H1FNT

Gene Symbol	H1FNT
Entrez Gene	341567
Alt Symbol	H1.7, H1T2
Species	Human
Gene Type	protein-coding
Description	H1 histone family, member N, testis-specific
Other Description	HANP1\|haploid germ cell-specific nuclear protein 1\|histone H1t2\|testis-specific H1 histone
Swissprots	Q147U8 Q75WM6 Q7Z694 Q5GKZ5
Accessions	AAS49492 Q75WM6 AB121028 BAD13382 AY302593 AAP60022 BC118635 AAI18636 BC119787 AAI19788 NM_181788 NP_861453
Function	Essential for normal spermatogenesis and male fertility. Required for proper cell restructuring and DNA condensation during the elongation phase of spermiogenesis. Involved in the histone- protamine transition of sperm chromatin and the subsequent production of functional sperm. Binds both double-stranded and single-stranded DNA, ATP and protamine-1 (By similarity). {ECO:0000250}.
Subcellular Location	Nucleus {ECO:0000250}. Chromosome {ECO:0000250}. Note=In round and elongating spermatids, specifically localizes to a chromatin domain at the apical pole. {ECO:0000250}.
Tissue Specificity	Testis-specific. {ECO:0000269\|PubMed:16533358}.

H1T2 (Q-12) - sc-136701 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

Anti-Histone H1t2 antibody - ab127045 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Histone H1t2 antibody (ab127045) at 1/2000 dilution + MCF7 whole cell lysate at 30 µg

Anti-Histone H1t2 antibody - N-terminal - ab184838 from Abcam

Prices	$443.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling H1FNT with ab184838 at 1/1000.

Anti-H1FNT Antibody (aa204-253) - LS-C205440 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

H1FNT antibody Western Blot of Esophagus Tumor. Antibody dilution: 1 ug/ml.

Histone H1t2 Antibody - NBP2-30774 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry: Histone H1t2 Antibody [NBP2-30774] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts

Anti-H1FNT (6-20) antibody produced in rabbit - SAB1103790 from Sigma-Aldrich

Prices	$298.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B> <BR/>Lysate of HEK-293T cells overexpressing human H1FNT was separated on SDS-PAGE, blotted with Anti-H1FNT (6-20) (<B>Cat. No. SAB1103790</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B> <BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:1,000 + H1FNT immunizing peptide (human, 6-20)

Anti-H1FNT (226-240) antibody produced in rabbit - SAB1103791 from Sigma-Aldrich

Prices	$298.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B> <BR/>Lysate of HEK-293T cells overexpressing human H1FNT was separated on SDS-PAGE, blotted with Anti-H1FNT (226-240) (<B>Cat. No. SAB1103791</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B> <BR/>1. Antibody dilution 1:1,000

Anti-H1FNT antibody produced in rabbit - SAB2701321 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-H1FNT: <B>Cat. No. SAB2701321</B>: Western Blot analysis of H1FNT in extracts from <B>Lanes</B><BR/>A. MCF-7 using <B>Cat. No. SAB2701321</B> at a 1:2000 dilution.

Anti-H1FNT antibody produced in rabbit - HPA046204 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-H1FNT: <B>Cat. No. HPA046204</B>: Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts

H1FNT Antibody - C-terminal region (ARP58274_P050) - ARP58274_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-H1FNT (aa 1-94) polyclonal antibody - DPABH-14666 from Creative Diagnostics

Host	Rabbit
Clonality	Polyclonal

Anti-H1FNT (aa 1-164) polyclonal antibody - DPABH-23122 from Creative Diagnostics

Host	Rabbit
Clonality	Polyclonal

H1FNT Polyclonal Antibody - PA5-34857 from Thermo Fisher Scientific

Prices	$349.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of Testis-specific H1 histone using 30µg of MCF-7 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Testis-specific H1 histone polyclonal antibody (Product # PA5-34857) at a dilution of 1:2000.

Testis-specific H1 histone - MBS275446 from MyBioSource

Prices	$360.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:2000

H1FNT-Antibody-C-terminal-region-AI14273 - AI14273 from Abgent, a WuXi AppTec company

Prices	$293.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Host: Rabbit Target Name: H1FNT Sample Tissue: Esophagus Tumor lysates Antibody Dilution: 1.0ug/ml

H1FNT-Antibody-N-Term - AP21787a from Abgent, a WuXi AppTec company

Prices	$99.00, $295.00
Sizes	50 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-H1FNT Antibody (N-Term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

H1FNT , Polyclonal Antibody - MBS9216525 from MyBioSource

Prices	$155.00, $340.00
Sizes	50 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-H1FNT Antibody (aa59-93) - LS-C392904 from LifeSpan Bioscience

Prices	$205.00, $305.00
Sizes	50 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-H1FNT Antibody (N-Term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

H1FNT / Histone H1t2 antibody - TA344392 from Acris Antibodies

Prices	$325.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

H1FNT antibody - orb184013 from Biorbyt

Prices	$255.00, $340.00
Sizes	100 μg, 200 μg
Host	Rabbit
Clonality	Polyclonal

Anti-H1FNT Antibody (C-Terminus, Biotin) - LS-C464803 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-H1FNT Antibody (C-Terminus, HRP) - LS-C464805 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-H1FNT Antibody (C-Terminus, FITC) - LS-C464804 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

H1FNT antibody - orb349343 from Biorbyt

Prices	$238.00, $476.00
Sizes	50 μl, 200 μl
Host	Rabbit
Clonality	Polyclonal

WB analysis of MCF-7 whole cell lysate using H1FNT antibody (dilution at 1:2000)

Anti-H1FNT Antibody A13834 - A13834 from Boster Bio

Prices	$299.00
Sizes	1
Host	Rabbit
Clonality	Polyclonal

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