PHF19

Gene Symbol PHF19
Entrez Gene 26147
Alt Symbol MTF2L1, PCL3, TDRD19B
Species Human
Gene Type protein-coding
Description PHD finger protein 19
Other Description polycomb like 3|polycomb-like protein 3|tudor domain containing 19B
Swissprots Q8TBL6 Q5T6S4 Q32NF2 Q5T6S3 Q6N038 Q9UFS9
Accessions EAW87475 EAW87476 EAW87477 EAW87478 Q5T6S3 AA743545 AI193743 AI369987 AK122744 BAG53700 AK302996 BAH13873 AK303433 BAH13959 AL117477 CAB55950 BC022374 AAH22374 BC044224 BC108663 AAI08664 BC125076 AAI25077 BC125077 AAI25078 BM784783 BM784790 BU632411 BX640713 CAE45832 HQ447719 ADQ32204 HY013521 XM_005251906 XP_005251963 XM_006717046 XP_006717109 XM_011518509 XP_011516811 XM_011518510 XP_011516812 XM_011518511 XP_011516813 XM_011518512 XP_011516814 XM_011518513 XP_011516815 XM_011518514 XP_011516816 XM_011518515 XP_011516817 XM_011518516 XP_011516818 XM_011518517 XP_011516819 XR_929758 XR_929759 NM_001009936 NP_001009936 NM_001286840 NP_001273769 NM_001286842 NP_001273771 NM_001286843 NP_001273772 NM_015651 NP_056466 NR_104601
Function Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases NO66 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys- 36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter. {ECO:0000269|PubMed:15563832, ECO:0000269|PubMed:21143197, ECO:0000269|PubMed:23104054, ECO:0000269|PubMed:23160351}.
Subcellular Location Nucleus {ECO:0000269|PubMed:15563832}.
Tissue Specificity Isoform 1 is expressed in thymus, heart, lung and kidney. Isoform 2 is predominantly expressed in placenta, skeletal muscle and kidney, whereas isoform 1 is predominantly expressed in liver and peripheral blood leukocytes. Overexpressed in many types of cancers, including colon, skin, lung, rectal, cervical, uterus, liver cancers, in cell lines derived from different stages of melanoma and in glioma cell lines. {ECO:0000269|PubMed:15563832}.

PHF19 (D-14) - sc-243785 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PHF19 (I-13) - sc-243786 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PHF19 antibody - ab68959 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-PHF19 antibody (ab68959) at 1/500 dilutionLane 1 : transfected 293T cell lysateLane 2 : non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-PHF19 antibody - ab176586 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of PHF19 in Immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176586 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-PHF19 antibody was used at 1 µg/ml. Lane 2 represents control IgG IP.Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-PHF19 antibody - ab81164 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PHF19 antibody (ab81164) at 1 µg/ml + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PHF19 antibody - ab81166 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PHF19 antibody (ab81166) at 1 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PHF19 antibody - ab118260 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PHF19 antibody (ab118260) at 1/1000 dilution + Human fetal testis lysate

Anti-PHF19 Antibody (aa492-520, AP) - LS-C216754 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF19 Antibody (aa492-520, APC) - LS-C216756 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF19 Antibody (aa492-520, Biotin) - LS-C216757 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF19 Antibody (aa492-520, FITC) - LS-C216758 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF19 Antibody (aa492-520, PE) - LS-C216759 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF19 Antibody (aa492-520, Azide-free, HRP) - LS-C216760 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF19 Antibody (aa475-525) - LS-C290731 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF19 Antibody (aa492-520) - LS-C323988 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF19 Antibody (C-Terminus) - LS-C82605 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PHF19 Antibody (C-Terminus) - LS-C82606 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PHF19 Antibody (aa530-580) - LS-C290733 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse PHF19 by Western Blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, 293T, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PHF19 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-PHF19 Antibody (aa493-520) - LS-C158588 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PHF19 Antibody western blot of A2058 cell line lysates (35 ug/lane). The PHF19 antibody detected the PHF19 protein (arrow).

PHF19 Antibody - H00026147-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: PHF19 Antibody [H00026147-B01P] - Analysis of PHF19 expression in transfected 293T cell line by PHF19 polyclonal antibody. Lane1:PHF19 transfected lysate(22.77 KDa). Lane2:Non-transfected lysate.

PHF19 Antibody - NBP1-79352 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PHF19 Antibody [NBP1-79352] - Titration: 1 ug/ml Positive Control: HEK-293T cells.

PHF19 Antibody - NBP2-13755 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: PHF19 Antibody [NBP2-13755] - Staining of human bone marrow shows strong cytoplasmic positivity in subset of hematopoietic cells.

PHF19 Antibody - NBP1-79351 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF19 antibody produced in rabbit - HPA051458 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PHF19:<B>Cat. No. HPA051458</B>: Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subset of hematopoietic cells.

Anti-PHF19 (ab1) antibody produced in rabbit - SAB2101788 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PHF19 (ab1): <B>Cat. No.SAB2101788</B>: PHF19 in Jurkat cell lysate with PHF19 antibody at 1.0 μg/mL.
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