TGS1

Gene Symbol TGS1
Entrez Gene 96764
Alt Symbol NCOA6IP, PIMT, PIPMT
Species Human
Gene Type protein-coding
Description trimethylguanosine synthase 1
Other Description CLL-associated antigen KW-2|PRIP-interacting protein PIPMT|PRIP-interacting protein with methyltransferase domain|PRIP-interacting protein with methyltransferase motif|SEREX-defined|cap-specific guanine-N2 methyltransferase|hepatocellular carcinoma-associated antigen 137|nuclear receptor coactivator 6 interacting protein|nuclear receptor coactivator 6-interacting protein|trimethylguanosine synthase|trimethylguanosine synthase homolog
Swissprots Q8TDG9 A6NJQ5 Q96QU3 Q5GH23 Q96RS0 Q9H5V3
Accessions EAW86761 EAW86762 EAW86763 Q96RS0 AF286340 AAK83025 AF432215 AAL99922 AK026648 BAB15516 AK055178 BAG51481 AK314693 BAG37244 AL832426 AY028423 AAK27730 AY534911 AAT02709 BC011999 AAH11999 BC160105 AAI60105 BM853546 BX648548 DB082992 XM_005251328 XP_005251385 XM_006716485 XP_006716548 XM_006716486 XP_006716549 NM_024831 NP_079107
Function Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation. {ECO:0000269|PubMed:11517327, ECO:0000269|PubMed:11912212, ECO:0000269|PubMed:16687569, ECO:0000269|PubMed:18775984}.
Subcellular Location Cytoplasm. Nucleus, Cajal body. Note=A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network.
Tissue Specificity Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta. {ECO:0000269|PubMed:11517327}.
Top Pathways RNA transport

PIMT (P-12) - sc-23114 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIMT (P-12): sc-23114. Western blot analysis of PIMT expression in A-10 (A), Sol8 (B), KNRK (C), NIH/3T3 (D), HeLa (E) and SW480 (F) nuclear extracts.

PIMT (N-13) - sc-23113 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIMT (N-13): sc-23113. Western blot analysis of PIMT expression in HeLa whole cell lysate.

Anti-PIMT antibody - ab70559 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling PIMT with ab70559 at 1/200 (1µg/ml). Detection: DAB.

Anti-PIMT antibody - ab121849 from Abcam

Prices $443.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab121849 at 1/250 dilution staining PIMT in Paraffin-embedded Human Kidney tissue by Immunohistochemistry. Note: strong nuclear positivity in tubular and glomerular cells.

Anti-TGS1 / PIMT Antibody (aa119-133) - LS-C17282 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-TGS1 / PIMT Antibody (aa839-853) - LS-C40678 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TGS1 / PIMT Antibody (aa150-200) - LS-C91913 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-TGS1 / PIMT Antibody (aa839-853) - LS-C91912 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TGS1 / PIMT Antibody (aa119-133) - LS-C147877 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-TGS1 / PIMT Antibody (aa408-423) - LS-C147894 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-TGS1 / PIMT Antibody (aa150-200) - LS-C287670 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PIMT by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PIMT used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-TGS1 / PIMT Antibody (aa432-481) - LS-C111206 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TGS1 antibody LS-C111206 Western blot of HepG2 cell lysate.

Anti-TGS1 / PIMT Antibody (N-Terminus) - LS-C97896 from LifeSpan Bioscience

Prices $250.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of hNCOA6IP N-term D5 LS-C97896 in NIH-3T3 cell line lysates (35 ug/lane). NCOA6IP (arrow) was detected using the purified Pab.

Anti-TGS1 / PIMT Antibody (aa150-200) - LS-C286681 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PIMT by Immunohistochemistry. Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-PIMT used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Anti-TGS1 / PIMT Antibody (C-Terminus) IHC-plus™ - LS-B255 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-TGS1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B255 concentration 5 ug/ml.

PIMT Antibody - NB100-2595 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PIMT Antibody [NB100-2595] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2595 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).

PIMT Antibody - NBP1-54942 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PIMT Antibody [NBP1-54942] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.

PIMT Antibody (3F1) - H00096764-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F1

Anti-TGS1 antibody produced in rabbit - SAB2102421 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-TGS1: <B>Cat. No.SAB2102421</B>: TGS1 in HepG2 cell lysate with TGS1 antibody at 1.0 μg/mL.

Anti-TGS1 antibody produced in rabbit - HPA025024 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-TGS1: <B>Cat. No. HPA025024</B>: Immunohistochemical staining of human kidney shows strong nuclear positivity in tubular and glomerular cells.

Monoclonal Anti-NCOA6IP antibody produced in mouse - WH0096764M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F1
<B>Sandwich ELISA</B><BR/>Monoclonal Anti-NCOA6IP, <B>Cat. No. WH0096764M1</B>: the detection limit for recombinant GST tagged NCOA6IP is approximately 0.3ng/mL.

TGS1 antibody - middle region (ARP48923_P050) - ARP48923_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NCOA6IP-Antibody-monoclonal-M01 - AT2986a from Abgent, a WuXi AppTec company

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F1
Detection limit for recombinant GST tagged NCOA6IP is approximately 0.3ng/ml as a capture antibody.

TGS1 Polyclonal Antibody - A5103 from ABclonal

Prices $200.00, $480.00
Sizes 50 µg, 200 µg
Host Rabbit
Clonality Polyclonal

PIMT antibody - 70R-PR064 from Fitzgerald

Prices $250.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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