E2F8

Gene Symbol E2F8
Entrez Gene 79733
Alt Symbol E2F-8
Species Human
Gene Type protein-coding
Description E2F transcription factor 8
Other Description E2F family member 8|transcription factor E2F8
Swissprots Q9H5M0 Q3C1U6 Q5BKY4 Q2VPJ3 A8K9H3 A0AVK6 Q8N340
Accessions A0AVK6 EAW68354 AB231781 BAE46901 AB231782 AB231783 AK026964 BAB15605 AK055206 AK225338 AK292688 BAF85377 BC028244 AAH28244 BC090877 AAH90877 BC108700 AAI08701 BC126400 AAI26401 BC126402 AAI26403 BM972658 BU164108 BX504614 CB959621 DA758300 HQ258679 ADR83430 XM_011520367 XP_011518669 XM_011520368 XP_011518670 XR_930907 NM_001256371 NP_001243300 NM_001256372 NP_001243301 NM_024680 NP_078956
Function Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different
Subcellular Location Nucleus {ECO:0000269|PubMed:15897886}.

E2F-8 (B-9) - sc-514064 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
E2F-8 (B-9): sc-514064. Western blot analysis of E2F-8 expression in non-transfected 293: sc-110760 (A) and human E2F-8 transfected 293: sc-114760 (B) whole cell lysates and HeLa nuclear extract (C).

E2F-8 (9) - sc-130313 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
E2F-8 (9): sc-130313. Western blot analysis of E2F-8 expression in non-transfected: sc-110760 (A) and truncated human E2F-8 transfected: sc-114760 (B) 293 whole cell lysates.

E2F-8 (C-15) - sc-161538 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

E2F-8 (S-14) - sc-161540 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-E2F8 antibody - ab109596 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-E2F8 antibody (ab109596) at 0.1 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 5 µgLane 4 : HeLa whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-E2F8 antibody - ab57775 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57775 E2F8 antibody at 1ug/ml.

Anti-E2F8 antibody - ab126163 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-E2F8 antibody (ab126163) at 1/500 dilutionLane 1 : A431 whole cell lysateLane 2 : HepG2 whole cell lysate Lysates/proteins at 30 µg per lane.

Anti-E2F8 Antibody (aa402-416) - LS-C112778 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-E2F8 Antibody (clone 3E9-2F5) - LS-C307337 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9-2F5

Anti-E2F8 Antibody (C-Terminus) - LS-C345637 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of E2F8 Antibody with human RPMI-8226 Whole Cell lysate.

Anti-E2F8 Antibody (C-Terminus) - LS-C107893 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
E2F8 antibody LS-C107893 Western blot of COLO205 cell lysate.

Anti-E2F8 Antibody (aa817-867) - LS-C286232 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse E2F8 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (5, 15, and 50 ug for WB) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-E2F8 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. E2F8 was also immunoprecipitated by rabbit anti-E2F8 antibody BL11013, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Anti-E2F8 Antibody (N-Terminus) - LS-C201541 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human PANC1. E2F8 antibody dilution 1.0 ug/ml.

Anti-E2F8 Antibody - LS-C192386 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of E2F8 pAb in extracts from Hela cells.

Anti-E2F8 Antibody - LS-C331285 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of SW480 cell line, using E2F8 antibody.

E2F8 Antibody - NBP1-52650 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: E2F8 Antibody [NBP1-52650] - Whole cell lysate from HeLa (H; 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (5, 15, and 50 mcg for WB) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-E2F8 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. E2F8 was also immunoprecipitated by another rabbit anti-E2F8 antibody, which recognizes an upstream epitope.

E2F8 Antibody - NBP1-36952 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

E2F8 Antibody - NBP2-16252 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: E2F8 Antibody [NBP2-16252] - Sample (30 ug of whole cell lysate) A: A431 B: HepG2 5% SDS PAGE gel, diluted at 1:500.

E2F8 Antibody (3E9-2F5) - H00079733-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9-2F5
Western Blot: E2F8 Antibody (3E9-2F5) [H00079733-M01] - Western Blot detection against Immunogen (73.15 KDa).

Monoclonal Anti-E2F8 antibody produced in mouse - WH0079733M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9-2F5
<B>Immunoblotting</B><BR/>Monoclonal Anti-E2F8, <B>Cat. No. WH0079733M1</B> used at the antibody concentration: 1 μg/mL. Specific band of ~73.52 kDa using immunogen protein lysate.

Anti-E2F8 antibody produced in rabbit - SAB2701122 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-E2F8: <B>Cat. No. SAB2701122</B>: Western Blot analysis of E2F8 in extracts from <B>Lanes</B><BR/>A. A431 <BR/>B. HepG2 using <B>Cat. No. SAB2701122</B> at a 1:500 dilution.

Anti-E2F8 antibody produced in rabbit - SAB2105094 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-E2F8, antibody produced in rabbit: <B>Cat. No. SAB2105094</B>: Immunoblotting analysis of E2F8 in COLO205 cell lysate with E2F8 anitbody at 1.0μg/mL.

E2F8 Antibody - N-terminal region (ARP47683_P050) - ARP47683_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

E2F8 antibody - middle region (ARP47684_P050) - ARP47684_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

E2F8 Antibody - C-terminal region (ARP73834_P050) - ARP73834_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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