Raf1

Gene Symbol Raf1
Entrez Gene 24703
Alt Symbol -
Species Rat
Gene Type protein-coding
Description v-raf-leukemia viral oncogene 1
Other Description C-RAF|RAF proto-oncogene serine/threonine-protein kinase|cRaf|murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV)|proto-oncogene c-RAF|raf-1|v-raf-1 murine leukemia viral oncogene homolog 1
Swissprots P11345
Accessions EDM02147 EDM02148 EDM02149 P11345 BC062071 AAH62071 M15427 AAA42001 XM_008763217 XP_008761439 XM_008763218 XP_008761440 NM_012639 NP_036771
Function Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/M
Subcellular Location Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Mitochondrion {ECO:0000250}. Nucleus {ECO:0000250}. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus (By similarity). {ECO:0000250}.
Top Pathways VEGF signaling pathway, Focal adhesion, Gap junction, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway

Raf-1 [p Ser301] Antibody - NB300-305 from Novus Biologicals

Prices $279.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Raf1 [p Ser301] Antibody [NB300-305] - UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf-1 protein (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Ser301< Raf-1. The immunolabeling of Raf-1 is completely eliminated by treatment with lambda Ptase.

Raf-1 [p Ser642] Antibody - NB300-306 from Novus Biologicals

Prices $279.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Raf1 antibody produced in rabbit - SAB4300639 from Sigma-Aldrich

Prices $397.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-Raf1 <B>Cat. No. SAB4300639</B>: Analysis of extract from mouse brain tissue and C6 cells using Anti-Raf1 antibody.

Phospho-Ser642-Raf-1-Antibody - AN1095 from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Phospho-Ser301-Raf-1-Antibody - AN1084 from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the~74k Raf-1 protein (Control). The phosphospecificity of this labeling is shown in the second lane(lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubatedin λ-Ptase (1200 units for 30 min) before being exposed to the phospho Ser301 Raf-1 antibody.The immunolabeling of Raf-1 is completely eliminated by treatment with λ-Ptase.

RAF1 (phospho-Ser43) antibody - orb34589 from Biorbyt

Prices $187.00, $383.00
Sizes 80 μl (Trial size), 400 μl
Host Rabbit
Clonality Polyclonal

RAF1 (phospho-Ser338) antibody - orb34606 from Biorbyt

Prices $60.00, $187.00, $383.00
Sizes 5 μg (Trial size), 80 μl (Trial size), 400 μl
Host Rabbit
Clonality Polyclonal
Western blot analysis of Phospho-RAF1-pS338 antibody in human TPA activated Hela cell line lysates

RAF1 pSer301 antibody - AP08734PU-N from Acris Antibodies

Prices $350.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Figure 1. Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf-1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-Phosphatase). The blot is identical to th

RAF1 pSer642 antibody - AP08735PU-N from Acris Antibodies

Prices $350.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RAF1 pSer301 antibody - PAB9647 from Acris Antibodies

Prices $500.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Raf1 (phospho S301) polyclonal antibody ( Cat # PAB9647 ). The immunolabeling of Raf1 is completely eliminated by treatment with l-Ptase.

RAF1 pSer642 antibody - PAB9660 from Acris Antibodies

Prices $500.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Raf 1 Antibody - 79-697 from ProSci

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extract from mouse brain tissue and C6 cells using Raf-1 Antibody.

Raf-1 (phospho Ser301) Antibody - 50-237 from ProSci

Prices $350.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf-1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho Ser301 Raf-1 antibody. The immunola

Raf-1 (phospho Ser642) Antibody - 50-238 from ProSci

Prices $350.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Raf-1 (Ser642) Antibody - 252579 from ABBIOTEC

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Raf-1 (Ser301) Antibody - 252578 from ABBIOTEC

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

BRAF (1H12) Antibody - 252864 from ABBIOTEC

Prices $356.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H12
Western blot analysis using BRAF (1H12) Antibody (Cat. No. 252864) against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysates.

BRAF (1F12) Antibody - 252685 from ABBIOTEC

Prices $436.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H12F1, 1H12G10, 1F12F11C9
Western blot analysis using BRAF (1F12) Antibody (Cat. No. 252640) against truncated recombinant Braf (1) and A431 cell lysate (2).

RAF-1 Antibody (phospho Ser642) - GWB-9A4E77 from Genway Biotech

Prices $849.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RKIP - MBS607211 from MyBioSource

Clonality Monoclonal
Clone 9E513

Rabbit Anti-RAF1(Pser642) Polyclonal antibody - CPBT-66568RR from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Phospho-c-Raf (Ser642) Polyclonal Antibody - PA1-4640 from Thermo Fisher Scientific

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RAF-1 , Polyclonal Antibody - MBS221524 from MyBioSource

Prices $705.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Raf-1 (Ser301) , Polyclonal Antibody - MBS502110 from MyBioSource

Prices $345.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Raf-1 (Ser642) , Polyclonal Antibody - MBS502111 from MyBioSource

Prices $345.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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