Gene Symbol | USP44 |
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Entrez Gene | 84101 |
Alt Symbol | - |
Species | Human |
Gene Type | protein-coding |
Description | ubiquitin specific peptidase 44 |
Other Description | deubiquitinating enzyme 44|ubiquitin carboxyl-terminal hydrolase 44|ubiquitin specific protease 44|ubiquitin thioesterase 44|ubiquitin thiolesterase 44|ubiquitin-specific-processing protease 44 |
Swissprots | Q9H0E7 B2RDW3 |
Accessions | EAW97540 EAW97541 EAW97542 Q9H0E7 AA101229 AK027434 AK315697 BAG38060 AL136825 CAB66759 AM392541 CAL37419 BC030704 AAH30704 BI458616 BM023146 DA092275 DB096497 DB443014 DB454726 DB456039 DB462518 DQ890845 ABM81771 DQ893999 ABM84925 EU832278 ACE87535 EU832363 ACE86848 HY021046 XM_005269172 XP_005269229 XM_005269173 XP_005269230 XM_005269174 XP_005269231 XM_006719628 XP_006719691 XM_011538800 XP_011537102 XM_011538801 XP_011537103 XM_011538802 XP_011537104 XM_011538803 XP_011537105 XM_011538804 XP_011537106 XM_011538805 XP_011537107 XM_011538806 XP_011537108 XR_944757 XR_944758 NM_001042403 NP_001035862 NM_001278393 NP_001265322 NM_032147 NP_115523 NR_125360 |
Function | Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination. {ECO:0000269|PubMed:17443180, ECO:0000269|PubMed:22681888}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:20402667}. Note=Peaks in interphase, with relatively low levels maintained throughout mitosis. {ECO:0000250}. |
Tissue Specificity | Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia. {ECO:0000269|PubMed:14715245, ECO:0000269|PubMed:21853124}. |
USP44 CRISPR/Cas9 KO Plasmid (h) - sc-403084 from Santa Cruz Biotechnology
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USP44 HDR Plasmid (h) - sc-403084-HDR from Santa Cruz Biotechnology
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USP44 Double Nickase Plasmid (h) - sc-403084-NIC from Santa Cruz Biotechnology
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USP44 Double Nickase Plasmid (h2) - sc-403084-NIC-2 from Santa Cruz Biotechnology
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USP44 CRISPR Activation Plasmid (h) - sc-403084-ACT from Santa Cruz Biotechnology
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USP44 CRISPR Activation Plasmid (h2) - sc-403084-ACT-2 from Santa Cruz Biotechnology
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USP44 Lentiviral Activation Particles (h) - sc-403084-LAC from Santa Cruz Biotechnology
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USP44 Lentiviral Activation Particles (h2) - sc-403084-LAC-2 from Santa Cruz Biotechnology
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USP44 siRNA (h) - sc-76857 from Santa Cruz Biotechnology
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USP44 shRNA Plasmid (h) - sc-76857-SH from Santa Cruz Biotechnology
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USP44 shRNA (h) Lentiviral Particles - sc-76857-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU005411 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22593 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22593 from Sigma-Aldrich
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USP44 siRNA (Human) - CRJ3299 from Cohesion Biosciences
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USP44 - MBS8227101 from MyBioSource
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shRNA set against Human USP44 (NM_032147.2) - SHH441640 from Creative biogene
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shRNA set against Human USP44(NM_001042403.1) - SHL118556 from Creative biogene
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USP44 shRNA Plasmid (Human) - CHJ3299 from Cohesion Biosciences
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USP44 miRNA 3'UTR clone - MiUTR3H-11597 from Creative biogene
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USP44 miRNA 3'UTR clone - MiUTR3H-11598 from Creative biogene
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