NELFA

Gene Symbol NELFA
Entrez Gene 7469
Alt Symbol NELF-A, P/OKcl.15, WHSC2
Species Human
Gene Type protein-coding
Description negative elongation factor complex member A
Other Description negative elongation factor A|wolf-Hirschhorn syndrome candidate 2 protein
Swissprots A2A2T1 Q9H3P2 O95392
Accessions EAW82545 EAW82546 EAW82547 Q9H3P2 AB044549 BAB18651 AF101434 AAC72982 AF131751 AAD20034 AI218582 AI560345 AK001304 BAG50890 AK094040 BAG52802 AK095737 BAG53117 AK126056 BAG54283 AK222853 BAD96573 BC002764 AAH02764 DB082393 EU831413 ACE87100 EU831506 ACE86425 XM_006713916 XP_006713979 XM_011513561 XP_011511863 NM_005663 NP_005654
Function Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. {ECO:0000269|PubMed:10199401, ECO:0000269|PubMed:12563561, ECO:0000269|PubMed:12612062}.
Subcellular Location Nucleus {ECO:0000269|PubMed:11150502}.
Tissue Specificity Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney. {ECO:0000269|PubMed:10409432, ECO:0000269|PubMed:11150502, ECO:0000269|PubMed:12612062}.

NELF-A (A-10) - sc-271960 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NELF-A (A-10): sc-271960. Western blot analysis of NELF-A expression in non-transfected: sc-117752 (A) and human NELF-A transfected: sc-177614 (B) 293T whole cell lysates and PMA treated Jurkat nuclear extract (C).

NELF-A (A-20) - sc-23599 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NELF-A (A-20): sc-23599. Western blot analysis of NELF-A expression in DU 145 nuclear extract.

NELF-A (F-2) - sc-271731 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NELF-A (F-2): sc-271731. Western blot analysis of NELF-A expression in non-transfected: sc-117752 (A) and mouse NELF-A transfected: sc-122003 (B) 293T whole cell lysates.

NELF-A (G-11) - sc-365004 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NELF-A (G-11): sc-365004. Western blot analysis of NELF-A expression in DU 145 (A) and Jurkat (B) whole cell lysates.

NELF-A (H-240) - sc-32911 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NELF-A (H-240): sc-32911. Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymus tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Anti-WHSC2 antibody - ab167675 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-WHSC2 antibody (ab167675) at 1 µg/mlLane 1 : WHSC2 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-WHSC2 antibody - ab85852 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling WHSC2 with ab85852 at 1/1000 (1µg/ml). Detection: DAB.

Anti-WHSC2 antibody - ab88870 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-WHSC2 antibody (ab88870) at 1 µg/ml + Jurkat cell lysate at 50 µg

Anti-WHSC2 antibody - ab123674 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-WHSC2 antibody (ab123674) at 1/100 dilution + NCI-H460 cell lysate at 35 µg

Anti-WHSC2 antibody - ab128317 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-WHSC2 antibody (ab128317) at 1 µg/ml + Mouse brain lysate at 10 µg

Anti-NELFA / WHSC2 Antibody (aa104-291) - LS-C32443 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µl
Host Chicken
Clonality Polyclonal

Anti-NELFA / WHSC2 Antibody (aa261-290, Biotin) - LS-C242744 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NELFA / WHSC2 Antibody (aa261-290, APC) - LS-C242742 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NELFA / WHSC2 Antibody (aa261-290, AP) - LS-C242743 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NELFA / WHSC2 Antibody (aa261-290, FITC) - LS-C242745 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NELFA / WHSC2 Antibody (aa261-290, PE) - LS-C242749 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NELFA / WHSC2 Antibody (aa261-290, Azide-free, HRP) - LS-C247254 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NELFA / WHSC2 Antibody (aa261-290) - LS-C330298 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NELFA / WHSC2 Antibody (aa343-392) - LS-C151414 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NELFA / WHSC2 antibody Western Blot of Mouse Brain.

Anti-NELFA / WHSC2 Antibody (aa247-296) - LS-C201757 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human PANC1. WHSC2 antibody dilution 1.0 ug/ml.

Anti-NELFA / WHSC2 Antibody (aa251-279) - LS-C160621 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
WHSC2 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The WHSC2 antibody detected the WHSC2 protein (arrow).

Anti-NELFA / WHSC2 Antibody (aa499-549) - LS-C286619 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human NELFA by Immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-NELFA used at a dilution of 1:250.

Anti-NELFA / WHSC2 Antibody (aa499-549) - LS-C288684 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human NELFA by Immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-NELFA used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-NELFA / WHSC2 Antibody (aa280-511, clone 6B11H8) IHC-plus™ - LS-B10744 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 6B11H8
Anti-NELFA / WHSC2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10744 concentration 10 ug/ml.

Anti-NELFA / WHSC2 Antibody (aa280-511, clone 6B11H8) - LS-C191814 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6B11H8
IHC of paraffin-embedded colon cancer tissues using WHSC2 mouse monoclonal antibody with DAB staining.
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