ASAP1

Gene Symbol ASAP1
Entrez Gene 50807
Alt Symbol AMAP1, CENTB4, DDEF1, PAG2, PAP, ZG14P
Species Human
Gene Type protein-coding
Description ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
Other Description 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein|130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein|ADP-ribosylation factor-directed GTPase-activating protein 1|ARF GTPase-activating protein 1|DEF-1|PIP2-dependent ARF1 GAP|arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1|centaurin, beta 4|development and differentiation-enhancing factor 1
Swissprots B2RNV3 Q9ULH1
Accessions CBX47524 CCQ43599 EAW92130 EAW92131 Q9ULH1 AB033075 BAA86563 AF222859 AAF67666 AI494058 AI656533 AK056811 AK314695 AW236866 AW236958 BC020631 AAH20631 BC070268 BC105978 BC137135 AAI37136 BC144493 BX537768 CAD97831 DA319233 XM_005250925 XP_005250982 XM_006716563 XP_006716626 XM_006716564 XP_006716627 XM_006716565 XP_006716628 XM_006716566 XP_006716629 XM_006716567 XP_006716630 XM_011517052 XP_011515354 XM_011517053 XP_011515355 NM_001247996 NP_001234925 NM_018482 NP_060952
Function Possesses phosphatidylinositol 4,5-bisphosphate- dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis. {ECO:0000250, ECO:0000269|PubMed:20393563}.
Subcellular Location Cytoplasm {ECO:0000250}. Membrane {ECO:0000250}. Note=Predominantly cytoplasmic. Partially membrane- associated. {ECO:0000250}.
Top Pathways Fc gamma R-mediated phagocytosis, Endocytosis

DDEF1 (B-10) - sc-374410 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DDEF1 (B-10): sc-374410. Western blot analysis of DDEF1 expression in IMR-32 whole cell lysate.

DDEF1 (C-18) - sc-66365 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

DDEF1 (E-12) - sc-374411 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DDEF1 (E-12): sc-374411. Western blot analysis of DDEF1 expression in IMR-32 whole cell lysate.

DDEF1 (E-19) - sc-66366 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DDEF1 (E-19): sc-66366. Western blot analysis of DDEF1 expression in IMR-32 whole cell lysate.

DDEF1 (19) - sc-136392 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DDEF1 (19): sc-136392. Western blot analysis of DDEF1 expression in mouse brain tissue extract.

DDEF1 (P7Q) - sc-81896 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
DDEF1 (P7Q): sc-81896. Western blot analysis of DDEF1 expression in IMR-32 whole cell lysate.

DDEF1 (P-20) - sc-66367 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DDEF1 (P-20): sc-66367. Western blot analysis of DDEF1 expression in IMR-32 whole cell lysate.

Anti-ASAP1 / DDEF1 antibody - ab12423 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP1 / DDEF1 antibody - ab76916 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-ASAP1 / DDEF1 antibody (ab76916) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-ASAP1 / DDEF1 antibody - ab54747 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-ASAP1 / DDEF1 antibody [7B12] - ab125729 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 7B12

Anti-ASAP1 Antibody (aa777-787) - LS-C19043 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ASAP1 Antibody - Immunofluorescent Microscopy. Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.

Anti-ASAP1 Antibody (aa1030-1130, clone 10C3, Azide-free) - LS-C183495 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10C3

Anti-ASAP1 Antibody (aa360-388, AP) - LS-C251826 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP1 Antibody (aa360-388, APC) - LS-C251827 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP1 Antibody (aa360-388, Biotin) - LS-C251828 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP1 Antibody (clone 1B5) - LS-C139195 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1B5
DDEF1 monoclonal antibody (M02), clone 1B5. Western blot of DDEF1 expression in NIH/3T3.

Anti-ASAP1 Antibody (aa360-388, Azide-free, HRP) - LS-C251830 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP1 Antibody (aa360-388, FITC) - LS-C251829 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP1 Antibody (aa360-388, PE) - LS-C251831 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP1 Antibody (aa360-388) - LS-C156528 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ASAP1 Antibody western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The ASAP1 antibody detected the ASAP1 protein (arrow).

Anti-ASAP1 Antibody (aa962-978) - LS-C148543 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of ASAP1 in mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148543 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Anti-ASAP1 Antibody (aa700-750) - LS-C288874 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP1 Antibody (aa750-800) - LS-C288876 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP1 Antibody (aa359-388) - LS-C319625 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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