SLC17A7

Gene Symbol SLC17A7
Entrez Gene 57030
Alt Symbol BNPI, VGLUT1
Species Human
Gene Type protein-coding
Description solute carrier family 17 (vesicular glutamate transporter), member 7
Other Description brain-specific Na(+)-dependent inorganic phosphate cotransporter|brain-specific Na-dependent inorganic phosphate cotransporter|solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7|solute carrier family 17 member 7|vesicular glutamate transporter 1
Swissprots Q6PCD0 Q9P2U7 B4DG46 B4DFR9
Accessions EAW52485 Q9P2U7 AB032436 BAA92875 AK057879 AK091061 AK095659 AK289622 BAF82311 AK294226 BAG57530 AK294405 BAG57657 BC059379 AAH59379 BG701511 BQ636968 BU732143 NM_020309 NP_064705
Function Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. {ECO:0000269|PubMed:10820226}.
Subcellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250}. Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Cell junction, synapse, synaptosome {ECO:0000250}.
Tissue Specificity Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe. {ECO:0000269|PubMed:10820226}.
Top Pathways Synaptic vesicle cycle, Glutamatergic synapse, Nicotine addiction, Retrograde endocannabinoid signaling

VGLUT1 Antibody - 12331 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from rat synaptosome, mouse brain, and rat brain tissue using VGLUT1 Antibody.

BNPI (C-15) - sc-13320 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BNPI (C-15): sc-13320. Western blot analysis of BNPI expression in rat cerebellum extract.

BNPI (A-8) - sc-377425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BNPI (A-8): sc-377425. Western blot analysis of BNPI expression in rat cerebellum (A) and human brain (B) tissue extracts.

BNPI (H-55) - sc-28940 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BNPI (H-55): sc-28940. Western blot analysis of BNPI expression in mouse brain tissue extract.

BNPI (F-15) - sc-23168 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-VGluT1 antibody - ab104898 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC-P image of VGlut1 staining on goat cerebellum section using ab104898 (1:4000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab104898 was diluted 1:4000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (undiluted).See Abreview

Anti-VGluT1 antibody - ab72311 from Abcam

Prices $398.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal
ab72311 at 30µg/ml staining VGLUT1 in rat brain tissue by Immunohistochemistry (free floating section).

Anti-VGluT1 antibody - ab104899 from Abcam

Prices $378.00
Sizes 100 µl
Host Goat
Clonality Polyclonal
ab104899 staining VGluT1 in rat cerebellum by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 10% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 14 hours at 25°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.See Abreview

Anti-VGluT1 antibody - ab79774 from Abcam

Host Sheep
Clonality Polyclonal
Anti-VGluT1 antibody (ab79774) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µgSecondaryRabbit polyclonal Secondary Antibody to Sheep IgG - H&L (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-VGluT1 antibody - ab130445 from Abcam

Host Chicken
Clonality Polyclonal

Anti-VGluT1 antibody - ab85922 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VGluT1 antibody (ab85922) at 1 µg/mlLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-VGluT1 antibody - ab110139 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab110139, at 3.75 µg/ml, staining VGluT1 in Human brain (cortex) tissue by immunohistochemistry. After incubation with ab110139, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Anti-VGluT1 antibody [EPR10953] - C-terminal - ab180188 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10953
All lanes : Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188) at 1/1000 dilutionLane 1 : Fetal brain lysateLane 2 : Neuro-2a lysateLane 3 : C6 lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-VGluT1 antibody [McKA1] - ab150347 from Abcam

Host Mouse
Clonality Monoclonal
Clone McKA1

Anti-VGluT1 antibody [N28-9] - ab134283 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone N28-9
Overlay histogram showing SHSY-5Y cells stained with ab134283 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134283, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-VGluT1 antibody [N28-9] (Alexa Fluor® 647) - ab193596 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone N28-9
IF image of VGluT1 staining in a section of frozen rat brain (cerebellum). The section was air dried on the bench for 30 minutes and blocked for 2 hours in 1XPBS/0.1% Tween 20/1% BSA/0.3M Glycine buffer. The sample was then incubated overnight at +4°C with ab193596 at 1/50 dilution (shown in red). DAPI- containing mounting media was added to the sample (shown in blue). The inset negative control image is taken from an identical assay without primary antibody.

Anti-SLC17A7 / VGLUT1 Antibody - LS-C179267 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC17A7 / VGLUT1 Antibody (N-Terminus) - LS-C15451 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC17A7 / VGLUT1 Antibody (N-Terminus) - LS-C15449 from LifeSpan Bioscience

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC17A7 / VGLUT1 Antibody (aa500-560) - LS-C95237 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC17A7 / VGLUT1 Antibody (aa500-560) - LS-C95267 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Sheep
Clonality Polyclonal

Anti-SLC17A7 / VGLUT1 Antibody (clone S28-9) - LS-C150469 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S28-9

Anti-SLC17A7 / VGLUT1 Antibody (aa493-560, clone S28-9, Atto 390) - LS-C231623 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S28-9

Anti-SLC17A7 / VGLUT1 Antibody (aa493-560, clone S28-9, Atto 488) - LS-C231624 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S28-9

Anti-SLC17A7 / VGLUT1 Antibody (aa493-560, clone S28-9, Atto 655) - LS-C231628 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S28-9
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