KDM1A
| Gene Symbol | KDM1A |
|---|---|
| Entrez Gene | 23028 |
| Alt Symbol | AOF2, BHC110, KDM1, LSD1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | lysine (K)-specific demethylase 1A |
| Other Description | BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|amine oxidase (flavin containing) domain 2|flavin-containing amine oxidase domain-containing protein 2|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1|lysine-specific histone demethylase 1A |
| Swissprots | Q5TH94 Q5TH95 O60341 Q8IXK4 A8MWP9 Q86VT7 Q96AW4 Q8TAZ3 Q8NDP6 |
| Accessions | CBY79414 CBY88366 EAW95031 EAW95032 EAW95033 O60341 AA664447 AB011173 BAA25527 AK128669 AK290328 BAF83017 AL833812 CAD38675 BC016639 AAH16639 BC025362 AAH25362 BC040194 AAH40194 BC048134 AAH48134 BG717076 BQ006926 XM_005245786 XP_005245843 XM_006710472 XP_006710535 XM_006710473 XP_006710536 XM_006710474 XP_006710537 NM_001009999 NP_001009999 NM_015013 NP_055828 |
| Function | Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag tha |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:11102443, ECO:0000269|PubMed:16079795}. |
| Tissue Specificity | Ubiquitously expressed. {ECO:0000269|PubMed:16079795}. |
LSD1 CRISPR/Cas9 KO Plasmid (h) - sc-401294 from Santa Cruz Biotechnology
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LSD1 HDR Plasmid (h) - sc-401294-HDR from Santa Cruz Biotechnology
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LSD1 Double Nickase Plasmid (h) - sc-401294-NIC from Santa Cruz Biotechnology
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LSD1 Double Nickase Plasmid (h2) - sc-401294-NIC-2 from Santa Cruz Biotechnology
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LSD1 CRISPR Activation Plasmid (h) - sc-401294-ACT from Santa Cruz Biotechnology
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LSD1 CRISPR Activation Plasmid (h2) - sc-401294-ACT-2 from Santa Cruz Biotechnology
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LSD1 Lentiviral Activation Particles (h) - sc-401294-LAC from Santa Cruz Biotechnology
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LSD1 Lentiviral Activation Particles (h2) - sc-401294-LAC-2 from Santa Cruz Biotechnology
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LSD1 siRNA (h) - sc-60970 from Santa Cruz Biotechnology
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LSD1 shRNA Plasmid (h) - sc-60970-SH from Santa Cruz Biotechnology
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LSD1 shRNA (h) Lentiviral Particles - sc-60970-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU098171 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1589 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1589 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR02388 from Sigma-Aldrich
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KDM1A siRNA (Human) - CRH7941 from Cohesion Biosciences
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LSD1 RNAi - H00023028-R01 from Novus Biologicals
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LSD1 CRISPR/Cas9 KO Plasmid (h2) - sc-401294-KO-2 from Santa Cruz Biotechnology
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LSD1 HDR Plasmid (h2) - sc-401294-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human KDM1A (NM_015013.3) - SHH324131 from Creative biogene
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shRNA set against Human AOF2(NM_001009999.2) - SHG062701 from Creative biogene
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KDM1A shRNA Plasmid (Human) - CHH7941 from Cohesion Biosciences
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KDM1A miRNA 3'UTR clone - MiUTR1H-00433 from Creative biogene
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KDM1A miRNA 3'UTR clone - MiUTR3H-10429 from Creative biogene
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