WWP2

Gene Symbol WWP2
Entrez Gene 11060
Alt Symbol AIP2, WWp2-like
Species Human
Gene Type protein-coding
Description WW domain containing E3 ubiquitin protein ligase 2
Other Description NEDD4-like E3 ubiquitin-protein ligase WWP2|atrophin-1 interacting protein 2
Swissprots H3BRF3 B2R706 O00308 Q96CZ2 Q9BWN6 F5H213 I3RSG8 B4DTL5 Q6ZTQ5 A6NEP1
Accessions EAW83287 EAW83288 EAW83289 O00308 AI494370 AI635734 AJ001982 AK126332 BAC86528 AK295070 BAG58117 AK295701 BAG58549 AK300266 BAG62027 AK312792 BAG35653 AK316097 BAH14468 AL527115 AL583085 BC000108 AAH00108 BC013645 AAH13645 BC064531 AAH64531 BQ183829 BU167468 BX372347 DB288556 DC402912 DQ891174 ABM82100 DQ894356 ABM85282 JN712744 AFK29253 U96114 AAC51325 XM_011522823 XP_011521125 XM_011522824 XP_011521126 XM_011522825 XP_011521127 XM_011522826 XP_011521128 NM_001270453 NP_001257382 NM_001270454 NP_001257383 NM_001270455 NP_001257384 NM_007014 NP_008945 NM_199424 NP_955456
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation- induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation. {ECO:0000269|PubMed:19274063, ECO:0000269|PubMed:19651900}.
Subcellular Location Nucleus {ECO:0000269|PubMed:19274063, ECO:0000269|PubMed:20858735}.
Tissue Specificity Detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney and pancreas. Also detected in spleen and peripheral blood leukocytes. {ECO:0000269|PubMed:19274063, ECO:0000269|PubMed:9647693}.
Top Pathways Ubiquitin mediated proteolysis

AIP2 (A-3) - sc-398090 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AIP2 (A-3): sc-398090. Western blot analysis of AIP2 expression in non-transfected 293T: sc-117752 (A), human AIP2 transfected 293T: sc-116898 (B), K-562 (C), Jurkat (D), HeLa (E) and NIH/3T3 (F) whole cell lysates.

AIP2 (A-5) - sc-166239 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AIP2 (A-5): sc-166239. Western blot analysis of AIP2 expression in non-transfected: sc-117752 (A) and human AIP2 transfected: sc-116898 (B) 293T whole cell lysates.

AIP2 (G-9) - sc-166240 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AIP2 (G-9): sc-166240. Western blot analysis of AIP2 expression in non-transfected: sc-117752 (A) and human AIP2 transfected: sc-116898 (B) 293T whole cell lysates.

AIP2 (H-300) - sc-30052 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
AIP2 (H-300): sc-30052. Western blot analysis of AIP2 expression in non-transfected: sc-110760 (A) and human AIP2 transfected: sc-112272 (B) 293 whole cell lysates.

AIP2 (N-15) - sc-11896 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AIP2 (N-15): sc-11896. Western blot analysis of AIP2 expression in non-transfected: sc-110760 (A) and human AIP2 transfected: sc-112272 (B) 293 whole cell lysates.

AIP2 (V-20) - sc-11897 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AIP2 (V-20): sc-11897. Western blot analysis of AIP2 expression in non-transfected: sc-117752 (A) and human AIP2 transfected: sc-116898 (B) 293T whole cell lysates.

Anti-Wwp2 antibody - ab103527 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Wwp2 antibody (ab103527) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T at 50 µgLane 5 : NIH3T3 at 50 µgdeveloped using the ECL technique

Anti-WWP2 Antibody (aa200-250) - LS-C289643 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-WWP2 Antibody (aa150-200) - LS-C289644 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-WWP2 Antibody (C-Terminus) - LS-C80543 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-WWP2 Antibody (N-Terminus) - LS-C40407 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
WWP2 antibody LS-C40407 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-WWP2 Antibody (aa395-444) - LS-C110756 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
WWP2 antibody LS-C110756 Western blot of Transfected 293T cell lysate.

Anti-WWP2 Antibody (aa323-372) - LS-C101998 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
WWP2 antibody LS-C101998 Western blot of Transfected 293T cell lysate.

Anti-WWP2 Antibody - LS-C332087 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of A549 cell line, using WWP2 antibody.

WWP2 Antibody - NBP1-53089 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: WWP2 Antibody [NBP1-53089] - Titration: 0.2-1 ug/ml, Positive Control: Transfected 293T.

WWP2 Antibody - NBP1-49942 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: WWP2 Antibody [NBP1-49942] - Detection of Human and Mouse WWP2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-WWP2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. WWP2 was also immunoprecipitated by rabbit anti-WWP2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated WWP2 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 second (B).

WWP2 Antibody - NBP2-13526 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: WWP2 Antibody [NBP2-13526] - Staining of human heart muscle shows distinct cytoplasmic positivity in myocytes.

WWP2 Antibody - H00011060-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: WWP2 Antibody [H00011060-D01P] - analysis of WWP2 expression in transfected 293T cell line by WWP2 MaxPab rabbit polyclonal antibody. Lane 1: WWP2 transfected lysate (35.20 KDa). Lane 2: Non-transfected lysate.

WWP2 Antibody - H00011060-A01 from Novus Biologicals

Host Mouse
Clonality Polyclonal

Anti-WWP2 antibody produced in rabbit - SAB2103031 from Sigma-Aldrich

Prices $347.50
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-WWP2, antibody produced in rabbit: <B>Cat. No. SAB2103031</B>: Immunoblotting analysis of WWP2 in transfected 293T cell lysate with WWP2 anitbody at 1 μg/mL.

Anti-WWP2 antibody produced in mouse - SAB1407097 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-WWP2: <B>Cat. No. SAB1407097</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~36.9 kDa using WWP2 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-WWP2 (AB1) antibody produced in rabbit - AV43088 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-WWP2 (ab1): <B>Cat. No. AV43088</B>: Immunohistochemistry of WWP2 in Human Kidney tissue with WWP2 antibody at 4-8 μg/mL.

Anti-WWP2 antibody produced in rabbit - HPA041682 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-WWP2:<B>Cat. No. HPA041682</B>: Immunohistochemical staining of human heart muscle shows distinct cytoplasmic positivity in myocytes.

Anti-WWP2 (AB2) antibody produced in rabbit - AV43089 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: 721_B (<B>Cat. No. AV43089</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1 μg/mL in cell type 721_B

Anti-WWP2 (338-350) antibody produced in rabbit - W1644 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-WWP2 (338-350), <B>Cat. No. W1644</B>: Immunofluorescence of HUVEC cells using Anti-WWP2 (338-350), W1644 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
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