SF3A1

Gene Symbol	SF3A1
Entrez Gene	10291
Alt Symbol	PRP21, PRPF21, SAP114, SF3A120
Species	Human
Gene Type	protein-coding
Description	splicing factor 3a, subunit 1, 120kDa
Other Description	SAP 114\|pre-mRNA processing 21\|pre-mRNA splicing factor SF3a (120 kDa subunit)\|spliceosome-associated protein 114\|splicing factor 3 subunit 1\|splicing factor 3A subunit 1\|splicing factor 3a, subunit 1, 120kD
Swissprots	E9PAW1 Q15459
Accessions	AAC23435 EAW59878 EAW59879 Q15459 AI681052 AK098463 AK303273 BAG64353 AU100212 AV750569 BC001976 AAH01976 BC007684 AAH07684 BF965438 BI827130 BQ212479 BQ223575 BQ437843 BQ447452 CA440158 CK300534 CR456575 CAG30461 CU013174 CAK54605 CU013462 CAK54904 DB445415 DQ892687 ABM83613 DQ895931 ABM86857 X85237 CAA59494 NM_005877 NP_005868 NM_001005409
Function	Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Subcellular Location	Nucleus {ECO:0000250}.
Tissue Specificity	Ubiquitously expressed.
Top Pathways	Spliceosome

SAP 114 (H-111) - sc-292175 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

SAP 114 (H-111): sc-292175. Western blot analysis of SAP 114 expression in K-562 (A) and Jurkat (B) whole cell lysates.

SAP 114 (N-20) - sc-68784 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

SAP 114 (N-20): sc-68784. Western blot analysis of SAP 114 expression in HeLa (A), K-562 (B), Hep G2 (C), PC-12 (D) and NIH/3T3 (E) whole cell lysates and mouse brain tissue extract (F).

SAP 114 (V-20) - sc-68785 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

SAP 114 (Z-22) - sc-133982 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

SAP 114 (Z-22): sc-133982. Western blot analysis of SAP 114 expression in HeLa (A) and K-562 (B) whole cell lysates.

Anti-SF3A1 antibody - ab69903 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SF3A1 with ab69903 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.

Anti-SF3A1 antibody - ab84698 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ab84698, at 1/250 dilution, staining SF3A1 in formalin-fixed, paraffin-embedded human pancreatic islet cell tumor by Immunohistochemistry, using DAB staining.

Anti-SF3A1 antibody [2C4] - ab139271 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2C4

All lanes : Anti-SF3A1 antibody [2C4] (ab139271) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SF3A1 Lysates/proteins at 5 µg per lane.

Anti-SF3A1 antibody [EPR7666] - ab128868 from Abcam

Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7666

ab128868, at a 1/50 dilution, staining SF3A1 in paraffin embedded Human breast tissue by Immunohistochemistry.

Anti-SF3A1 antibody [EPR7667] - ab128898 from Abcam

Prices	$313.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7667

All lanes : Anti-SF3A1 antibody [EPR7667] (ab128898) at 1/1000 dilutionLane 1 : Human lymph node lysateLane 2 : HeLa lysateLane 3 : K562 lysateLane 4 : Raji lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SF3A1 / SF3A120 Antibody (clone 4D8) - LS-C175400 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	4D8

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SF3A1 mouse monoclonal antibody.

Anti-SF3A1 / SF3A120 Antibody (clone 2C4) - LS-C175399 from LifeSpan Bioscience

Prices	$350.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2C4

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SF3A1 mouse monoclonal antibody.

Anti-SF3A1 / SF3A120 Antibody (aa249-568, clone 3F4) - LS-C336999 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3F4

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-SF3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-SF3A1 / SF3A120 Antibody (Internal, clone EPR7667) - LS-C154440 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7667

Western blot analysis on (A) lymph node, (B) HeLa, (C) K562, and (D) Raji lysates using anti-SF3A1 antibody.

Anti-SF3A1 / SF3A120 Antibody (clone 1B5) - LS-C175336 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1B5

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SF3A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SF3A1.

Anti-SF3A1 / SF3A120 Antibody (clone 1F10) - LS-C175308 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1F10

Anti-SF3A1 / SF3A120 Antibody (Internal, clone EPR7666) - LS-C152538 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7666

IHC of paraffin-embedded human breast tissue using anti-SF3A1 antibody.

Anti-SF3A1 / SF3A120 Antibody (clone 7G6) - LS-C175375 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	7G6

Anti-SF3A1 / SF3A120 Antibody (aa743-793) - LS-C288394 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SF3A1 / SF3A120 Antibody (aa300-350) - LS-C288393 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human and Mouse SF3a120/SAP114 by Immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-SF3a120/SAP114 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-SF3A1 / SF3A120 Antibody (aa350-400) - LS-C288401 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SF3A1 / SF3A120 Antibody (aa500-550) - LS-C286073 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human SF3a120/SAP114 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SF3a120/SAP114 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-SF3A1 / SF3A120 Antibody (clone 8H1) - LS-C175307 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	8H1

IHC of paraffin-embedded Human breast tissue using anti-SF3A1 mouse monoclonal antibody.

Anti-SF3A1 / SF3A120 Antibody (aa300-350) - LS-C286554 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human SF3a120/SAP114 by IHC-IF. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SF3a120/SAP114 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.

Anti-SF3A1 / SF3A120 Antibody - LS-C333292 from LifeSpan Bioscience

Prices	$305.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using SF3A1 antibody.

Anti-SF3A1 / SF3A120 Antibody (aa121-170) - LS-C80355 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

721_B cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 8%.

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