POLR2A
| Gene Symbol | POLR2A |
|---|---|
| Entrez Gene | 5430 |
| Alt Symbol | POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | polymerase (RNA) II (DNA directed) polypeptide A, 220kDa |
| Other Description | DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1 |
| Swissprots | A6NN93 Q6NX41 B9EH88 P24928 |
| Accessions | CAA52862 EAW90181 EAW90182 EAW90183 P24928 AK293777 BAG57192 AK304882 BAG65619 AW589665 BC067295 AAH67295 BC107140 BC137231 AAI37232 BQ353795 BQ891728 BU730469 BU739765 CV355924 DA923649 M21610 AAA60135 X63564 CAA45125 NM_000937 NP_000928 |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA h |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:9852112}. |
| Top Pathways | Huntington's disease, Herpes simplex infection, Epstein-Barr virus infection, Purine metabolism, RNA polymerase |
Pol II CRISPR/Cas9 KO Plasmid (h) - sc-400053 from Santa Cruz Biotechnology
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Pol II HDR Plasmid (h) - sc-400053-HDR from Santa Cruz Biotechnology
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Pol II Double Nickase Plasmid (h) - sc-400053-NIC from Santa Cruz Biotechnology
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Pol II Double Nickase Plasmid (h2) - sc-400053-NIC-2 from Santa Cruz Biotechnology
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Pol II CRISPR Activation Plasmid (h) - sc-400053-ACT from Santa Cruz Biotechnology
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Pol II CRISPR Activation Plasmid (h2) - sc-400053-ACT-2 from Santa Cruz Biotechnology
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Pol II Lentiviral Activation Particles (h) - sc-400053-LAC from Santa Cruz Biotechnology
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Pol II Lentiviral Activation Particles (h2) - sc-400053-LAC-2 from Santa Cruz Biotechnology
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Pol II siRNA (h) - sc-36290 from Santa Cruz Biotechnology
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Pol II shRNA Plasmid (h) - sc-36290-SH from Santa Cruz Biotechnology
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Pol II shRNA (h) Lentiviral Particles - sc-36290-V from Santa Cruz Biotechnology
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RNA Polymerase II/POLR2A RNAi - H00005430-R01 from Novus Biologicals
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MISSION® esiRNA - EHU003451 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17111 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17111 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR03495 from Sigma-Aldrich
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POLR2A siRNA (Human) - CRH3633 from Cohesion Biosciences
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shRNA set against Human POLR2A (NM_000937.4) - SHH379376 from Creative biogene
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Human POLR2A siRNA - orb264135 from Biorbyt
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POLR2A shRNA Plasmid (Human) - CHH3633 from Cohesion Biosciences
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POLR2A miRNA 3'UTR clone - MiUTR3H-02071 from Creative biogene
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