IMPACT

Gene Symbol IMPACT
Entrez Gene 55364
Alt Symbol RWDD5
Species Human
Gene Type protein-coding
Description impact RWD domain protein
Other Description Impact homolog|RWD domain containing 5|imprinted and ancient gene protein homolog|protein IMPACT
Swissprots Q9H2X4 A8MXG0 Q49AM0 Q9P2X3
Accessions AAG23917 AAS93618 CAV24109 CBF65942 CBU90814 EAX01184 EAX01185 EAX01186 EAX01187 EAX01188 Q9P2X3 AB026264 BAA95160 AF208694 AAG35736 AK292533 BAF85222 AK312727 BC034016 AAH34016 BC036074 AAH36074 BP384088 DQ893141 ABM84067 EU176771 ABW03572 NM_018439 NP_060909
Function Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis (By similarity). May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis (By similarity). Through its action on GCN2, may also facilitate neuritogenesis (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000250}. Note=May be associated with polysomes. {ECO:0000250}.
Tissue Specificity Widely expressed. Expressed at high level in brain. {ECO:0000269|PubMed:11116084, ECO:0000269|PubMed:11244491}.

IMPACT (V-20) - sc-84853 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

IMPACT (K-19) - sc-84851 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-IMPACT antibody - ab72444 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-IMPACT antibody (ab72444) + Rat brainSecondaryHRP-conjugated goat anti-mouse IgG (H&L)

Anti-IMPACT antibody - ab95175 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-IMPACT antibody - N-terminal - ab173167 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-IMPACT antibody - N-terminal (ab173167) at 1/100 dilution + HeLa cell lysate at 35 µg

Anti-IMPACT Antibody (aa1-30, APC) - LS-C260215 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IMPACT Antibody (aa1-30, Biotin) - LS-C260216 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IMPACT Antibody (aa1-30, FITC) - LS-C260217 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IMPACT Antibody (aa1-30, Azide-free, HRP) - LS-C260218 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IMPACT Antibody (aa1-30, PE) - LS-C260219 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IMPACT Antibody (aa270-320) - LS-C289381 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-IMPACT Antibody - LS-C308486 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-IMPACT Antibody (aa1-30) - LS-C322236 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-IMPACT Antibody (aa148-301) - LS-C311894 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-IMPACT Antibody (aa1-30) - LS-C159922 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
IMPACT Antibody western blot of HeLa cell line lysates (35 ug/lane). The IMPACT antibody detected the IMPACT protein (arrow).

Anti-IMPACT Antibody (aa1-30, AP) - LS-C260213 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

IMPACT Antibody - NBP1-86221 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: IMPACT Antibody [NBP1-86221] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

IMPACT Antibody - H00055364-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: IMPACT Antibody [H00055364-B01P] - Analysis of IMPACT expression in rat brain.

IMPACT Antibody - NBP1-42678 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: IMPACT Antibody [NBP1-42678] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-IMPACT antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. IMPACT was also immunoprecipitated by another rabbit anti-IMPACT antibody which recognizes an upstream epitope.

IMPACT Antibody - NBP1-86234 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-IMPACT antibody produced in rabbit - HPA041968 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-IMPACT: <B>Cat. No. HPA041968</B>: Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-IMPACT antibody produced in rabbit - HPA041045 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-IMPACT: <B>Cat. No. HPA041045</B>: Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.

Anti-IMPACT antibody produced in mouse - SAB1400615 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Polyclonal Anti-IMPACT, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1400615</B>: Antibody concentration: 1.0 ~ 5.0 μg/mL. Specific band of ~36.5 kDa using rat brain lysate.

IMPACT-Antibody-N-term - AP17553a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
IMPACT Antibody (N-term) (Cat. #AP17553a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IMPACT antibody detected the IMPACT protein (arrow).

IMPACT antibody [N2C3] - GTX119445 from GeneTex

Host Rabbit
Clonality Polyclonal
Search more