MGAT4A
| Gene Symbol | MGAT4A |
|---|---|
| Entrez Gene | 11320 |
| Alt Symbol | GNT-IV, GNT-IVA, GnT-4a |
| Species | Human |
| Gene Type | protein-coding |
| Description | mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A |
| Other Description | N-acetylglucosaminyltransferase IVa|N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa|UDP-GlcNAc:a-1,3-D-mannoside b-1,4-acetylglucosaminyltransferase IV|UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa|UDP-N-acetylglucosamine:alpha1,3-d-mannoside beta1,4-N-acetylglucosaminyltransferase|alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A|alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase|glcNAc-T IVa|mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isoenzyme A |
| Swissprots | Q53S97 D3DVH6 Q9UM21 Q86Z15 B4E2R6 E9PEN2 |
| Accessions | AAY14976 AAY24092 BAC55023 EAX01896 EAX01897 EAX01898 Q9UM21 AB000616 BAA86388 AK092952 AK128366 AK291533 BAF84222 AK304393 BAG65228 AK309852 AK314024 AW138839 BC127107 AAI27108 BC127108 AAI27109 NM_001160154 NP_001153626 NM_012214 NP_036346 |
| Function | Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N- linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells. |
| Subcellular Location | Alpha-1,3-mannosyl-glycoprotein 4-beta-N- acetylglucosaminyltransferase A: Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. |
| Tissue Specificity | Expressed in pancreas, spleen, thymus, prostate, small intestine, peripheral blood leukocytes and lymph node. Strongly overexpressed in choriocarcinoma cancer cell lines. Down-regulated in pancreatic cancer. {ECO:0000269|PubMed:10024668}. |
| Top Pathways | N-Glycan biosynthesis |
GnT-IVA CRISPR/Cas9 KO Plasmid (h) - sc-407146 from Santa Cruz Biotechnology
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GnT-IVA HDR Plasmid (h) - sc-407146-HDR from Santa Cruz Biotechnology
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GnT-IVA Double Nickase Plasmid (h) - sc-407146-NIC from Santa Cruz Biotechnology
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GnT-IVA Double Nickase Plasmid (h2) - sc-407146-NIC-2 from Santa Cruz Biotechnology
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GnT-IVA CRISPR Activation Plasmid (h) - sc-407146-ACT from Santa Cruz Biotechnology
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GnT-IVA CRISPR Activation Plasmid (h2) - sc-407146-ACT-2 from Santa Cruz Biotechnology
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GnT-IVA Lentiviral Activation Particles (h) - sc-407146-LAC from Santa Cruz Biotechnology
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GnT-IVA Lentiviral Activation Particles (h2) - sc-407146-LAC-2 from Santa Cruz Biotechnology
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GnT-IVA siRNA (h) - sc-94905 from Santa Cruz Biotechnology
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GnT-IVA shRNA Plasmid (h) - sc-94905-SH from Santa Cruz Biotechnology
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GnT-IVA shRNA (h) Lentiviral Particles - sc-94905-V from Santa Cruz Biotechnology
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MGAT4A RNAi - H00011320-R01 from Novus Biologicals
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MISSION® esiRNA - EHU132161 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN13275 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND13275 from Sigma-Aldrich
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MGAT4A siRNA (Human) - CRH7750 from Cohesion Biosciences
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shRNA set against Human MGAT4A (NM_012214.2) - SHH341375 from Creative biogene
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shRNA set against Human MGAT4A(NM_012214.2) - SHH187323 from Creative biogene
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Human MGAT4A siRNA - orb268252 from Biorbyt
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MGAT4A shRNA Plasmid (Human) - CHH7750 from Cohesion Biosciences
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MGAT4A miRNA 3'UTR clone - MiUTR1H-06244 from Creative biogene
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