| Gene Symbol | EIF4A3 |
|---|---|
| Entrez Gene | 9775 |
| Alt Symbol | DDX48, MUK34, NMP265, NUK34, RCPS, eIF4AIII |
| Species | Human |
| Gene Type | protein-coding |
| Description | eukaryotic translation initiation factor 4A3 |
| Other Description | ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD (Asp-Glu-Ala-Asp) box polypeptide 48|DEAD box protein 48|NMP 265|eIF-4A-III|eIF4A-III|eukaryotic initiation factor 4A-III|eukaryotic initiation factor 4A-like NUK-34|eukaryotic translation initiation factor 4A|hNMP 265|nuclear matrix protein 265 |
| Swissprots | P38919 Q15033 Q6IBQ2 Q96A18 |
| Accessions | EAW89584 EAW89585 P38919 AK290608 BAF83297 BC003662 AAH03662 BC004386 AAH04386 BC011151 AAH11151 CA428254 CR456750 CAG33031 CR749455 D21853 BAA04879 DA667983 X79538 CAA56074 XM_011525522 XP_011523824 NM_014740 NP_055555 |
| Function | ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by |
| Subcellular Location | Nucleus. Nucleus speckle. Cytoplasm. Note=Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites (By similarity). {ECO:0000250}. |
| Tissue Specificity | Ubiquitously expressed. {ECO:0000269|PubMed:10623621}. |
| Top Pathways | Spliceosome, RNA transport, mRNA surveillance pathway |
eIF4AIII CRISPR/Cas9 KO Plasmid (h) - sc-402660 from Santa Cruz Biotechnology
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eIF4AIII HDR Plasmid (h) - sc-402660-HDR from Santa Cruz Biotechnology
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eIF4AIII Double Nickase Plasmid (h) - sc-402660-NIC from Santa Cruz Biotechnology
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eIF4AIII Double Nickase Plasmid (h2) - sc-402660-NIC-2 from Santa Cruz Biotechnology
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eIF4AIII CRISPR Activation Plasmid (h) - sc-402660-ACT from Santa Cruz Biotechnology
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eIF4AIII CRISPR Activation Plasmid (h2) - sc-402660-ACT-2 from Santa Cruz Biotechnology
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eIF4AIII Lentiviral Activation Particles (h) - sc-402660-LAC from Santa Cruz Biotechnology
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eIF4AIII Lentiviral Activation Particles (h2) - sc-402660-LAC-2 from Santa Cruz Biotechnology
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eIF4AIII siRNA (h) - sc-44528 from Santa Cruz Biotechnology
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eIF4AIII shRNA Plasmid (h) - sc-44528-SH from Santa Cruz Biotechnology
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eIF4AIII shRNA (h) Lentiviral Particles - sc-44528-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU025941 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN7773 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND7773 from Sigma-Aldrich
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EIF4A3 siRNA (Human) - CRH6537 from Cohesion Biosciences
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EIF4A3 RNAi - H00009775-R02 from Novus Biologicals
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EIF4A3 - MBS8225680 from MyBioSource
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shRNA set against Human EIF4A3 (NM_014740.3) - SHH284613 from Creative biogene
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Human EIF4A3 siRNA - orb267039 from Biorbyt
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EIF4A3 shRNA Plasmid (Human) - CHH6537 from Cohesion Biosciences
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EIF4A3 miRNA 3'UTR clone - MiUTR3H-10083 from Creative biogene
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