UFL1

Gene Symbol UFL1
Entrez Gene 23376
Alt Symbol KIAA0776, Maxer, NLBP, RCAD
Species Human
Gene Type protein-coding
Description UFM1-specific ligase 1
Other Description E3 UFM1-protein ligase 1|LZAP-binding protein|Regulator of CDK5RAP3 and DDRGK1|novel LZAP-binding protein|regulator of C53/LZAP and DDRGK1
Swissprots C0H5X5 B4DJ57 A0PJ53 Q8N765 O94874 Q9NTQ0
Accessions EAW48506 EAW48507 EAW48508 O94874 AB018319 BAA34496 AJ420579 AK295934 BAG58719 AK307651 AK316453 BAH14824 BC015377 AAH15377 BC028608 AAH28608 BC036379 AAH36379 BP337693 NM_015323 NP_056138
Function E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, a post-translational modification on lysine residues of proteins that may play a crucial role in a number of cellular processes. Mediates DDRGK1 ufmylation and may regulate the proteasomal degradation of DDRGK1 and CDK5RAP3 thereby modulating NF-kappa-B signaling (PubMed:20018847, PubMed:20164180, PubMed:20228063, PubMed:25219498). May also through TRIP4 ufmylation play a role in nuclear receptors-mediated transcription (PubMed:25219498). {ECO:0000269|PubMed:20018847, ECO:0000269|PubMed:20164180, ECO:0000269|PubMed:20228063, ECO:0000269|PubMed:25219498}.
Subcellular Location Endoplasmic reticulum {ECO:0000269|PubMed:20018847, ECO:0000269|PubMed:20164180, ECO:0000269|PubMed:20228063}. Cytoplasm, cytosol {ECO:0000269|PubMed:20228063}.
Tissue Specificity Ubiquitously expressed, with a high expression in liver (at protein level). Low expression in several invasive hepatocellular carcinomas, such Hep-G2, Hep 3B2.1-7, HLE and PLC. {ECO:0000269|PubMed:20018847}.

KIAA0776 (N-13) - sc-168341 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-K0776 antibody - ab103047 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-K0776 antibody (ab103047) at 1/100 dilution + MCF7 cell lysate at 35 µg

Anti-K0776 antibody - ab123500 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123500 for IP at 6 µg/mg lysate.Subsequent Western blot detection used an antibody which recognized a downstream epitope of K0776 at 0.4 µg/ml.Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-KIAA0776 / NLBP Antibody (aa426-455, APC) - LS-C260766 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0776 / NLBP Antibody (aa426-455, AP) - LS-C260765 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0776 / NLBP Antibody (aa426-455, FITC) - LS-C260768 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0776 / NLBP Antibody (aa426-455, Biotin) - LS-C260767 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0776 / NLBP Antibody (aa426-455, Azide-free, HRP) - LS-C260769 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0776 / NLBP Antibody (aa426-455, PE) - LS-C260770 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0776 / NLBP Antibody (aa175-225) - LS-C290130 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0776 / NLBP Antibody (aa744-794) - LS-C290131 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0776 / NLBP Antibody (aa426-455) - LS-C165385 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of K0776 Antibody in MCF-7 cell line lysates (35 ug/lane). K0776 (arrow) was detected using the purified antibody.

Anti-KIAA0776 / NLBP Antibody (aa433-463) - LS-C322345 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA0776 / NLBP Antibody (aa503-552) - LS-C111618 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
KIAA0776 antibody LS-C111618 Western blot of Fetal Kidney lysate.

UFL1 Antibody - NBP1-90691 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UFL1 Antibody [NBP1-90691] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

UFL1 Antibody - NBP1-79039 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UFL1 Antibody [NBP1-79039] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ULF1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ULF1 was also immunoprecipitated by rabbit anti-ULF1 antibody NBP1-79038 which recognizes an upstream epitope.

UFL1 Antibody - NBP1-90692 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UFL1 Antibody [NBP1-90692] - Analysis in human cell line Daudi.

UFL1 Antibody - NBP1-55438 from Novus Biologicals

Prices $369.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: UFL1 Antibody [NBP1-55438] - Human kidney lysate, concentration 0.2-1 ug/ml.

Anti-KIAA0776 (741-755) antibody produced in rabbit - SAB1101574 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-KIAA0776 (741-755) (RB): <B>Cat. No. SAB1101574</B>: Immunofluorescence of HUVEC cells using Anti-KIAA0776 (741-755) (RB) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-UFL1 antibody produced in rabbit - HPA030560 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-KIAA0776: <B>Cat. No. HPA030560</B>: Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Anti-UFL1 antibody produced in rabbit - HPA030558 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-KIAA0776: <B>Cat. No. HPA030558</B>: Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in subsets of tubules.

Anti-UFL1 antibody produced in rabbit - HPA030559 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-KIAA0776: <B>Cat. No. HPA030559</B>: Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

KIAA0776 antibody - middle region (ARP55228_P050) - ARP55228_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

K0776-Antibody-Center - AP9677c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of K0776 Antibody (Center) (Cat. #AP9677c) in MCF-7 cell line lysates (35ug/lane). K0776 (arrow) was detected using the purified Pab.

KIAA0776 Antibody - middle region (OAAB06273) - OAAB06273 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
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