SLC5A8

Gene Symbol SLC5A8
Entrez Gene 160728
Alt Symbol AIT, SMCT, SMCT1
Species Human
Gene Type protein-coding
Description solute carrier family 5 (sodium/monocarboxylate cotransporter), member 8
Other Description apical iodide transporter|electrogenic sodium monocarboxylate cotransporter|sodium iodide-related cotransporter|sodium-coupled monocarboxylate transporter 1|solute carrier family 5 (iodide transporter), member 8|solute carrier family 5 member 8
Swissprots Q7Z2H9 Q2TB99 Q8N695
Accessions AAP46194 EAW97648 EAW97649 EAW97650 EAW97651 Q8N695 AF536216 AAP46193 AK075263 AK313788 BAG36525 AY081220 AAL88746 BC110492 AAI10493 XR_944503 XR_952100 NM_145913 NP_666018
Function Acts as an electrogenic sodium (Na(+)) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D- hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na(+):substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D- lactate and monocarboxylate drugs from the intestinal tract. Acts as a tumor suppressor, suppressing colony formation in colon cancer, prostate cancer and glioma cell lines. May play a critical role in the entry of L-lactate and ketone bodies into neurons by a process driven by an electrochemical Na(+) gradient and
Subcellular Location Apical cell membrane {ECO:0000269|PubMed:12107270, ECO:0000269|PubMed:15001644, ECO:0000269|PubMed:17245649}; Multi-pass membrane protein {ECO:0000269|PubMed:12107270, ECO:0000269|PubMed:15001644, ECO:0000269|PubMed:17245649}. Note=Expressed at the apical membrane of normal tall thyrocytes and of colonic epithelial cells. {ECO:0000269|PubMed:12107270, ECO:0000269|PubMed:15001644, ECO:0000269|PubMed:17245649}.
Tissue Specificity Expressed in normal thyroid, localized at the apical pole of thyroid cells facing the colloid lumen, but expression profoundly decreased in thyroid carcinomas. Expressed in normal colon but absent in colon aberrant crypt foci and colon cancers. Present in normal kidney cortex, brain, prostate, gastric mucosa and breast tissue but was significantly down-regulated in primary gliomas, gastric cancer, prostate tumors and breast tumors. {ECO:0000269|PubMed:12107270, ECO:0000269|PubMed:12829793, ECO:0000269|PubMed:14966140, ECO:0000269|PubMed:15001644, ECO:0000269|PubMed:15090606, ECO:0000269|PubMed:15361710, ECO:0000269|PubMed:15867356, ECO:0000269|PubMed:17178845, ECO:0000269|PubMed:17245649, ECO:0000269|PubMed:18037591}.

SLC5A8 (H-18) - sc-34194 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SLC5A8 (N-14) - sc-34188 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-SLC5A8 antibody - ab99064 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-SLC5A8 antibody (ab99064) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg

Anti-SLC5A8 antibody - Carboxyterminal end - ab104992 from Abcam

Host Rabbit
Clonality Polyclonal
ICC/IF image of ab104992 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104992 at 1/1000 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-SLC5A8 / AIT Antibody (aa548-574, AP) - LS-C217579 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC5A8 / AIT Antibody (aa548-574, FITC) - LS-C217581 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC5A8 / AIT Antibody (aa548-574, Biotin) - LS-C217582 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC5A8 / AIT Antibody (aa548-574, APC) - LS-C217580 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC5A8 / AIT Antibody (aa548-574, PE) - LS-C217584 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC5A8 / AIT Antibody (aa548-574) - LS-C330153 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC5A8 / AIT Antibody (aa548-574, Azide-free, HRP) - LS-C217583 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC5A8 / AIT Antibody (aa431-480) - LS-C110862 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SLC5A8 antibody LS-C110862 Western blot of OVCAR-3 cell lysate.

Anti-SLC5A8 / AIT Antibody (aa548-574) - LS-C157934 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Mouse Slc5a8 Antibody western blot of mouse bladder tissue lysates (35 ug/lane). The Mouse Slc5a8 antibody detected the Mouse Slc5a8 protein (arrow).

Anti-SLC5A8 / AIT Antibody (C-Terminus) - LS-C345985 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of SLC5A8 Antibody with human Fetal Kidney lysate.

SLC5A8/SMCT1 Antibody (903502) - MAB8398 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 903502
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human SLC5A8/SMCT1 Monoclonal Antibody (Catalog # MAB8398, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

SLC5A8/SMCT1 Antibody - NBP1-62527 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SLC5A8 Antibody [NBP1-62527] - OVCAR-3 cell lysate, concentration 0.2-1 ug/ml.

SLC5A8 Polyclonal Antibody - bs-6106R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-SLC5A8 Polyclonal Antibody, Unconjugated (bs-6106R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

SLC5A8 antibody - middle region (ARP44110_P050) - ARP44110_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SLC5A8 Antibody - C-terminal region (ARP44111_P050) - ARP44111_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Human SLC5A8/SMCT1 Antibody - MAB8398 from R&D Systems

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clone 903502
Detection of SLC5A8/SMCT1 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human SLC5A8/SMCT1 Monoclonal Antibody (Catalog # MAB8398, filled histogram) or isotype control antibody (Catalog # MAB002 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B ).

Human SLC5A8 MAb (Clone 903502), Mouse IgG1 - MAB8398-SP from R&D Systems

Host Mouse
Clonality Monoclonal
Clone 903502
Detection of SLC5A8/SMCT1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human SLC5A8/SMCT1 Monoclonal Antibody (Catalog # MAB8398, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

SLC5A8 antibody - orb101289 from Biorbyt

Prices $255.00, $340.00
Sizes 100 μg, 200 μg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed and paraffin embedded human colon carcinoma tissue (dilution at:1:200) using SLC5A8 antibody

SLC5A8 Antibody (OABF01657) - OABF01657 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SLC5A8 antibody - 70R-20364 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

SLC5A8 antibody - 70R-7372 from Fitzgerald

Host Rabbit
Clonality Polyclonal
Search more