POFUT1

Gene Symbol POFUT1
Entrez Gene 23509
Alt Symbol DDD2, FUT12, O-FUT, O-Fuc-T, O-FucT-1, OFUCT1
Species Human
Gene Type protein-coding
Description protein O-fucosyltransferase 1
Other Description GDP-fucose protein O-fucosyltransferase 1|o-fucosyltransferase protein|peptide-O-fucosyltransferase 1
Swissprots Q5W185 Q9BW76 Q9H488 Q14685 E1P5M4 A8K4R8
Accessions EAW76383 EAW76384 EAW76385 Q9H488 AF375884 AAL09576 AK291033 BAF83722 AK300810 BAG62466 AK303068 BAG64185 AL832023 BC000582 AAH00582 BC017669 D80002 BAA11497 XR_430297 NM_015352 NP_056167 NM_172236 NP_758436
Function Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs). {ECO:0000269|PubMed:11524432, ECO:0000269|PubMed:8358148}.
Subcellular Location Endoplasmic reticulum {ECO:0000250}.
Tissue Specificity Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. {ECO:0000269|PubMed:11524432}.
Top Pathways Other types of O-glycan biosynthesis

POFUT1 (H-280) - sc-98435 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
POFUT1 (H-280): sc-98435. Western blot analysis of POFUT1 expression in non-transfected: sc-117752 (A) and mouse POFUT1 transfected: sc-127362 (B) 293T whole cell lysates.

POFUT1 (F-7) - sc-271026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
POFUT1 (F-7): sc-271026. Western blot analysis of POFUT1 expression in Hep G2 (A) and JAR (B) whole cell lysates.

POFUT1 (N-20) - sc-79694 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

POFUT1 (E-20) - sc-79693 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-Pofut1 antibody - ab74302 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.

Anti-POFUT1 Antibody (aa344-373, Biotin) - LS-C266682 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POFUT1 Antibody (aa344-373, APC) - LS-C266681 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POFUT1 Antibody (aa344-373, AP) - LS-C266680 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POFUT1 Antibody (aa344-373, Azide-free, HRP) - LS-C266684 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POFUT1 Antibody (aa344-373, FITC) - LS-C266683 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POFUT1 Antibody (aa344-373, PE) - LS-C266686 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POFUT1 Antibody (aa351-381) - LS-C324154 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POFUT1 Antibody (aa101-150) - LS-C135521 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
POFUT1 antibody LS-C135521 Western blot of PANC1 cell lysate.

Anti-POFUT1 Antibody (N-Terminus) - LS-C83909 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Western blot of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml.

Anti-POFUT1 Antibody (aa331-380) - LS-C120002 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human lung carcinoma tissue, using POFUT1 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-POFUT1 Antibody (Internal) - LS-C186579 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
POFUT1 antibody (2 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-POFUT1 Antibody (aa17-180) - LS-C179093 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-POFUT1 Antibody (aa344-373) - LS-C163834 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
POFUT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human heart tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Protein O-Fucosyltransferase 1/POFUT1 Antibody - NBP2-19882 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: POFUT1 Antibody [NBP2-19882] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.

Protein O-Fucosyltransferase 1/POFUT1 Antibody - NBP1-76940 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP1-76940] - Human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml.

Protein O-Fucosyltransferase 1/POFUT1 Antibody - NBP1-56036 from Novus Biologicals

Prices $229.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: POFUT1 Antibody [NBP1-56036] - Analysis of extracts from Jurkat/HeLa/HepG2 cells, using POFUT1 Antibody. The lane on the right is treated with the synthesized peptide.

Protein O-Fucosyltransferase 1/POFUT1 Antibody - H00023509-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [H00023509-D01P] - Analysis of POFUT1 expression in mouse kidney.

Protein O-Fucosyltransferase 1/POFUT1 Antibody (3H1) - H00023509-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3H1

Anti-POFUT1, C-Terminal antibody produced in rabbit - SAB4500991 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-POFUT1, C-Terminal: <B>Cat. No. SAB4500991</B>: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-POFUT1, C-Terminal antibody. Cells on the left were treated with the Anti-POFUT1, C-Terminal antibody. Cells on the right (negative control) were treated with both Anti-POFUT1, C-Terminal antibody and the synthesized immunogen peptide.

Anti-POFUT1 antibody produced in rabbit - PRS4765 from Sigma-Aldrich

Prices $347.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-POFUT1: <B>Cat. No. PRS4765</B> Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 μg/mL.
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