MED30

Gene Symbol MED30
Entrez Gene 90390
Alt Symbol MED30S, THRAP6, TRAP25
Species Human
Gene Type protein-coding
Description mediator complex subunit 30
Other Description TRAP/Mediator complex component TRAP25|mediator of RNA polymerase II transcription subunit 30|putative mediator of RNA polymerase II transcription subunit 30|thyroid hormone receptor associated protein 6|thyroid hormone receptor-associated protein 6|thyroid hormone receptor-associated protein complex 25 kDa component
Swissprots C6GKU9 Q96HR3
Accessions EAW91970 EAW91971 Q96HR3 AA812233 AW160991 AY083305 AAL89787 BC008226 AAH08226 DQ891518 ABM82444 DQ894708 ABM85634 FM179284 CAQ76893 XM_011517360 XP_011515662 NM_001282986 NP_001269915 NM_080651 NP_542382
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:11909976, ECO:0000269|PubMed:16595664}.
Subcellular Location Nucleus {ECO:0000305}.
Tissue Specificity Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. {ECO:0000269|PubMed:11909976}.

Med30 (A-11) - sc-374480 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Med30 (A-11): sc-374480. Western blot analysis of Med30 expression in non-transfected: sc-117752 (A) and human Med30 transfected: sc-174753 (B) 293T whole cell lysates.

Med30 (A-19) - sc-98002 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Med30 (A-19): sc-98002. Western blot analysis of Med30 expression in K-562 nuclear extract.

Med30 (C-6) - sc-393289 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Med30 (C-6): sc-393289. Western blot analysis of Med30 expression in non-transfected 293T: sc-117752 (A), human Med30 transfected 293T: sc-174753 (B), HCT-116 (C) and TE671 (D) whole cell lysates and K-562 nuclear extract (E).

Med30 (FL-178) - sc-367551 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Med30 (FL-178): sc-367551. Western blot analysis of Med30 expression in CCRF-CEM (A), PC-3 (B), Caki-1 (C) and Hep G2 (D) whole cell lysates and mouse pancreas tissue extract (E).

Anti-MED30 / TRAP25 antibody - ab192523 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MED30 / TRAP25 antibody (ab192523)Lane 1 : HEK293T whole cell lysateLane 2 : Raw264.7 whole cell lysateLane 3 : H9C2 whole cell lysate

Anti-MED30 / TRAP25 antibody - ab67644 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-MED30 / TRAP25 antibody (ab67644) at 1/500 dilutionLane 1 : MED30 / TRAP 25 transfected 293T cell lysateLane 2 : Untransfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L) HRP conjugate

Anti-MED30 / TRAP25 antibody - C-terminal - ab186967 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MED30 / TRAP25 antibody - C-terminal (ab186967) at 1 µg/ml + ACHN whole cell lysate at 10 µg

Anti-MED30 Antibody (aa107-133, APC) - LS-C262934 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED30 Antibody (aa107-133, AP) - LS-C262935 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED30 Antibody (aa107-133, Biotin) - LS-C262936 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED30 Antibody (aa107-133, Azide-free, HRP) - LS-C262938 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED30 Antibody (aa107-133, FITC) - LS-C262939 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED30 Antibody (aa106-133) - LS-C322942 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED30 Antibody (aa107-133, PE) - LS-C262940 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED30 Antibody - LS-C308939 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MED30 Antibody (aa104-153) - LS-C206206 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MED30 antibody Western Blot of ACHN. Antibody dilution: 1 ug/ml.

Anti-MED30 Antibody (aa107-133) - LS-C158125 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MED30 Antibody western blot of WiDr cell line lysates (35 ug/lane). The MED30 antibody detected the MED30 protein (arrow).

Anti-MED30 Antibody - LS-C340993 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

MED30 Antibody - H00090390-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: MED30 Antibody [H00090390-B01] - analysis of THRAP6 expression in transfected 293T cell line by THRAP6 MaxPab polyclonal antibody (B01). Lane 1: THRAP6 transfected lysate (20.30 KDa). Lane 2: Non-transfected lysate.

MED30 Antibody - H00090390-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: MED30 Antibody [H00090390-B01P] - Analysis of MED30 expression in transfected 293T cell line by MED30 polyclonal antibody. Lane 1: THRAP6 transfected lysate(19.58 KDa). Lane 2: Non-transfected lysate.

Anti-MED30 (61-75) antibody produced in rabbit - SAB1102679 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human MED30 was separated on SDS-PAGE, blotted with Anti-MED30 (61-75) (<B>Cat. No. SAB1102679</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:1,000 + MED30 immunizing peptide (human, 61-75)

Anti-MED30 antibody produced in mouse - SAB1408169 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MED30: <B>Cat. No. SAB1408169</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~19.6 kDa using MED30 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

MED30 Antibody - C-terminal region (ARP73175_P050) - ARP73175_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

MED30-Antibody-Center - AP19149c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
MED30 Antibody (Center) (Cat. #AP19149c) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MED30 antibody detected the MED30 protein (arrow).

MED30 antibody [N1C3] - GTX117030 from GeneTex

Host Rabbit
Clonality Polyclonal
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