NSMF

Gene Symbol	NSMF
Entrez Gene	26012
Alt Symbol	HH9, NELF
Species	Human
Gene Type	protein-coding
Description	NMDA receptor synaptonuclear signaling and neuronal migration factor
Other Description	nasal embryonic LHRH factor\|nasal embryonic luteinizing hormone-releasing hormone factor
Swissprots	Q9NPS3 Q96SY1 Q9NPM4 Q8N2M2 Q6X4V9 Q2TB96 Q9NPP3 Q6X4V8 Q6X4W1 Q6X4V7
Accessions	CAF85849 EAW88390 EAW88391 EAW88392 EAW88393 EAW88394 Q6X4W1 AJ420418 AK027474 BAB55139 AK074602 BAC11086 AK292030 BAF84719 AK294320 BAG57594 AK303703 BAG64688 AK303976 BAH14089 AK316046 BAH14417 AL117660 CAB56031 AL389944 CAB97524 AL389945 CAB97542 AL389946 CAB97525 AW072125 AY255128 AAP83576 AY255129 AAP83577 AY255130 AAP83578 AY255131 AAP83579 AY255132 AAP83580 BC004318 AAH04318 BC016862 AAH16862 BC072412 AAH72412 BC110498 AAI10499 BM023184 CN306732 XM_005266061 XP_005266118 XM_005266062 XP_005266119 XM_011518496 XP_011516798 XM_011518497 XP_011516799 NM_001130969 NP_001124441 NM_001130970 NP_001124442 NM_001130971 NP_001124443 NM_001178064 NP_001171535 NM_015537 NP_056352
Function	Couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. Part of the cAMP response element-binding protein (CREB) shut-off signaling pathway. Stimulates outgrowth of olfactory axons and migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neuronal cells. {ECO:0000269\|PubMed:20025934}.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:20025934}. Nucleus envelope {ECO:0000250}. Nucleus membrane {ECO:0000250}. Nucleus matrix {ECO:0000250}. Cytoplasm {ECO:0000269\|PubMed:20025934}. Cytoplasm, cell cortex {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cell membrane {ECO:0000269\|PubMed:20025934}; Peripheral membrane protein {ECO:0000269\|PubMed:20025934}. Cell projection, dendrite {ECO:0000250}. Cell junction, synapse {ECO:0000250}. Cell junction, synapse, synaptosome {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Membrane {ECO:0000250}. Note=Found on the outside of the luteinizing-hormone-releasing hormone (LHRH) cell membrane and axons projecting from the olfactory pit and epithelium. Associates with transcriptionally active chromatin regions. Detected at the nuclear membranes of CA1 neurons. Cortical cytoskeleton. Localized in proximal apical dendrites. Colocalizes with CABP1 in dendrites and dendritic spines. Myristoylation is a
Tissue Specificity	Highly expressed in adult and fetal brain. Weakly expressed in heart, liver, spleen, testis, small intestine, skeletal muscle, peripheral white blood cells and kidney. {ECO:0000269\|PubMed:15362570, ECO:0000269\|PubMed:20025934}.

NELF (N-17) - sc-160578 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NELF (N-17): sc-160578. Western blot analysis of NELF expression in COLO 205 (A) and K-562 (B) whole cell lysates.

NELF (E-14) - sc-160577 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NELF (H-24) - sc-133809 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

Anti-NELF antibody - ab189615 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-NELF antibody (ab189615) at 1/500 dilutionLane 1 : Mouse thymus total cell extractLane 2 : HeLa total cell extractLane 3 : Jurkat total cell extract

Anti-NELF antibody - ab130587 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NELF antibody (ab130587) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg

Anti-NELF antibody - ab58752 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NELF antibody (ab58752) at 5 µg/ml + HepG2 Cell LysateSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilutionObserved band size : 29 kDa (why is the actual band size different from the predicted?)

Anti-NELF antibody [EPR8976(2)] - ab183022 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR8976(2)

Flow cytometry analysis of PC3 cells labeling NELF using ab183022 at 1/300 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.

Anti-NSMF / NELF Antibody (aa500-530, AP) - LS-C215951 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSMF / NELF Antibody (aa500-530, APC) - LS-C215952 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSMF / NELF Antibody (aa500-530, Biotin) - LS-C215954 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSMF / NELF Antibody (aa500-530, FITC) - LS-C215955 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSMF / NELF Antibody (aa500-530, Azide-free, HRP) - LS-C215956 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSMF / NELF Antibody (aa500-530, PE) - LS-C215957 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSMF / NELF Antibody (aa500-530) - LS-C329882 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSMF / NELF Antibody (aa283-332) - LS-C31893 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NSMF / NELF Antibody (N-Terminus) - LS-C110691 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

NELF antibody LS-C110691 Western blot of Fetal Liver lysate.

Anti-NSMF / NELF Antibody (N-Terminus) - LS-C345835 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of NSMF Antibody with human 293T Whole Cell lysate.

Anti-NSMF / NELF Antibody (aa501-530) - LS-C158376 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

NELF Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The NELF antibody detected the NELF protein (arrow).

Anti-NSMF / NELF Antibody (Internal) - LS-C201043 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of NELF in rat brain tissue with NELF antibody at 2.5 ug/ml.

NELF Antibody - NBP1-54813 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: NELF Antibody [NBP1-54813] - Titration: 0.2-1 ug/ml, Positive Control: Human Liver.

NELF Antibody - NBP1-54835 from Novus Biologicals

Prices	$139.00, $299.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: NELF Antibody [NBP1-54835] - Titration: 5.0ug/ml Positive Control: HepG2 cell lysate.

NELF Antibody - NBP1-92175 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: NELF Antibody [NBP1-92175] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

NELF Polyclonal Antibody - bs-11893R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

NELF Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-11893R-A350 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

NELF Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-11893R-A488 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

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