 AAY87461 M16750 AAA60089 M24779 AAA81553 M54915 AAA36447 NM_001243186 NP_001230115 NM_002648 NP_002639
Function Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl- X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis.
Subcellular Location Isoform 2: Cytoplasm. Nucleus.
Tissue Specificity Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines. {ECO:0000269|PubMed:16186805}.
Top Pathways Jak-STAT signaling pathway, Acute myeloid leukemia, MicroRNAs in cancer

Pim-1 Antibody - 2907 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from K562, Raji and NK-92 cell lines using Pim-1 Antibody.

Pim-1 (C93F2) Rabbit mAb - 3247 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C93F2
Western blot analysis of extracts from various cell lines using Pim-1 (C93F2) Rabbit mAb.

Phospho-Pim-1 (Tyr218) Antibody - 4721 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Raji cells, untreated or treated with anti-CD40 (30 min), using Phospho-Pim-1 (Tyr218) Antibody.

Pim-1 Antibody - 4722 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Pim-1 (G-11) - sc-374116 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pim-1 (G-11): sc-374116. Western blot analysis of Pim-1 expression in mouse spleen tissue extract (A) and K-562 whole cell lysate (B).

Pim-1 (C-20) - sc-7856 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Pim-1 (E-16) - sc-26375 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pim-1 (E-16): sc-26375. Western blot analysis of Pim-1 expression in K-562 (A) and PC-3 (B) whole cell lysates.

Pim-1 (12H8) - sc-13513 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pim-1 (12H8): sc-13513. Western blot analysis of Pim-1 expression in K-562 whole cell lysate.

Pim-1 (H-43) - sc-28777 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Pim-1 (H-43): sc-28777. Western blot analysis of Pim-1 expression in mouse spleen tissue extract.

Pim-1 (N-16) - sc-7857 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pim-1 (N-16): sc-7857. Western blot analysis of Pim-1 expression in mouse spleen tissue extract.

Anti-PIM1 (phospho Y309) antibody - ab74047 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PIM1 (phospho Y309) antibody (ab74047) at 1/500 dilutionLane 1 : HUVEC cell extracts treated with PMA (125ng/ml, 30mins)Lane 2 : HUVEC cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-PIM1 antibody - ab85898 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-PIM1 antibody - ab74118 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PIM1 antibody (ab74118) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from Jurkat cells, plus peptide at 10 µgLysates/proteins at 5 µg per lane.

Anti-PIM1 antibody - ab54457 from Abcam

Prices $359.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PIM1 antibody (ab54457) at 1/1000 dilutionLane 1 : K562 whole cell lysate at 50 µgLane 2 : Immunoprecipitate of K562 whole cell lysate (4 ug/mg protein)

Anti-PIM1 antibody [2C9] - ab117518 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2C9
ab117518, at 5 µg/ml, staining PIM1 in Formalin-fixed, Paraffin-embedded Human adrenal tissue by Immunohistochemistry.

Anti-PIM1 antibody [3F5] - ab189154 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3F5
Immunohistochemical analysis of formalin fixed, paraffin embedded Human adrenal tissue labeling PIM1 with ab189154 at 5 µg/ml.

Anti-PIM1 antibody [6A2] - ab117525 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 6A2
ab117525, at 5 µg/ml, staining PIM1 in human skeletal muscle tissue by immunohistochemistry.

Anti-PIM1 antibody [EP2645Y] - ab75776 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2645Y
Anti-PIM1 antibody [EP2645Y] (ab75776) at 1/20000 dilution + Jurkat at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-PIM1 antibody [EP2645Y] (Alexa Fluor® 488) - ab200748 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2645Y
ab200748 staining PIM1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200748 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min).

Anti-PIM1 antibody [EP2645Y] (Alexa Fluor® 647) - ab200749 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2645Y
ab200749 staining PIM1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200749 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min).

Anti-PIM1 / Pim-1 Antibody (C-Terminus) - LS-C90263 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PIM1 / Pim-1 Antibody (N-Terminus) - LS-C107526 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PIM1 antibody LS-C107526 Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.

Anti-PIM1 / Pim-1 Antibody - LS-C146955 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PIM1 / Pim-1 Antibody (N-Terminus) - LS-C111907 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PIM1 antibody LS-C111907 Western blot of 293T cell lysate.

Anti-PIM1 / Pim-1 Antibody (aa281-330) - LS-C117770 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from Jurkat cells, using Pim-1 (Ab-309) Antibody. The lane on the right is treated with the synthesized peptide.
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