Non-alcoholic fatty liver disease (NAFLD)



PathScan ® Phospho-Akt (Thr308) Sandwich ELISA Antibody Pair - 7144 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan ® Phospho-Akt (Thr308) Sandwich ELISA Antibody Pair #7144 is shown. After overnight starvation, NIH/3T3 cells were treated with PDGF (50 ng/ml) for 10 minutes at 37ºC and then lysed.

PathScan ® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit - 7134 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Relationship between protein concentration of lysates from untreated and PDGF-treated NIH/3T3 cells and immediate light generation with chemiluminescent substrate is shown. Cells (80% confluence) were treated with PDGF #9909 (50 ng/ml) and lysed after incubation at 37ºC for 20 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Phospho-Akt (Thr308) Chemiluminescent Sandwich ELISA Kit - 7135 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Relationship between protein concentration of lysates from untreated and PDGF-treated NIH/3T3 cells and immediate light generation with chemiluminescent substrate is shown. Cells (80% confluence) were treated with PDGF #9909 (50 ng/ml) and lysed after incubation at 37ºC for 5 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Antibody Pair - 7143 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan Phospho-Akt1 (Ser473) Sandwich ELISA Antibody Pair #7143 is shown. After overnight starvation, NIH/3T3 cells were treated with PDGF (50 ng/ml) for 20 minutes at 37ºC and then lysed.

PathScan ® Phospho-Akt (Thr308) Sandwich ELISA Kit - 7252 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of NIH/3T3 cells with PDGF stimulates phosphorylation of Akt at Thr308, detected by PathScan® Phospho-Akt (Thr308) Sandwich ELISA Kit #7252, but does not affect the level of total Akt detected by PathScan® Total Akt1 Sandwich ELISA Kit #7170. OD 450 nm readings are shown in the top figure, while the corresponding Western blots using Phospho-Akt (Thr308) Antibody #9275 (right panel) or Akt Antibody #9272 (left panel), are shown in the bottom figure.

PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Kit - 7160 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Phospho-Akt1 (Ser473) is detected in untreated Jurkat cells, where as no signal is detected with treatment with LY294002 , a specific PI3 Kinase inhibitor using PathScan ® Phospho-Akt1 (Ser473) Sandwich ELISA Kit #7160.

PathScan ® Total Akt1 Sandwich ELISA Antibody Pair - 7142 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan ® Total Akt1 Sandwich ELISA Antibody Pair #7142. After overnight starvation, NIH/3T3 cells were treated with PDGF (50 ng/ml) for 10 minutes at 37ºC and then lysed.

PathScan ® Total Akt1 Chemiluminescent Sandwich ELISA Kit - 7132 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Relationship between protein concentration of lysates from untreated and PDGF-treated NIH/3T3 cells and immediate light generation with chemiluminescent substrate is shown. Cells (80% confluence) were treated with PDGF #9909 (50 ng/ml) and lysed after incubation at 37ºC for 20 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Total Akt1 Sandwich ELISA Kit - 7170 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of NIH/3T3 cells with various concentrations of LY294002 blocks PDGF-stimulated phosphorylation of Akt1 at serine 473 detected by PathScan Phospho-Akt1 (Ser473) Sandwich ELISA kit , #7160, but does not affect the level of total Akt1 protein detected by PathScan Total Akt1 Sandwich ELISA kit #7170. OD450 readings are shown in the top figure, while the corresponding Western blot, using Phospho-Akt (Ser473) Antibody #9271 or Akt Antibody #9272, is shown in the bottom figure.

PathScan ® Akt Signaling Antibody Array Kit (Fluorescent Readout) - 9700 from Cell Signaling Technology

Category Antibody Array
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Akt Signaling Antibody Array Kit (Chemiluminescent Readout) - 9474 from Cell Signaling Technology

Category Antibody Array
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® EGFR Signaling Antibody Array Kit (Fluorescent Readout) - 12785 from Cell Signaling Technology

Category Antibody Array
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Immune Cell Signaling Antibody Array Kit (Chemiluminescent Readout) - 13792 from Cell Signaling Technology

Category Antibody Array
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Stress and Apoptosis Signaling Antibody Array Kit (Chemiluminescent Readout) - 12856 from Cell Signaling Technology

Category Antibody Array
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Immune Cell Signaling Antibody Array Kit (Fluorescent Readout) - 13788 from Cell Signaling Technology

Category Antibody Array
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Intracellular Signaling Array Kit (Fluorescent Readout) - 7744 from Cell Signaling Technology

Category ELISA Kit
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Stress and Apoptosis Signaling Antibody Array Kit (Fluorescent Readout) - 12923 from Cell Signaling Technology

Category Antibody Array
Prices $649.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® EGFR Signaling Antibody Array Kit (Chemiluminescent Readout) - 12622 from Cell Signaling Technology

Category Antibody Array
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Intracellular Signaling Array Kit (Chemiluminescent Readout) - 7323 from Cell Signaling Technology

Category ELISA Kit
Prices $609.00
Sizes 1 kit (32 multiplexed assays)

PathScan ® Cell Growth 4-Plex Array Kit - 7136 from Cell Signaling Technology

Category ELISA Kit

PathScan ® Signaling Nodes I 4-Plex Array Kit - 7137 from Cell Signaling Technology

Category ELISA Kit

PathScan ® Inflammation 4-Plex Array Kit - 7138 from Cell Signaling Technology

Category ELISA Kit

PathScan ® Signaling Nodes II 4-Plex Array Kit - 7139 from Cell Signaling Technology

Category ELISA Kit

Akt Kinase Assay Kit (Nonradioactive) - 9840 from Cell Signaling Technology

Category Assay Kit
Prices $379.00
Sizes 40 assays
AKT Kinase activity of PDGF-treated NIH/3T3 cell extracts was analyzed by IP/Kinase assay. Cell extracts (200 μl) were incubated overnight with Immobilized Phospho-Akt (Ser473) (D9E) Rabbit mAb #4070. After extensive washing the kinase reaction was performed in the presence of 200 μM of cold ATP and 1 μg of GSK-substrate. Phosphorylation of GSK-3 was measured by Western blot using Phospho-GSK-3 a/β (Ser21/9) Antibody #9327.

HTScan® Akt1 Kinase Assay Kit - 7501 from Cell Signaling Technology

Category Assay Kit
Figure 1. Akt1 kinase activity was measured in a radiometric assay using the following reaction conditions: 5 mM MOPS, pH 7.2, 2.5 mM β-glycerophosphate, 1 mM EGTA, 0.4 mM EDTA, 5 mM MgCl2, 0.05 mM DTT, 50 μM ATP, Substrate: Akt-substrate peptide 200 ng/μL, and variable amount of recombinant Akt1.
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