| Gene Symbol |
TLR9
|
| Entrez Gene |
54106
|
| Alt Symbol |
CD289
|
| Species |
Human
|
| Gene Type |
protein-coding
|
| Description |
toll-like receptor 9
|
| Other Description |
-
|
| Swissprots |
Q9HD70 Q9NYC3 B3Y661 Q9NYC2 D1CS56 Q9HD69 Q6UVZ2 Q9NR96 Q9HD68
|
| Accessions |
AAZ95513 AAZ95514 AAZ95515 AAZ95516 AAZ95517 AAZ95518 AAZ95519 AAZ95520 ABW37074 ABW37075 ABW37076 ABW37077 ABW37078 ACQ41818 ACQ41819 ACQ41820 ACQ41821 ACQ41822 ACQ41823 ACQ41824 ACQ41825 ACQ41826 ACQ41827 ACQ41828 ACQ41829 ACQ41830 ACQ41831 CCQ43550 EAW65191 EAW65193 Q9NR96 AB045180 BAB19259 AB445673 BAG55070 AF245704 AAF78037 AF246972 AAG01734 AF259262 AAF72189 AF259263 AAF72190 AY359085 AAQ89443 BC032713 AAH32713 EU831821 ACE87339 EU831898 ACE86657 NM_017442 NP_059138 NM_138688
|
| Function |
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. {ECO:0000269|PubMed:11564765, ECO:0000269|PubMed:17932028}.
|
| Subcellular Location |
Endoplasmic reticulum membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}. Endosome {ECO:0000250}. Lysosome {ECO:0000250}. Cytoplasmic vesicle, phagosome {ECO:0000250}. Note=Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. {ECO:0000250}.
|
| Tissue Specificity |
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
|
| Top Pathways |
Toll-like receptor signaling pathway, Chagas disease (American trypanosomiasis), Malaria, African trypanosomiasis, Measles
|
![Western Blot: TLR9 Overexpression Lysate (Adult Normal) [NBL1-16957] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TLR9.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/9443_TLR9%2520Overexpression%2520Lysate%2520(Adult%2520Normal)-Western%2520Blot-NBL1-16957-img0002.jpg)
![Ligand Activation: Human TLR9 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26280] - LR9/NF-kB SEAPorterTM HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of CpG for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. CpG-mediated TLR9 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of CpG. The EC50, or the half maximal activity concentration, represents the concentration of CpG that was required for 50% activation of TLR9 as measured by SEAP activity. Data Summary: CpG activated the cell line in a dose response manner, of which EC50 was measured as 4.401 ug/ml.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/10745_Human-TLR9-NF-kB-SEAP---(SEAPorter(TM))-Stable-Reporter-Cell-Line-Ligand-Activation-NBP2-26280-img0003.jpg)
![Flow Cytometry: Human TLR9 Stable Cell Line [NBP2-26272] - analysis of the TLR9 stable cell line. The assay was performed using the NF-kB SEAPorter™ Assay Kit. The Vector control stable cell line and TLR9 stable cell line were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/10752_Human%2520TLR9%2520Stable%2520Cell%2520Line-Flow%2520Cytometry-NBP2-26272-img0004.jpg)