AMN

Gene Symbol AMN
Entrez Gene 81693
Alt Symbol PRO1028, amnionless
Species Human
Gene Type protein-coding
Description amnion associated transmembrane protein
Other Description amnionless homolog|protein amnionless|visceral endoderm-specific type 1 transmembrane protein
Swissprots Q9BXJ7 Q6UX83
Accessions EAW81798 EAW81799 Q9BXJ7 AF328788 AAK28532 AK055802 BAG51576 AY358468 AAQ89949 BC029948 CB306421 XM_011537202 XP_011535504 XM_011537203 XP_011535505 NM_030943 NP_112205
Function Necessary for efficient absorption of vitamin B12. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm (By similarity). {ECO:0000250}.
Subcellular Location Membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.
Tissue Specificity Long isoforms are highly expressed in small intestine, colon and kidney (renal proximal tubule epithelial cells). Shorter isoforms are detected at lower levels in testis, thymus and peripheral blood leukocytes. {ECO:0000269|PubMed:12590260, ECO:0000269|PubMed:14576052}.

Amnionless (C-10) - sc-365384 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Amnionless (C-10): sc-365384. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

Amnionless (F-7) - sc-365734 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Amnionless (F-7): sc-365734. Western blot analysis of Amnionless expression in Caki-1 whole cell lysate.

Amnionless (H-280) - sc-135178 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Amnionless (H-280): sc-135178. Western blot analysis of Amnionless expression in mouse PBL whole cell lysate.

Amnionless (K-14) - sc-46726 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Amnionless (M-228) - sc-135179 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Amnionless (M-228): sc-135179. Western blot analysis of Amnionless expression in mouse PBL whole cell lysate.

Amnionless (N-15) - sc-46729 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Amnionless (N-15): sc-46729. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells.

Amnionless (N-16) - sc-46730 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-AMN antibody - ab105806 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-AMN antibody (ab105806) at 1 µg/ml + Human fetal liver lysate at 10 µg

Anti-AMN antibody [EPR12015(B)] - ab170893 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12015(B)
Western blot analysis on immunoprecipitation pellet from (1) Human fetal kidney lysate or (2) 1X PBS (negative control) using ab170893 at 1/1000 dilution. Detection utilised HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

Anti-AMN Antibody (aa35-84) - LS-C135239 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
AMN antibody LS-C135239 Western blot of Fetal Liver lysate.

Amnionless/AMN Antibody (260808) - MAB1860 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 260808

Amnionless/AMN Antibody - AF1860 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal

Amnionless/AMN Antibody - NBP1-91203 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: AMN Antibody [NBP1-91203] - Staining  of human cell line U-251MG shows positivity in nucleoli, golgi apparatus and vesicles.

Amnionless/AMN Antibody [Biotin] - BAF1860 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-AMN, (N-terminal) antibody produced in rabbit - SAB2105739 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-AMN (N-Terminal): <B>Cat. No. SAB2105739</B>: AMN in Fetal Liver cell lysates with AMN antibody at 1.0 μg/mL.

Anti-AMN antibody produced in rabbit - HPA000817 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-AMN: <B>Cat. No. HPA000817</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing strong cytoplasmic and/or membranous staining of cells in tubuli.

AMN antibody - N-terminal region (ARP49982_P050) - ARP49982_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Mouse Amnionless Antibody - AF7139 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Detection of Mouse Amnionless by Western Blot. Western blot shows recombinant mouse Amnionless (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Amnionless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7139) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Amnionless at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .

Mouse Amnionless Affinity Purified Polyclonal Ab, Sheep IgG - AF7139-SP from R&D Systems

Host Sheep
Clonality Polyclonal
Detection of Mouse Amnionless by Western Blot. Western blot shows recombinant mouse Amnionless (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Amnionless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7139) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Amnionless at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Human Amnionless Antibody - AF1860 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat

Human Amnionless Affinity Purified Polyclonal Ab, Goat IgG - AF1860-SP from R&D Systems

Host Goat
Clonality Polyclonal

Human Amnionless Biotinylated Antibody - BAF1860 from R&D Systems

Prices $390.00
Sizes 50 µg
Host Goat

Human Amnionless Antibody - MAB1860 from R&D Systems

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clone 260808

Human Amnionless MAb (Clone 260808), Mouse IgG1 - MAB1860-SP from R&D Systems

Host Mouse
Clonality Monoclonal
Clone 260808

AMN antibody - 70R-8847 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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