TDG

Gene Symbol TDG
Entrez Gene 6996
Alt Symbol hTDG
Species Human
Gene Type protein-coding
Description thymine-DNA glycosylase
Other Description G/T mismatch-specific thymine DNA glycosylase
Swissprots Q8IUZ6 Q13569 Q8IZM3
Accessions AAN16399 EAW97727 EAW97728 EAW97729 Q13569 AI004247 AI452763 AK295387 BAG58341 AK299832 BAG61699 AK303631 BAG64639 AK309792 AK311673 AK313234 BAG36045 AL710869 BC001307 BC010945 BC019925 AAH19925 BC037557 AAH37557 BC071714 BC104477 AAI04478 CB047650 CN274338 DB162106 DN601672 DQ786320 XM_005269125 XP_005269182 XM_011538714 XP_011537016 XM_011538715 XP_011537017 XR_429113 NM_003211 NP_003202 NM_001008411
Function DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5- hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5- methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition t
Subcellular Location Nucleus {ECO:0000269|PubMed:8662714}.
Top Pathways Base excision repair

TDG (D-11) - sc-376652 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TDG (D-11): sc-376652. Western blot analysis of TDG expression in HeLa nuclear extract (A) and Raji whole cell lysate (B).

TDG (H-114) - sc-292440 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TDG (H-114): sc-292440. Western blot analysis of TDG expression in non-transfected: sc-117752 (A) and human TDG transfected: sc-114834 (B) 293T whole cell lysates.

TDG (F-13) - sc-22845 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TDG (A-14) - sc-22847 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TDG (A-14): sc-22847. Western blot analysis of TDG expression in non-transfected: sc-117752 (A) and mouse TDG transfected: sc-123966 (B) 293T whole cell lysates.

Anti-Thymine DNA glycosylase antibody - ab167641 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Thymine DNA glycosylase antibody (ab167641) at 1 µg/mlLane 1 : Thymine DNA glycosylase transfected 293T cell line Lane 2 : Non-transfected 293T cell line

Anti-Thymine DNA glycosylase antibody - ab183644 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Lane 1 : Lane 2 : Lane 1 : 293T whole cell lysateLane 2 : 293T whole cell lysate, transtected with Thymine DNA glycosylaseLysates/proteins at 10 µg per lane.

Anti-Thymine DNA glycosylase antibody [AT2F7] - ab175968 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AT2F7
Anti-Thymine DNA glycosylase antibody [AT2F7] (ab175968) at 1/250 dilution + HeLa cell lysate at 30 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique

Anti-Thymine DNA glycosylase antibody [EPR8774] - ab154192 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8774
All lanes : Anti-Thymine DNA glycosylase antibody [EPR8774] (ab154192) at 1/5000 dilutionLane 1 : Mouse brain tissue at 20 µgLane 2 : mouse heart tissue at 20 µgLane 3 : mouse kidney tissueLane 4 : mouse spleen tissue at 20 µgLane 5 : rat brain tissue at 20 µgLane 6 : rat heart tissue at 20 µgLane 7 : rat kidney tissue at 20 µgLane 8 : rat spleen tissue at 20 µg

Anti-Thymine DNA glycosylase antibody - ab106301 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Thymine DNA glycosylase was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Thymine DNA glycosylase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab106301.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 50kDa; Thymine DNA glycosylase

Anti-Thymine DNA glycosylase antibody - ab65219 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Thymine DNA glycosylase antibody (ab65219) at 1/500 dilutionLane 1 : Jurkat cell extractLane 2 : Jurkat cell extract with immunising peptide at 10 µgLysates/proteins at 5 µg per lane.

Anti-Thymine DNA glycosylase antibody - ab93001 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Thymine DNA glycosylase antibody - N-terminal - ab135584 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Thymine DNA glycosylase antibody - N-terminal (ab135584) at 1/50 dilution + mouse lung tissue lysates at 35 µg

Anti-TDG / Thymine DNA Glycosylase Antibody (aa31-80) - LS-C119026 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from Jurkat cells, using TDG Antibody. The lane on the right is treated with the synthesized peptide.

Anti-TDG / Thymine DNA Glycosylase Antibody (Lys90) - LS-C176808 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of TDG (K90) pAb in extracts from Jurkat cells.

Anti-TDG / Thymine DNA Glycosylase Antibody - LS-C305941 from LifeSpan Bioscience

Prices $560.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of TDG / Thymine DNA Glycosylase antibody LS-X223194.

Anti-TDG / Thymine DNA Glycosylase Antibody (clone AT2F7) - LS-C200834 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AT2F7
Western Blot: The cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-TDG / Thymine DNA Glycosylase Antibody - LS-C335140 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of brain cell lysate using TDG antibody.

Anti-TDG / Thymine DNA Glycosylase Antibody (aa81-110) - LS-C99357 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of TDG antibody in mouse lung tissue lysates (35 ug/lane). TDG (arrow) was detected using the purified antibody.

Anti-TDG / Thymine DNA Glycosylase Antibody - LS-C334266 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using TDG antibody.

Anti-TDG / Thymine DNA Glycosylase Antibody (aa281-310) - LS-C99358 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of TDG antibody in K562 cell line lysates (35 ug/lane). TDG (arrow) was detected using the purified antibody.

Anti-TDG / Thymine DNA Glycosylase Antibody (aa360-410) - LS-C291004 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human TDG by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TDG antibody used for IP at 6 ug per reaction. TDG was also immunoprecipitated by rabbit anti-TDG antibody BL16331. For blotting immunoprecipitated TDG, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

TDG Antibody - NBP2-13423 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: TDG Antibody [NBP2-13423] - Analysis in human cell line HEK 293.

TDG Antibody - H00006996-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: TDG Antibody [H00006996-B01P] - Analysis of TDG expression in transfected 293T cell line by TDG polyclonal antibody. Lane 1: TDG transfected lysate(45.1 KDa). Lane 2: Non-transfected lysate.

TDG Antibody - NBP2-32248 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

TDG Antibody - NBP2-20627 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: TDG Antibody [NBP2-20627] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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