TGS1
| Gene Symbol | TGS1 |
|---|---|
| Entrez Gene | 96764 |
| Alt Symbol | NCOA6IP, PIMT, PIPMT |
| Species | Human |
| Gene Type | protein-coding |
| Description | trimethylguanosine synthase 1 |
| Other Description | CLL-associated antigen KW-2|PRIP-interacting protein PIPMT|PRIP-interacting protein with methyltransferase domain|PRIP-interacting protein with methyltransferase motif|SEREX-defined|cap-specific guanine-N2 methyltransferase|hepatocellular carcinoma-associated antigen 137|nuclear receptor coactivator 6 interacting protein|nuclear receptor coactivator 6-interacting protein|trimethylguanosine synthase|trimethylguanosine synthase homolog |
| Swissprots | Q8TDG9 A6NJQ5 Q96QU3 Q5GH23 Q96RS0 Q9H5V3 |
| Accessions | EAW86761 EAW86762 EAW86763 Q96RS0 AF286340 AAK83025 AF432215 AAL99922 AK026648 BAB15516 AK055178 BAG51481 AK314693 BAG37244 AL832426 AY028423 AAK27730 AY534911 AAT02709 BC011999 AAH11999 BC160105 AAI60105 BM853546 BX648548 DB082992 XM_005251328 XP_005251385 XM_006716485 XP_006716548 XM_006716486 XP_006716549 NM_024831 NP_079107 |
| Function | Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation. {ECO:0000269|PubMed:11517327, ECO:0000269|PubMed:11912212, ECO:0000269|PubMed:16687569, ECO:0000269|PubMed:18775984}. |
| Subcellular Location | Cytoplasm. Nucleus, Cajal body. Note=A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network. |
| Tissue Specificity | Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta. {ECO:0000269|PubMed:11517327}. |
| Top Pathways | RNA transport |
PIMT CRISPR/Cas9 KO Plasmid (h) - sc-407101 from Santa Cruz Biotechnology
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PIMT HDR Plasmid (h) - sc-407101-HDR from Santa Cruz Biotechnology
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PIMT Double Nickase Plasmid (h) - sc-407101-NIC from Santa Cruz Biotechnology
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PIMT Double Nickase Plasmid (h2) - sc-407101-NIC-2 from Santa Cruz Biotechnology
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PIMT CRISPR Activation Plasmid (h) - sc-407101-ACT from Santa Cruz Biotechnology
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PIMT CRISPR Activation Plasmid (h2) - sc-407101-ACT-2 from Santa Cruz Biotechnology
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PIMT Lentiviral Activation Particles (h) - sc-407101-LAC from Santa Cruz Biotechnology
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PIMT Lentiviral Activation Particles (h2) - sc-407101-LAC-2 from Santa Cruz Biotechnology
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PIMT siRNA (h) - sc-45875 from Santa Cruz Biotechnology
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PIMT shRNA Plasmid (h) - sc-45875-SH from Santa Cruz Biotechnology
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PIMT shRNA (h) Lentiviral Particles - sc-45875-V from Santa Cruz Biotechnology
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PIMT RNAi - H00096764-R01 from Novus Biologicals
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MISSION® esiRNA - EHU003091 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN21288 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND21288 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR06318 from Sigma-Aldrich
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TGS1 siRNA (Human) - CRJ4269 from Cohesion Biosciences
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TGS1 - MBS8235889 from MyBioSource
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PIMT CRISPR/Cas9 KO Plasmid (h2) - sc-407101-KO-2 from Santa Cruz Biotechnology
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PIMT HDR Plasmid (h2) - sc-407101-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human TGS1 (NM_024831.6) - SHH426608 from Creative biogene
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shRNA set against Human TGS1(NM_024831.6) - SHL042364 from Creative biogene
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Human TGS1 siRNA - orb274770 from Biorbyt
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TGS1 shRNA Plasmid (Human) - CHJ4269 from Cohesion Biosciences
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TGS1 miRNA 3'UTR clone - MiUTR1H-10365 from Creative biogene
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