RNGTT

Gene Symbol RNGTT
Entrez Gene 8732
Alt Symbol CAP1A, HCE, HCE1, hCAP
Species Human
Gene Type protein-coding
Description RNA guanylyltransferase and 5'-phosphatase
Other Description HCAP1|mRNA-capping enzyme
Swissprots O60351 Q5TCW8 O60942 Q8WUM8 E1P513 O43483 E1P514 O60257
Accessions EAW48566 EAW48567 EAW48568 EAW48569 EAW48570 O60942 AB009022 BAA25894 AB009023 BAA25895 AB009024 BAA25896 AB012142 BAA25198 AB012143 BAA25199 AF025654 AAB91559 AI193199 AK295719 BAG58562 AK299777 BAG61660 AK312407 BAG35320 BC019954 AAH19954 BP256840 BP396964 BX537450 CAD97693 JF432295 ADZ15512 XM_011536219 XP_011534521 XR_942617 NM_001286426 NP_001273355 NM_001286428 NP_001273357 NM_003800 NP_003791
Function Bifunctional mRNA-capping enzyme exhibiting RNA 5'- triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. {ECO:0000269|PubMed:21636784, ECO:0000269|PubMed:9473487, ECO:0000269|PubMed:9512541}.
Subcellular Location Nucleus {ECO:0000250}.
Tissue Specificity Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.
Top Pathways mRNA surveillance pathway

RNGTT (D-3) - sc-377464 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RNGTT (D-3): sc-377464. Western blot analysis of RNGTT expression in Jurkat (A) and IMR-32 (B) nuclear extracts.

RNGTT (H-3) - sc-514128 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RNGTT (H-3): sc-514128. Western blot analysis of RNGTT expression in Jurkat nuclear extract (A) and Hep G2 (B) and MOLT-4 (C) whole cell lysates.

RNGTT (D-13) - sc-138052 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RNGTT (D-13): sc-138052. Western blot analysis of RNGTT expression in IMR-32 (A) and Hep G2 (B) nuclear extracts.

RNGTT (N-13) - sc-138054 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RNGTT (N-13): sc-138054. Western blot analysis of RNGTT expression in SH-SY5Y nuclear extract.

Anti-HCE antibody - ab176728 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labeling HCE using ab176728 at a 1/200 dilution (1µg/ml), using peroxidase substrate for detection.

Anti-HCE antibody - ab27971 from Abcam

Prices $370.00
Sizes 200 µl
Host Sheep
Clonality Polyclonal
All lanes : Anti-HCE antibody (ab27971) at 1/1000 dilutionLane 1 : Hela nuclear extract at 0.5 µlLane 2 : Hela nuclear extract at 1 µlSecondarydonkey anti-sheep peroxidase conjugate at 1/20000 dilution

Anti-RNGTT / HCAP Antibody IHC-plus™ - LS-B8904 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RNGTT / HCAP antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-RNGTT / HCAP Antibody (aa66-115) - LS-C146391 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RNGTT / HCE antibody LS-C146391 Western blot of Mouse Heart lysate. Antibody concentration 1 ug/ml.

Anti-RNGTT / HCAP Antibody (aa350-400) - LS-C289587 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RNGTT by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RNGTT used at a dilution of 1:200 (1 ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate.

Anti-RNGTT / HCAP Antibody - LS-C335668 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using RNGTT antibody.

Anti-RNGTT / HCAP Antibody (aa1-50) - LS-C289586 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RNGTT by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RNGTT used at a dilution of 1:200 (1 ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate.

RNGTT Antibody - NBP1-49972 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RNGTT Antibody [NBP1-49972] - Detection of Human and Mouse RNGTT by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNGTT antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNGTT was also immunoprecipitated by rabbit anti-RNGTT antibody which recognizes a downstream epitope. For blotting immunoprecipitated RNGTT was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

RNGTT Antibody - NBP1-49973 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RNGTT Antibody [NBP1-49973] - Detection of Human and Mouse RNGTT by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNGTT antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNGTT was also immunoprecipitated by rabbit anti-RNGTT antibody which recognizes an upstream epitope. For blotting immunoprecipitated RNGTT was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

RNGTT Antibody - NBP1-83281 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RNGTT Antibody [NBP1-83281] - Analysis in human cell line RH-30.

RNGTT Antibody - NBP1-32711 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RNGTT Antibody [NBP1-32711] - Sample (30 ug of whole cell lysate) A: HeLa B: Hep G2 C: MOLT4 7.5% SDS PAGE, antibody diluted at 1:1000.

HCE Polyclonal Antibody - bs-15425R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RNGTT antibody produced in rabbit - HPA003750 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RNGTT: <B>Cat. No. HPA003750</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human placenta shows strong nuclear positivity in trophoblastic cells and decidual cells.

HCE antibody [N1N3] - GTX105694 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-HCE antibody [N1N3] used in Western blot. GTX105694

HCE antibody - orb73511 from Biorbyt

Prices $723.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal
Western Blot analysis of A:Hela,B:HepG2, C:MOLT4 whole cell lysate using HCE antibody

mRNA capping enzyme antibody - 10R-8632 from Fitzgerald

Prices $295.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone T16-AF3F1

RNGTT Polyclonal Antibody - A6431 from ABclonal

Prices $130.00, $220.00, $380.00
Sizes 50 µl, 100 µl, 200 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using RNGTT antibody.

RNGTT antibody (OAGA00421) - OAGA00421 from Aviva Systems Biology

Prices $340.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

HCE antibody - orb157447 from Biorbyt

Prices $255.00, $340.00
Sizes 100 μg, 200 μg
Host Rabbit
Clonality Polyclonal

HCE antibody - 70R-12921 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

HCE Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-15425R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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