| Gene Symbol | KLHL20 |
|---|---|
| Entrez Gene | 27252 |
| Alt Symbol | KHLHX, KLEIP, KLHLX |
| Species | Human |
| Gene Type | protein-coding |
| Description | kelch-like family member 20 |
| Other Description | Kelch motif containing protein|kelch-like 20|kelch-like ECT2 interacting protein|kelch-like ECT2-interacting protein|kelch-like protein 20|kelch-like protein X |
| Swissprots | Q5TZF2 Q9Y2M5 Q9H457 B4DUR0 B3KMA0 Q5ZF45 |
| Accessions | EAW90952 EAW90953 EAW90954 EAW90955 Q9Y2M5 AB026190 BAA77027 AJ844466 CAH59617 AK001430 BAG50912 AK300755 BAG62422 AK308048 BC005253 AAH05253 BC063418 AAH63418 CB143306 XM_005245093 XP_005245150 XM_011509420 XP_011507722 XR_921766 XR_921767 NM_014458 NP_055273 |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis (PubMed:20389280). The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'- linked ubiquitination (PubMed:24768539). Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking (PubMed:24768539). Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (PubM |
| Subcellular Location | Cytoplasm, perinuclear region. Nucleus. Golgi apparatus, trans-Golgi network. Cell projection, axon {ECO:0000250}. Cell projection, dendrite {ECO:0000250}. Note=Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination (PubMed:20389280). {ECO:0000269|PubMed:20389280}. |
KLHL20 CRISPR/Cas9 KO Plasmid (h) - sc-411984 from Santa Cruz Biotechnology
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KLHL20 HDR Plasmid (h) - sc-411984-HDR from Santa Cruz Biotechnology
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KLHL20 Double Nickase Plasmid (h) - sc-411984-NIC from Santa Cruz Biotechnology
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KLHL20 Double Nickase Plasmid (h2) - sc-411984-NIC-2 from Santa Cruz Biotechnology
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KLHL20 CRISPR Activation Plasmid (h) - sc-411984-ACT from Santa Cruz Biotechnology
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KLHL20 CRISPR Activation Plasmid (h2) - sc-411984-ACT-2 from Santa Cruz Biotechnology
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KLHL20 Lentiviral Activation Particles (h) - sc-411984-LAC from Santa Cruz Biotechnology
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KLHL20 Lentiviral Activation Particles (h2) - sc-411984-LAC-2 from Santa Cruz Biotechnology
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KLHL20 siRNA (h) - sc-88301 from Santa Cruz Biotechnology
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KLHL20 shRNA Plasmid (h) - sc-88301-SH from Santa Cruz Biotechnology
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KLHL20 shRNA (h) Lentiviral Particles - sc-88301-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU079591 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN11279 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND11279 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR09702 from Sigma-Aldrich
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KLHL20 siRNA (Human) - CRH9044 from Cohesion Biosciences
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shRNA set against Human KLHL20 (NM_014458.3) - SHH326147 from Creative biogene
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shRNA set against Human KLHL20(NM_014458.3) - SHL201204 from Creative biogene
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Human KLHL20 siRNA - orb269546 from Biorbyt
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KLHL20 shRNA Plasmid (Human) - CHH9044 from Cohesion Biosciences
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KLHL20 miRNA 3'UTR clone - MiUTR1H-05371 from Creative biogene
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