MAP4K4

Gene Symbol MAP4K4
Entrez Gene 9448
Alt Symbol FLH21957, HEL-S-31, HGK, MEKKK4, NIK
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase kinase kinase kinase 4
Other Description HPK/GCK-like kinase HGK|MAPK/ERK kinase kinase kinase 4|MEK kinase kinase 4|Ste20 group protein kinase HGK|epididymis secretory protein Li 31|hepatocyte progenitor kinase-like/germinal center kinase-like kinase|nck-interacting kinase
Swissprots O75172 Q9NST7 O95819
Accessions AAY14969 AAY14970 AAY24319 EAX01805 EAX01806 EAX01807 EAX01808 EAX01809 EAX01810 O95819 AB013385 BAA33714 AB014587 BAA31662 AF096300 AAD16137 AK000380 BAA91127 AK001272 AK025610 AK074592 AK074900 AK130085 AK295610 BAH12124 AK296396 BAH12341 AK307879 AL137755 CAB70907 AY212247 AAO32626 AY834276 AAV97884 BC010909 BC040077 BC045694 BC051791 BC054038 BC064691 BC065036 BC156089 AAI56090 BC156887 AAI56888 BU595561 BU625485 DB086475 DR002278 EU794634 ACJ13688 XM_005264044 XP_005264101 XM_005264045 XP_005264102 XM_005264046 XP_005264103 XM_005264047 XP_005264104 XM_005264048 XP_005264105 XM_005264049 XP_005264106 XM_005264050 XP_005264107 XM_005264052 XP_005264109 XM_005264053 XP_005264110 XM_005264054 XP_005264111 XM_005264055 XP_005264112 XM_005264056 XP_005264113 XM_005264057 XP_005264114 XM_005264058 XP_005264115 XM_005264059 XP_005264116 XM_005264060 XP_005264117 XM_005264062 XP_005264119 XM_005264063 XP_005264120 XM_005264064 XP_005264121 XM_005264065 XP_005264122 XM_005264066 XP_005264123 XM_005264068 XP_005264125 XM_005264069 XP_005264126 XM_005264071 XP_005264128 XM_006712865 XP_006712928 XM_006712866 XP_006712929 XM_006712867 XP_006712930 XM_006712868 XP_006712931 XM_006712869 XP_006712932 XM_011512201 XP_011510503 XM_011512202 XP_011510504 XM_011512203 XP_011510505 XM_011512204 XP_011510506 XM_011512205 XP_011510507 NM_001242559 NP_001229488 NM_001242560 NP_001229489 NM_004834 NP_004825 NM_145686 NP_663719 NM_145687 NP_663720
Function Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322. {ECO:0000269|PubMed:21690388, ECO:0000269|PubMed:9890973}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:14966141}.
Tissue Specificity Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta. {ECO:0000269|PubMed:9890973}.
Top Pathways MAPK signaling pathway

HGK (D19H10) Rabbit mAb - 5146 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D19H10
Western blot analsysis of extracts from various cell lines using HGK (D19H10) Rabbit mAb.

HGK Antibody - 3485 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using HGK Antibody.

HGK (M-190) - sc-25738 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HGK (M-190): sc-25738. Western blot analysis of HGK expression in rat brain tissue extract.

HGK (A-19) - sc-6960 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HGK (A-19): sc-6960. Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.

HGK (5-12) - sc-100445 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HGK (5-12): sc-100445. Western blot analysis of HGK expression in K-562 whole cell lysate.

HGK (3C7B5) - sc-135813 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HGK (3C7B5): sc-135813. Western blot analysis of HGK expression against truncated Trx-HGK recombinant protein (A) MBP-HGK (aa 300-400) recombinant protein (B) and human HGK (aa 194-436)-hIgFc transfected CHO-K1 whole cell lysate (C).

Anti-MAP4K4 antibody - ab155583 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MAP4K4 antibody (ab155583) at 1/1000 dilution + Raji whole cell lysate at 30 µg

Anti-MAP4K4 antibody - ab90292 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MAP4K4 antibody (ab90292) at 1 µg/ml + Human fetal small intestine lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-MAP4K4 antibody - ab56569 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing Jurkat cells stained with ab56569 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56569, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MAP4K4 antibody - ab69885 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MAP4K4 antibody (ab69885) at 1/500 dilutionLane 1 : Extracts from COLO cells Lane 2 : Extracts from HUVEC cells Lane 3 : Extracts from HUVEC cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-MAP4K4 antibody - ab80418 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MAP4K4 antibody (ab80418) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgdeveloped using the ECL technique

Anti-MAP4K4 antibody - ab99402 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed paraffin-embedded human colon carcinoma using ab99402 at a dilution of 1/250

Anti-MAP4K4 antibody [4H9E7] - ab70424 from Abcam

Host Mouse
Clonality Monoclonal
Clone 4H9E7
All lanes : Anti-MAP4K4 antibody [4H9E7] (ab70424) at 1/500 dilutionLane 1 : Truncated Trx-MAP4K4 recombinant protein Lane 2 : MBP-MAP4K4 (aa400-500) recombinant protein

Anti-MAP4K4 antibody [EPR1642(2)] - ab134092 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1642(2)
All lanes : Anti-MAP4K4 antibody [EPR1642(2)] (ab134092) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Jurkat cell lysateLane 3 : SH-SY5Y cell lysateLysates/proteins at 10 µg/ml per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-MAP4K4 antibody [SP177] - ab150300 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP177
Flow cytometric analysis of rabbit anti-MAP4K4 (SP177) antibody ab150300 (1/400) in HepG2 cells(green) compared to negative control of rabbit IgG (blue).

Anti-MAP4K4 Antibody (clone 4H9E7) - LS-C108875 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4H9E7
Western blot using MAP4K4 mouse monoclonal antibody against truncated Trx-MAP4K4 recombinant protein (1), MBP-MAP4K4 (aa300-400) recombinant protein (2) and MAP4K4(aa194-436)-hIgGFc transfected CH0-K1 cell lysate(3).

Anti-MAP4K4 Antibody (phospho-Ser801) - LS-C96781 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Dot blot of anti-MAP4K4-pS801 Phospho-specific antibody (RB11324) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Anti-MAP4K4 Antibody (phospho-Ser629) - LS-C96768 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Dot blot of anti-MAP4K4-pS629 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Anti-MAP4K4 Antibody (aa618-649) - LS-C99816 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of anti-HGK Antibody in Jurkat cell line lysates (35 ug/lane). HGK(arrow) was detected using the purified antibody.

Anti-MAP4K4 Antibody (Ala553) - LS-C191011 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal

Anti-MAP4K4 Antibody (aa506-534, AP) - LS-C215714 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP4K4 Antibody (aa931-947) IHC-plus™ - LS-B712 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MAP4K4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B712 concentration 20 ug/ml.

Anti-MAP4K4 Antibody (aa506-534, APC) - LS-C215715 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP4K4 Antibody (aa506-534, Biotin) - LS-C215717 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAP4K4 Antibody (aa506-534, FITC) - LS-C215716 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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