ATG3

Gene Symbol ATG3
Entrez Gene 64422
Alt Symbol APG3, APG3-LIKE, APG3L, PC3-96
Species Human
Gene Type protein-coding
Description autophagy related 3
Other Description 2610016C12Rik|ATG3 autophagy related 3 homolog|autophagy-related protein 3|hApg3|ubiquitin-like-conjugating enzyme ATG3
Swissprots Q6PKC5 Q9H6L9 Q9NT62
Accessions EAW79668 EAW79669 EAW79670 EAW79671 Q9NT62 AA448974 AB079384 BAB90843 AF202092 AAG35611 AK025778 BAB15237 AK123511 AK307432 AK308919 AL137515 CAB70781 AW408464 BC002830 AAH02830 BC024221 AAH24221 BC043267 DC311625 DQ893424 ABM84350 DQ896774 ABM87773 XM_011513074 XP_011511376 NM_001278712 NP_001265641 NM_022488 NP_071933
Function E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriola
Subcellular Location Cytoplasm {ECO:0000269|PubMed:11825910}.
Tissue Specificity Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. {ECO:0000269|PubMed:11825910}.
Top Pathways Regulation of autophagy

Atg3 Antibody - 3415 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Atg3 Antibody.

Atg3 (D-9) - sc-393623 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Atg3 (D-9): sc-393623. Western blot analysis of Atg3 expression in Jurkat (A), K-562 (B), HeLa (C) and Hep G2 (D) whole cell lysates.

Atg3 (A-3) - sc-393660 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Atg3 (A-3): sc-393660. Western blot analysis of Atg3 expression in Jurkat (A), K-562 (B), HeLa (C) and HL-60 (D) whole cell lysates.

Atg3 (Y-20) - sc-70139 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Atg3 (Y-20): sc-70139. Western blot analysis of Atg3 expression in Jurkat whole cell lysate.

Atg3 (E-20) - sc-70136 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Atg3 (AA6) - sc-100508 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Atg3 (AA6): sc-100508. Western blot analysis of Atg3 expression in Jurkat whole cell lysate.

Anti-Apg3 antibody - ab62111 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Apg3 antibody - ab72003 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Apg3 antibody - ab56409 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Apg3 antibody [1377CT239.6.1.12] - ab202304 from Abcam

Prices $359.00
Sizes 40 µl
Host Mouse
Clonality Monoclonal
Clone 1377CT239.6.1.12
All lanes : Anti-Apg3 antibody [1377CT239.6.1.12] (ab202304) at 1/1000 dilutionLane 1 : THP-1 lysateLane 2 : Mouse liver lysateLane 3 : Mouse testis lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG H&L(HRP) at 1/10000 dilution

Anti-Apg3 antibody [EPR4801] - ab108251 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4801
ab108251 staining Apg3 in Mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 12 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

Anti-Apg3 antibody [EPR4801] (Alexa Fluor® 488) - ab201518 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4801
ab201518 staining Apg3 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201518 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Apg3 antibody [EPR4801] (DyLight® 488) - ab139883 from Abcam

Prices $337.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4801
Immunofluorescent analysis of HeLa cells labelling Apg3 with ab139883 at 1/100 dilution (green), Dapi staining shown in blue.

Anti-Apg3 antibody [EPR4802] - ab108282 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4802
Flow cytometric analysis of permeabilized Jurkat cells using ab108282 at 1/10 (red) or a rabbit IgG (negative) (green).

Anti-ATG3 Antibody (N-Terminus) - LS-C69120 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ATG3 Antibody - LS-C93847 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Sheep
Clonality Polyclonal

Anti-ATG3 Antibody - LS-C125091 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-ATG3 Antibody (aa192-210) - LS-C148921 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ATG3 Antibody - LS-C153219 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ATG3 Antibody (clone 1G3) - LS-C139422 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1G3
ATG3 monoclonal antibody (M04), clone 1G3. Western blot of ATG3 expression in HepG2.

Anti-ATG3 Antibody (aa215-264) - LS-C117262 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ATG3 antibody LS-C117262 Western blot of 293T cell lysate.

Anti-ATG3 Antibody (aa91-140) - LS-C135482 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ATG3 antibody LS-C135482 Western blot of HeLa lysate.

Anti-ATG3 Antibody - LS-C163589 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Dot blot of anti-APG3-cleaved antibody on nitrocellulose membrane. 50ng of Cleaved-peptide or Non Cleaved-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Anti-ATG3 Antibody (AP) - LS-C221441 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATG3 Antibody (aa17-32) - LS-C188037 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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