Gene Symbol | URM1 |
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Entrez Gene | 81605 |
Alt Symbol | C9orf74 |
Species | Human |
Gene Type | protein-coding |
Description | ubiquitin related modifier 1 |
Other Description | ubiquitin related modifier 1 homolog|ubiquitin-related modifier 1|ubiquitin-related modifier 1 homolog |
Swissprots | B3KMH3 B4DV08 Q9BTM9 Q5T4B4 |
Accessions | EAW87782 EAW87783 EAW87784 EAW87785 EAW87786 Q9BTM9 AI872337 AK001880 BAG50985 AK097029 AK300877 BAG62520 BC003581 AAH03581 BC011620 BI823604 D83200 DA691216 HG502453 NM_001135947 NP_001129419 NM_001265582 NP_001252511 NM_030914 NP_112176 NR_049743 |
Function | Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates. {ECO:0000255|HAMAP- Rule:MF_03048, ECO:0000269|PubMed:19017811, ECO:0000269|PubMed:21209336}. |
Subcellular Location | Cytoplasm. |
Top Pathways | Sulfur relay system |
Urm1 CRISPR/Cas9 KO Plasmid (h) - sc-405624 from Santa Cruz Biotechnology
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Urm1 HDR Plasmid (h) - sc-405624-HDR from Santa Cruz Biotechnology
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Urm1 Double Nickase Plasmid (h) - sc-405624-NIC from Santa Cruz Biotechnology
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Urm1 Double Nickase Plasmid (h2) - sc-405624-NIC-2 from Santa Cruz Biotechnology
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Urm1 CRISPR Activation Plasmid (h) - sc-405624-ACT from Santa Cruz Biotechnology
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Urm1 CRISPR Activation Plasmid (h2) - sc-405624-ACT-2 from Santa Cruz Biotechnology
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Urm1 Lentiviral Activation Particles (h) - sc-405624-LAC from Santa Cruz Biotechnology
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Urm1 Lentiviral Activation Particles (h2) - sc-405624-LAC-2 from Santa Cruz Biotechnology
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Urm1 siRNA (h) - sc-92844 from Santa Cruz Biotechnology
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Urm1 shRNA Plasmid (h) - sc-92844-SH from Santa Cruz Biotechnology
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Urm1 shRNA (h) Lentiviral Particles - sc-92844-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU009021 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22542 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22542 from Sigma-Aldrich
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URM1 siRNA (Human) - CRJ3015 from Cohesion Biosciences
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URM1 - MBS8226336 from MyBioSource
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shRNA set against Human URM1 (NM_030914.3) - SHH441032 from Creative biogene
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Human URM1 siRNA - orb273516 from Biorbyt
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URM1 shRNA Plasmid (Human) - CHJ3015 from Cohesion Biosciences
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URM1 miRNA 3'UTR clone - MiUTR3H-12550 from Creative biogene
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URM1 miRNA 3'UTR clone - MiUTR3H-12551 from Creative biogene
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