RNF20
| Gene Symbol | RNF20 |
|---|---|
| Entrez Gene | 56254 |
| Alt Symbol | BRE1, BRE1A, hBRE1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | ring finger protein 20, E3 ubiquitin protein ligase |
| Other Description | BRE1 E3 ubiquitin ligase homolog|BRE1-A|E3 ubiquitin-protein ligase BRE1A|homolog of S. cerevisiae BRE1 |
| Swissprots | Q9HA51 Q9NUR4 Q9H9Y7 Q8N3J4 Q5VTR2 Q6P527 Q96JD3 A7MCT5 Q9NX83 Q2TB34 Q69YL5 Q9NWQ3 |
| Accessions | AAG24713 EAW58955 EAW58956 EAW58957 Q5VTR2 AF265230 AAK58539 AF283791 AAG44865 AF305515 AAG21410 AK000389 BAA91134 AK000697 BAA91326 AK002051 BAA92057 AK022300 BAB14005 AK022532 BAB14081 AK314401 BAG37025 AL832910 CAH10630 AL834272 CAD38947 AY006321 AAG15824 BC016691 AAH16691 BC032618 AAH32618 BC063115 AAH63115 BC110584 AAI10585 BC110585 AAI10586 BC152309 AAI52310 DB273543 XM_011518862 XP_011517164 NM_019592 NP_062538 |
| Function | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. {ECO:0000269|PubMed:16307923, ECO:0000269|PubMed:16337599, ECO:0000269|PubMed:19037095, ECO:0000269|PubMed:19410543}. |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:19037095, ECO:0000305|PubMed:16337599}. |
| Tissue Specificity | Expressed in the normal brain and also in malignant gliomas (at protein level). {ECO:0000269|PubMed:19037095}. |
RNF20 CRISPR/Cas9 KO Plasmid (h) - sc-412695 from Santa Cruz Biotechnology
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RNF20 HDR Plasmid (h) - sc-412695-HDR from Santa Cruz Biotechnology
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RNF20 Double Nickase Plasmid (h) - sc-412695-NIC from Santa Cruz Biotechnology
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RNF20 Double Nickase Plasmid (h2) - sc-412695-NIC-2 from Santa Cruz Biotechnology
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RNF20 CRISPR Activation Plasmid (h) - sc-412695-ACT from Santa Cruz Biotechnology
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RNF20 CRISPR Activation Plasmid (h2) - sc-412695-ACT-2 from Santa Cruz Biotechnology
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RNF20 Lentiviral Activation Particles (h) - sc-412695-LAC from Santa Cruz Biotechnology
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RNF20 Lentiviral Activation Particles (h2) - sc-412695-LAC-2 from Santa Cruz Biotechnology
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RNF20 siRNA (h) - sc-92753 from Santa Cruz Biotechnology
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RNF20 shRNA Plasmid (h) - sc-92753-SH from Santa Cruz Biotechnology
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RNF20 shRNA (h) Lentiviral Particles - sc-92753-V from Santa Cruz Biotechnology
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RNF20 RNAi - H00056254-R01 from Novus Biologicals
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MISSION® esiRNA - EHU036561 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN18415 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND18415 from Sigma-Aldrich
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RNF20 siRNA (Human) - CRJ1117 from Cohesion Biosciences
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RNF20 - MBS8228043 from MyBioSource
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shRNA set against Human RNF20 (NM_019592.5) - SHH397100 from Creative biogene
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shRNA set against Human RNF20(NM_019592.5) - SHR186508 from Creative biogene
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RNF20 shRNA Plasmid (Human) - CHJ1117 from Cohesion Biosciences
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RNF20 miRNA 3'UTR clone - MiUTR1H-08898 from Creative biogene
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