POFUT1
| Gene Symbol | POFUT1 |
|---|---|
| Entrez Gene | 23509 |
| Alt Symbol | DDD2, FUT12, O-FUT, O-Fuc-T, O-FucT-1, OFUCT1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | protein O-fucosyltransferase 1 |
| Other Description | GDP-fucose protein O-fucosyltransferase 1|o-fucosyltransferase protein|peptide-O-fucosyltransferase 1 |
| Swissprots | Q5W185 Q9BW76 Q9H488 Q14685 E1P5M4 A8K4R8 |
| Accessions | EAW76383 EAW76384 EAW76385 Q9H488 AF375884 AAL09576 AK291033 BAF83722 AK300810 BAG62466 AK303068 BAG64185 AL832023 BC000582 AAH00582 BC017669 D80002 BAA11497 XR_430297 NM_015352 NP_056167 NM_172236 NP_758436 |
| Function | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs). {ECO:0000269|PubMed:11524432, ECO:0000269|PubMed:8358148}. |
| Subcellular Location | Endoplasmic reticulum {ECO:0000250}. |
| Tissue Specificity | Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. {ECO:0000269|PubMed:11524432}. |
| Top Pathways | Other types of O-glycan biosynthesis |
POFUT1 CRISPR/Cas9 KO Plasmid (h) - sc-406276 from Santa Cruz Biotechnology
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POFUT1 HDR Plasmid (h) - sc-406276-HDR from Santa Cruz Biotechnology
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POFUT1 Double Nickase Plasmid (h) - sc-406276-NIC from Santa Cruz Biotechnology
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POFUT1 Double Nickase Plasmid (h2) - sc-406276-NIC-2 from Santa Cruz Biotechnology
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POFUT1 CRISPR Activation Plasmid (h) - sc-406276-ACT from Santa Cruz Biotechnology
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POFUT1 CRISPR Activation Plasmid (h2) - sc-406276-ACT-2 from Santa Cruz Biotechnology
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POFUT1 Lentiviral Activation Particles (h) - sc-406276-LAC from Santa Cruz Biotechnology
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POFUT1 Lentiviral Activation Particles (h2) - sc-406276-LAC-2 from Santa Cruz Biotechnology
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POFUT1 siRNA (h) - sc-76184 from Santa Cruz Biotechnology
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POFUT1 shRNA Plasmid (h) - sc-76184-SH from Santa Cruz Biotechnology
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POFUT1 shRNA (h) Lentiviral Particles - sc-76184-V from Santa Cruz Biotechnology
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Protein O-Fucosyltransferase 1/POFUT1 RNAi - H00023509-R01 from Novus Biologicals
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Protein O-Fucosyltransferase 1/POFUT1 RNAi - H00023509-R02 from Novus Biologicals
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MISSION® esiRNA - EHU091561 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17082 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17082 from Sigma-Aldrich
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POFUT1 siRNA (Human) - CRH8315 from Cohesion Biosciences
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shRNA set against Human POFUT1 (NM_172236.1) - SHH379000 from Creative biogene
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shRNA set against Human POFUT1(NM_172236.1) - SHR125840 from Creative biogene
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shRNA set against Human POFUT1(NM_015352.1) - SHR125894 from Creative biogene
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Human POFUT1 siRNA - orb268817 from Biorbyt
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POFUT1 shRNA Plasmid (Human) - CHH8315 from Cohesion Biosciences
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POFUT1 miRNA 3'UTR clone - MiUTR1H-08055 from Creative biogene
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POFUT1 miRNA 3'UTR clone - MiUTR1H-08056 from Creative biogene
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