MBTPS1

Gene Symbol MBTPS1
Entrez Gene 8720
Alt Symbol PCSK8, S1P, SKI-1
Species Human
Gene Type protein-coding
Description membrane-bound transcription factor peptidase, site 1
Other Description endopeptidase S1P|membrane-bound transcription factor site-1 protease|proprotein convertase subtilisin/kexin type 8|site-1 protease|subtilisin/kexin isozyme-1
Swissprots Q14703 Q24JQ2 Q9UF67 A8K6V8
Accessions CCQ43234 EAW95505 EAW95506 Q14703 AK054744 AK091212 BAG52308 AK291773 BAF84462 AK299067 BAG61133 AL133583 CAB63727 AL701753 BC026330 BC114555 AAI14556 BC114961 AAI14962 BM048055 BM680126 BP364386 BX472644 D42053 BAA07653 NM_003791 NP_003782 NM_201268
Function Serine protease that catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF, GNPTAB and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg- Ser-Val-Leu (SERBP-2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes. {ECO:0000269|PubMed:21719679}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:9990022}; Single-pass type I membrane protein {ECO:0000269|PubMed:9990022}. Golgi apparatus membrane {ECO:0000269|PubMed:9990022}; Single-pass type I membrane protein {ECO:0000269|PubMed:9990022}. Note=May sort to other organelles, including lysosomal and/or endosomal compartments.
Tissue Specificity Widely expressed.
Top Pathways Protein processing in endoplasmic reticulum

SKI-1 (A-11) - sc-271916 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SKI-1 (A-11): sc-271916. Western blot analysis of SKI-1 expression in H4 (A), HeLa (B), T98G (C), K-562 (D) and U-87 MG (E) whole cell lysates.

SKI-1 (H-300) - sc-20757 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SKI-1 (H-300): sc-20757. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

SKI-1 (D-19) - sc-9786 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SKI-1 (D-19): sc-9786. Immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic staining of glandular cells.

SKI-1 (N-17) - sc-9785 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SKI-1 (N-17): sc-9785. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.

Anti-S1P antibody - ab140592 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-S1P antibody (ab140592) at 1 µg/mlLane 1 : Liver (Mouse) Tissue LysateLane 2 : Liver (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-S1P antibody - ab59869 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-S1P antibody (ab59869) at 1/1000 dilutionLane 1 : Tissue lysate prepared from rat liver (1:4) at 20 µlLane 2 : Tissue lysate prepared from mouse liver(10mg/ml) at 20 µlLane 3 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (serum free) at 10 µlLane 4 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with TPA) at 10 µlLane 5 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with IL1-beta) at 10 µlLane 6 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with TNF-alpha) at 10 µlLane 7 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with RA) at 10 µl

Anti-S1P antibody - ab59870 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-S1P antibody (ab59870) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-S1P antibody - ab59874 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-S1P antibody (ab59874) at 1/1000 dilutionLane 1 : Tissue lysate prepared from rat liver (1:4) at 20 µlLane 2 : Tissue lysate prepared from mouse liver (10 mg/ml) at 20 µlLane 3 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (serum free) at 10 µlLane 4 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with TPA) at 10 µlLane 5 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with IL1-beta) at 10 µlLane 6 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with TNF-alpha) at 10 µlLane 7 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with RA) at 10 µl

Anti-S1P antibody - Cytoplasmic domain - ab59883 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-S1P antibody - Cytoplasmic domain (ab59883) at 1/1000 dilutionLane 1 : Tissue lysate prepared from rat liver (1:4) at 20 µlLane 2 : Tissue lysate prepared from mouse liver (10mg/ml) at 20 µlLane 3 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (serum free) at 10 µlLane 4 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with TPA) at 10 µlLane 5 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with IL1-beta) at 10 µlLane 6 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (treated with TNF-alpha) at 10 µlLane 7 : Whole cell lysate prepared from human hepatoma SK-HEP-1 cells (1:4) (RA) at 10 µl

Anti-MBTPS1 / S1P Antibody (aa246-355) - LS-C308907 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-MBTPS1 / S1P Antibody - LS-C346377 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of U20S cell using MBTPS1 antibody. Blue: DAPI for nuclear staining.

MBTPS1 Antibody - NBP1-81547 from Novus Biologicals

Host Rabbit
Clonality Polyclonal
Western Blot: MBTPS1 Antibody [NBP1-81547] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp

MBTPS1 Antibody (2E6) - H00008720-M07 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E6

Anti-MBTPS1 antibody produced in rabbit - HPA006239 from Sigma-Aldrich

Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MBTPS1: <B>Cat. No. HPA006239</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human stomach shows moderate cytoplasmic positivity in glandular cells.

Monoclonal Anti-MBTPS1 antibody produced in mouse - SAB1401436 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E6
<B>Immunofluorescence</B><BR/>Monoclonal Anti-MBTPS1: <B>Cat. No. SAB1401436</B>: Antibody concentration: 10 μg/mL using HeLa cell.

MBTPS1-Antibody - AP50769 from Abgent, a WuXi AppTec company

Prices $295.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysate from U-87 MG cell line,using MBTPS1 Antibody(AP50769). AP50769 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.

MBTPS1 antibody [C1C3] - GTX117475 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal

MBTPS1 Antibody (OAAB18562) - OAAB18562 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

MBTPS1 Antibody (OAAF03817) - OAAF03817 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

MBTPS1 antibody - orb128343 from Biorbyt

Prices $85.00, $255.00, $425.00
Sizes 25 μg (Trial Size), 100 μg, 200 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat/COLO205 cells using MBTPS1 antibody
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