REST

Gene Symbol	REST
Entrez Gene	5978
Alt Symbol	NRSF, XBR
Species	Human
Gene Type	protein-coding
Description	RE1-silencing transcription factor
Other Description	RE1-silencing transcription factor variant E1a/E2/E3/E4c\|RE1-silencing transcription factor variant E1a/E2/E3/E5\|RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i\|RE1-silencing transcription factor variant E1a/E2/E3/N3c/E4\|RE1-silencing transcription factor variant E1a/E2/E4\|RE1-silencing transcription factor variant E1a/E2k/E2i/E3/E4j\|RE1-silencing transcription factor variant E1b/E2/E3/E5\|RE1-silencing transcription factor variant E1b/E2/E3/N3b/E4i\|RE1-silencing transcription factor variant E1b/E2/E3/N3c/E4\|RE1-silencing transcription factor variant E1b/E2c/E2j/E3/E4\|RE1-silencing transcription factor variant E1c/E2/E3/E5\|RE1-silencing transcription factor variant E1c/E2g/E3/E4\|neural-restrictive silencer factor\|neuron restrictive silencer factor\|repressor binding to the X2 box
Swissprots	Q8IWI3 Q13127 Q13134 Q12956 Q59ER1 A2RUE0 Q12957 B9EGJ0
Accessions	EAX05516 EAX05517 Q13127 AB209750 BAD92987 AF228045 AAF61710 BC017822 AAH17822 BC038985 AAH38985 BC132859 AAI32860 BC136491 AAI36492 JX896957 AFZ81807 JX896958 AFZ81808 JX896959 AFZ81809 JX896960 JX896961 JX896962 JX896963 JX896964 JX896965 JX896966 AFZ81810 JX896967 JX896968 AFZ81811 JX896969 JX896970 AFZ81812 JX896971 AFZ81813 JX896972 JX896973 JX896974 JX896975 JX896976 JX896977 JX896978 AFZ81814 JX896979 AFZ81815 JX896980 AFZ81816 JX896981 AFZ81817 JX896982 AFZ81818 JX896983 AFZ81819 JX896984 AFZ81820 JX896985 JX896986 JX896987 JX896988 JX896989 AFZ81821 JX896990 AFZ81822 JX896991 AFZ81823 JX896992 AFZ81824 JX896993 AFZ81825 KC117262 KC117263 KC117264 KC117265 KC117266 AFZ81826 U13877 AAC50114 U13879 AAC50115 U22314 AAB17211 XM_005265760 XP_005265817 XM_011534401 XP_011532703 XM_011534402 XP_011532704 NM_001193508 NP_001180437 NM_005612 NP_005603
Function	Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. {ECO:0000269\|PubMed:12399542, ECO:0000269\|PubMed:19061646, ECO:0000269\|PubMed:7697725, ECO:0000269\|PubMed:7871435, ECO:0000269\|PubMed:8568247}.
Subcellular Location	Nucleus {ECO:0000305}.
Tissue Specificity	Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. {ECO:0000269\|PubMed:8568247}.
Top Pathways	Huntington's disease, Signaling pathways regulating pluripotency of stem cells

NRSF (F-3) - sc-374611 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

NRSF (F-3): sc-374611. Western blot analysis of NRSF expression in Hep G2 nuclear extract.

NRSF (C-15) - sc-15120 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NRSF (H-290) - sc-25398 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

NRSF (H-290): sc-25398. Western blot analysis of NRSF expression in RAW 264.7 nuclear extract.

NRSF (P-18) - sc-15118 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-REST / NRSF antibody - ab21635 from Abcam

Prices	$382.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-REST / NRSF antibody (ab21635) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Mouse REST / NRSF peptide (ab17042) at 1 µg/mlLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Mouse REST / NRSF peptide (ab17042) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-REST / NRSF antibody - ab28018 from Abcam

Prices	$382.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-REST / NRSF antibody (ab28018) at 1 µg/mlLane 1 : HEK293 LysateLane 2 : Hela LysateLysates/proteins at 20 µg per lane.

Anti-REST / NRSF antibody - ab202962 from Abcam

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of formalin-fixed paraffin-embedded Mouse brain tissue labeling REST / NRSF with ab202962 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Anti-REST / NRSF antibody - ab52849 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-REST / NRSF antibody (ab52849) at 1/1000 dilutionLane 1 : Total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigenLane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (Tagged fusion protein)Lysates/proteins at 20 µg per lane.SecondaryRabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution

Anti-REST / NRSF antibody - ab70300 from Abcam

Prices	$378.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling REST / NRSF with ab70300 at 1/1000 (1µg/ml). Detection: DAB.

Anti-REST / NRSF antibody [EPR2436Y] - ab75785 from Abcam

Prices	$381.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR2436Y

All lanes : Anti-REST / NRSF antibody [EPR2436Y] (ab75785) at 1/2000 dilutionLane 1 : Raji cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-NRSF / REST Antibody (aa801-1097) - LS-C17676 from LifeSpan Bioscience

Prices	$515.00
Sizes	200 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (aa800-850) - LS-C90908 from LifeSpan Bioscience

Prices	$545.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (C-Terminus) - LS-C90909 from LifeSpan Bioscience

Prices	$635.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (aa542-570, AP) - LS-C223854 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (aa542-570, APC) - LS-C223855 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (aa542-570, Biotin) - LS-C223856 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (aa542-570, FITC) - LS-C223857 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (aa542-570, Azide-free, HRP) - LS-C223858 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (aa542-570, PE) - LS-C223859 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (aa20-69) - LS-C145441 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

REST antibody LS-C145441 Western blot of Mouse Heart lysate. Antibody concentration 1 ug/ml.

Anti-NRSF / REST Antibody (aa541-570) - LS-C324613 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NRSF / REST Antibody (aa972-1021) - LS-C109670 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

REST antibody LS-C109670 Western blot of MCF7 cell lysate.

Anti-NRSF / REST Antibody (aa873-877) - LS-C205117 from LifeSpan Bioscience

Prices	$265.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Western blot of extract from HeLa cells using REST antibody

Anti-NRSF / REST Antibody (N-Terminus, clone EPR2436Y) - LS-C105377 from LifeSpan Bioscience

Prices	$480.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR2436Y

Western blot analysis on (A) Raji and (B) HeLa cell lysates using anti-REST antibody (LS-C105377), dilution 1:2000.

Anti-NRSF / REST Antibody (aa542-570) - LS-C162546 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

REST Antibody western blot of Jurkat cell line lysates (35 ug/lane). The REST antibody detected the REST protein (arrow).

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