MPHOSPH8

Gene Symbol MPHOSPH8
Entrez Gene 54737
Alt Symbol HSMPP8, TWA3, mpp8
Species Human
Gene Type protein-coding
Description M-phase phosphoprotein 8
Other Description M-phase phosphoprotein, mpp|M-phase phosphoprotein, mpp8|two hybrid-associated protein 3 with RanBPM
Swissprots B7Z6F9 Q5JPE5 Q86TK3 Q5JTQ0 Q99549 Q96MK4 Q9BTP1
Accessions EAX08228 EAX08229 EAX08230 Q99549 AJ293409 AK056785 BAB71284 AK092556 BAG52572 AK300258 BAH13245 AL832864 CAI46172 BC003542 AAH03542 BC017322 BC046214 AAH46214 DA672302 R60056 X98259 CAA66912 XM_005266436 XP_005266493 XM_011535124 XP_011533426 XR_941609 NM_017520 NP_059990
Function Involved in transcriptional regulation. Specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene. Mediates down-regulation of CDH1 expression. {ECO:0000269|PubMed:20871592}.
Subcellular Location Nucleus {ECO:0000269|PubMed:20871592, ECO:0000269|PubMed:8885239}. Note=Detected on heterochromatin. Detected on nucleosomes.

MPHOSPH8 (C-8) - sc-398598 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MPHOSPH8 (C-8): sc-398598. Western blot analysis of MPHOSPH8 expression in MIA PaCa-2 (A), HeLa (B), RT-4 (C) and U-251-MG (D) whole cell lysates and HeLa nuclear extract (E).

MPHOSPH8 (C-20) - sc-84207 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

MPHOSPH8 (H-196) - sc-368979 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-MPP8 antibody - ab122575 from Abcam

Prices $443.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab122575, at 1/500 dilution, staining MPP8 in paraffin-embedded Human testis tissue by Immunohistochemistry.

Anti-MPP8 antibody - ab122611 from Abcam

Prices $443.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab122611, at 1/1000 dilution, staining MPP8 in Paraffin-embedded Human Colon tissue by Immunohistochemistry.

Anti-MPP8 antibody - ab169612 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-MPP8 antibody (ab169612) at 1 µg/mlLane 1 : MPP8 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-MPP8 antibody - ab124499 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MPP8 antibody (ab124499) at 1 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate (ab14860)Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MPHOSPH8 Antibody (aa200-250) - LS-C286146 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MPHOSPH8 Antibody (aa1-50) - LS-C289755 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

mpp8 Antibody - NBP1-92136 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

mpp8 Antibody - NBP1-92135 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: mpp8 Antibody [NBP1-92135] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

mpp8 Antibody - NBP1-71809 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: mpp8 Antibody [NBP1-71809] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-MPP8 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MPP8 was also immunoprecipitated by rabbit anti-MPP8 antibody NBP1-71808, which recognizes an upstream epitope.

mpp8 Antibody - NBP1-71808 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

mpp8 Antibody - H00054737-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: mpp8 Antibody [H00054737-B01] - analysis of MPHOSPH8 expression in transfected 293T cell line by MPHOSPH8 MaxPab polyclonal antibody. Lane 1: MPHOSPH8 transfected lysate (28.40 KDa). Lane 2: Non-transfected lysate.

mpp8 Antibody - H00054737-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MPHOSPH8 antibody produced in mouse - SAB1409462 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MPHOSPH8: <B>Cat. No. SAB1409462</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~28.4 kDa using MPHOSPH8 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-MPHOSPH8 antibody produced in rabbit - HPA039701 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MPHOSPH8: <B>Cat. No. HPA039701</B>: Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts and Leydig cells.

Anti-MPHOSPH8 antibody produced in rabbit - HPA040035 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MPHOSPH8: <B>Cat. No. HPA040035</B>: Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

MPHOSPH8 antibody - 70R-18575 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

MPHOSPH8 Polyclonal Antibody - A4792 from ABclonal

Prices $200.00, $480.00
Sizes 50 µg, 200 µg
Host Rabbit
Clonality Polyclonal

M-Phase Phosphoprotein 8 Antibody - abx113884 from Abbexa

Host Rabbit
Clonality Polyclonal

MPHOSPH8 Antibody - abx003627 from Abbexa

Host Rabbit
Clonality Polyclonal

MPHOSPH8 antibody - 16796-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
HeLa cells were subjected to SDS PAGE followed by western blot with 16796-1-AP(MPHOSPH8 antibody) at dilution of 1:1000

MPHOSPH8 antibody - AP32517PU-N from Acris Antibodies

Prices $360.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
RanBPM/Purified representative staining pattern/morphology in CD30+ human lymphoma. Tissue pretreated with citrate pH 6, antigen retrieval. Antibody diluted to 1/500, IHC-Select Detection with HRP-DAB. Immunoreactivity is seen predominantly in CD30+ R.S. cells.

MPHOSPH8 antibody - H00054737-B01 from Acris Antibodies

Prices $360.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot analysis of MPHOSPH8 expression in transfected 293T cell line (H00054737-T02) by MPHOSPH8 MaxPab polyclonal antibody. Lane 1: MPHOSPH8 transfected lysate(28.40 KDa). Lane 2: Non-transfected lysate.
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