FBXW8
| Gene Symbol | FBXW8 |
|---|---|
| Entrez Gene | 26259 |
| Alt Symbol | FBW6, FBW8, FBX29, FBXO29, FBXW6 |
| Species | Human |
| Gene Type | protein-coding |
| Description | F-box and WD repeat domain containing 8 |
| Other Description | F-box and WD-40 domain protein 8|F-box and WD-40 domain-containing protein 8|F-box only protein 29|F-box/WD repeat-containing protein 8 |
| Swissprots | Q8N3Y1 Q9UK95 |
| Accessions | EAW98094 EAW98095 EAW98096 EAW98097 Q8N3Y1 AF176707 AAF03129 AK055849 AK289621 BAF82310 BC037296 AAH37296 BC045190 XM_005253866 XP_005253923 XM_005253867 XP_005253924 XM_005253868 XP_005253925 XM_011538172 XP_011536474 XR_944517 XR_944518 NM_012174 NP_036306 NM_153348 NP_699179 |
| Function | Substrate-recognition component of a Cul7-RING ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain (PubMed:21572988). Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed:18498745). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradatation, thereby affecting cell proliferation and differentiation (PubMed:24362026). Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions (PubMed:24793695). {ECO:0000269|PubMed:18498745, ECO: |
| Subcellular Location | Cytoplasm, perinuclear region {ECO:0000269|PubMed:21572988}. Golgi apparatus {ECO:0000269|PubMed:21572988}. Note=Colocalizes with CUL7 at the Golgi apparatus in neurons. {ECO:0000250}. |
| Top Pathways | Ubiquitin mediated proteolysis |
FBXO29 CRISPR/Cas9 KO Plasmid (h) - sc-407781 from Santa Cruz Biotechnology
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FBXO29 HDR Plasmid (h) - sc-407781-HDR from Santa Cruz Biotechnology
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FBXO29 Double Nickase Plasmid (h) - sc-407781-NIC from Santa Cruz Biotechnology
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FBXO29 Double Nickase Plasmid (h2) - sc-407781-NIC-2 from Santa Cruz Biotechnology
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FBXO29 CRISPR Activation Plasmid (h) - sc-407781-ACT from Santa Cruz Biotechnology
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FBXO29 CRISPR Activation Plasmid (h2) - sc-407781-ACT-2 from Santa Cruz Biotechnology
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FBXO29 Lentiviral Activation Particles (h) - sc-407781-LAC from Santa Cruz Biotechnology
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FBXO29 Lentiviral Activation Particles (h2) - sc-407781-LAC-2 from Santa Cruz Biotechnology
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FBXO29 siRNA (h) - sc-95763 from Santa Cruz Biotechnology
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FBXO29 shRNA Plasmid (h) - sc-95763-SH from Santa Cruz Biotechnology
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FBXO29 shRNA (h) Lentiviral Particles - sc-95763-V from Santa Cruz Biotechnology
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FBXW8 RNAi - H00026259-R01 from Novus Biologicals
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FBXW8 RNAi - H00026259-R02 from Novus Biologicals
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MISSION® esiRNA - EHU005861 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN8577 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND8577 from Sigma-Aldrich
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FBXW8 siRNA (Human) - CRH8784 from Cohesion Biosciences
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FBXW8 - MBS8205561 from MyBioSource
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shRNA set against Human FBXW8 (NM_012174.1) - SHH293577 from Creative biogene
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shRNA set against Human FBXW8(NM_012174.1) - SHH053849 from Creative biogene
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shRNA set against Human FBXW8(NM_153348.2) - SHH053885 from Creative biogene
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Human FBXW8 siRNA - orb269286 from Biorbyt
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FBXW8 shRNA Plasmid (Human) - CHH8784 from Cohesion Biosciences
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FBXW8 miRNA 3'UTR clone - MiUTR1H-03579 from Creative biogene
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FBXW8 miRNA 3'UTR clone - MiUTR1H-03580 from Creative biogene
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