GRK4

Gene Symbol GRK4
Entrez Gene 2868
Alt Symbol GPRK2L, GPRK4, GRK4a, IT11
Species Human
Gene Type protein-coding
Description G protein-coupled receptor kinase 4
Other Description ITI1
Swissprots Q53EQ8 Q14453 Q15315 Q14725 Q13294 O00642 Q15314 Q13293 Q17RH6 Q15313 Q13295 P32298 O00641 Q15316
Accessions AAB04045 AAC50409 AAC50410 AAC50411 AAC50412 CAA92341 EAW82479 EAW82480 EAW82481 EAW82482 EAW82483 EAW82484 EAW82485 P32298 AK223581 BAD97301 AK292390 BAF85079 AK293569 BAG57040 BC027597 BC039732 BC117320 AAI17321 BI465220 BM725978 HQ258463 ADR83217 U33054 AAC50406 U33055 AAC50407 U33056 AAC50408 X75897 CAA53506 X97879 CAA66468 X97880 CAA66469 X97881 CAA66470 X98118 CAA66802 XM_005247962 XP_005248019 XM_006713880 XP_006713943 XM_011513447 XP_011511749 XM_011513448 XP_011511750 XM_011513449 XP_011511751 XM_011513450 XP_011511752 XM_011513451 XP_011511753 XM_011513452 XP_011511754 XM_011513453 XP_011511755 XM_011513454 XP_011511756 XM_011513455 XP_011511757 XM_011513456 XP_011511758 XM_011513457 XP_011511759 XR_924941 XR_924943 NM_001004056 NP_001004056 NM_001004057 NP_001004057 NM_005307 NP_005298 NM_182982 NP_892027
Function Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. {ECO:0000269|PubMed:19520868, ECO:0000269|PubMed:8626439}.
Subcellular Location Cytoplasm. Cytoplasm, cell cortex. Note=Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where colocalization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 colocalize in lipid rafts of renal proximal tubule cells.
Tissue Specificity Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium. {ECO:0000269|PubMed:16636192, ECO:0000269|PubMed:8626439}.
Top Pathways Endocytosis, Chemokine signaling pathway, Morphine addiction

GRK 4 (D-11) - sc-376891 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GRK 4 (D-11): sc-376891. Western blot analysis of GRK 4 expression in BJAB (A), F9 (B) and RAW 264.7 (C) whole cell lysates.

GRK 4 (A-5) - sc-9985 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GRK 4 (A-5): sc-9985. Western blot analysis of GRK 4 expression in RAW 264.7 whole cell lysate.

GRK 4 (H-70) - sc-13079 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GRK 4 (H-70): sc-13079. Western blot analysis of GRK 4 expression in rat testis tissue extract.

GRK 4 (I-20) - sc-564 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GRK 4 (I-20): sc-564. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.

GRK 4 (K-20) - sc-1016 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GRK 4 (K-20): sc-1016. Western blot analysis of GRK 4 expression in BJAB whole cell lysate.

Anti-GRK4 antibody - ab182635 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRK4 antibody (ab182635) at 1/700 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

Anti-GRK4 antibody - ab38293 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-GRK4 antibody (ab38293) at 1/100 dilution + HL60 cell lysate

Anti-GRK4 antibody - ab55139 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab55139 GRK4 antibody at 1ug/ml.

Anti-GRK4 Antibody (clone 6D12) - LS-C197337 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D12
Immunofluorescence of monoclonal antibody to GRK4 on HeLa cell . [antibody concentration 10 ug/ml]

Anti-GRK4 Antibody (aa1-245, clone 9A3) - LS-C339827 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9A3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRK4.

Anti-GRK4 Antibody (aa359-408) - LS-C117255 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
GRK4 antibody LS-C117255 Western blot of MCF7 cell lysate.

Anti-GRK4 Antibody (aa243-292) - LS-C81746 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-GRK4/GRK5/GRK6 Antibody (aa463-590) - LS-C67122 from LifeSpan Bioscience

Prices $475.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal

GRK4 Antibody - NBP1-55313 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: GRK4 Antibody [NBP1-55313] - Jurkat cell lysate, concentration 0.2-1 ug/ml.

GRK4 Antibody - NBP2-37960 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: GRK4 Antibody [NBP2-37960] - Staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.

GRK4 Antibody (6D12) - H00002868-M01 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D12
Immunocytochemistry/Immunofluorescence: GRK4 Antibody (6D12) [H00002868-M01] - Analysis of monoclonal antibody to GRK4 on HeLa cell. Antibody concentration 10 ug/ml.

GRK4 Antibody (4D2) - H00002868-M08 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D2
ELISA: GRK4 Antibody (4D2) [H00002868-M08] - Detection limit for recombinant GST tagged GRK4 is approximately 0.3ng/ml as a capture antibody.

GRK4 Antibody - H00002868-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: GRK4 Antibody [H00002868-A01] - GRK4 polyclonal antibody (A01), Lot # 060516JCS1 Western Blot analysis of GRK4 expression in HL-60 ( Cat # L014V1 ).

GRK4 Antibody (2C5) - H00002868-M07 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C5
Sandwich ELISA: GRK4 Antibody (2C5) [H00002868-M07] - Detection limit for recombinant GST tagged GRK4 is approximately 0.1ng/ml as a capture antibody.

GRK4 Antibody (6D5) - H00002868-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D5
Sandwich ELISA: GRK4 Antibody (6D5) [H00002868-M02] - Detection limit for recombinant GST tagged GRK4 is approximately 0.1ng/ml as a capture antibody.

GRK4 Antibody (6D12) - H00002868-M01A from Novus Biologicals

Prices $349.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 6D12

GRK4+5+6 Polyclonal Antibody - bs-11235R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat brain labeled with Anti-GRK6 Polyclonal Antibody, Unconjugated (bs-2539R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

GRK4+5+6 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-11235R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GRK4+5+6 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-11235R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GRK4+5+6 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-11235R-A555 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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