URM1

Gene Symbol URM1
Entrez Gene 81605
Alt Symbol C9orf74
Species Human
Gene Type protein-coding
Description ubiquitin related modifier 1
Other Description ubiquitin related modifier 1 homolog|ubiquitin-related modifier 1|ubiquitin-related modifier 1 homolog
Swissprots B3KMH3 B4DV08 Q9BTM9 Q5T4B4
Accessions EAW87782 EAW87783 EAW87784 EAW87785 EAW87786 Q9BTM9 AI872337 AK001880 BAG50985 AK097029 AK300877 BAG62520 BC003581 AAH03581 BC011620 BI823604 D83200 DA691216 HG502453 NM_001135947 NP_001129419 NM_001265582 NP_001252511 NM_030914 NP_112176 NR_049743
Function Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates. {ECO:0000255|HAMAP- Rule:MF_03048, ECO:0000269|PubMed:19017811, ECO:0000269|PubMed:21209336}.
Subcellular Location Cytoplasm.
Top Pathways Sulfur relay system

Urm1 (A-7) - sc-374485 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Urm1 (A-7): sc-374485. Western blot analysis of Urm1 expression in T-47D (A) and Hep G2 (B) whole cell lysates.

Urm1 (H-78) - sc-292073 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Urm1 (K-15) - sc-163508 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Urm1 (T-14) - sc-163509 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Urm1 (N-24) - sc-130288 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Urm1 (N-24): sc-130288. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.

Anti-Urm1 antibody [EPR4809] - ab110699 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4809
All lanes : Anti-Urm1 antibody [EPR4809] (ab110699) at 1/1000 dilutionLane 1 : Human fetal liver lysate at 10 µgLane 2 : A549 lysate at 10 µgLane 3 : Human fetal lung lysate at 10 µgLane 4 : HepG2 lysate at 10 µgLane 5 : DDDDK-tagged Urm1 recombinant protein at 0.01 µg

Anti-URM1 Antibody - LS-C160459 from LifeSpan Bioscience

Prices $250.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with URM1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Anti-URM1 Antibody - LS-C59546 from LifeSpan Bioscience

Prices $395.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody - LS-C93236 from LifeSpan Bioscience

Prices $575.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody (N-Terminus, AP) - LS-C226613 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody (N-Terminus, APC) - LS-C226614 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody (N-Terminus, Biotin) - LS-C226615 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody (N-Terminus, FITC) - LS-C226616 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody (N-Terminus, PE) - LS-C226618 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody (N-Terminus, Azide-free, HRP) - LS-C226617 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody (N-Terminus) - LS-C329014 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody (Internal, clone EPR4809) - LS-C138350 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4809
Western blot analysis on (A) fetal liver, (B) A549, (C) fetal lung, (D) HepG2, and (E) Flag-tagged URM1 recombinant protein (10 ng) lysates using anti-URM1 antibody.

Anti-URM1 Antibody - LS-C343810 from LifeSpan Bioscience

Prices $235.00
Sizes 25 µl
Host Rabbit
Clonality Polyclonal

Anti-URM1 Antibody - LS-C160458 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
URM1 antibody western blot of HepG2 cell line lysates (35 ug/lane). The URM1 antibody detected the URM1 protein (arrow).

Anti-URM1 Antibody (aa8-40) - LS-C97819 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with URM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

URM1 Antibody (EPR4809) - NBP1-95124 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4809
Western Blot: URM1 Antibody (EPR4809) [NBP1-95124] (A) fetal liver, (B) A549, © fetal lung, (D) HepG2, and (E) Flag-tagged URM1 recombinant protein (10 ng) lysates.

URM1 Antibody - NBP2-39047 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: URM1 Antibody [NBP2-39047] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

URM1 Antibody - NB600-469 from Novus Biologicals

Prices $399.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal
Western Blot: URM1 Antibody [NB600-469] - Anti- Urm1 antibody generated by immunization with recombinant yeast Urm1 was tested by western blot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Urm1 using a 1:1,000 dilution of IgG fraction of Rabbit-anti- Urm1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4 C.

Anti-URM1 (N-term) antibody produced in rabbit - SAB1300218 from Sigma-Aldrich

Prices $291.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-URM1 (N-term) Antibody: <B>Cat. No. SAB1300218</B>: URM1 in human breast carcinoma tissue with Anti-URM1 (N-term) Antibody at 40 μg/mL.

ANTI-URM1 antibody produced in mouse - SAB1306731 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-URM1 Monoclonal : <B>Cat. No. SAB1306731</B>: URM1 Antibody western blot analysis in HepG2 cell line lysates (35 μg/lane).
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