UBE2K

Gene Symbol UBE2K
Entrez Gene 3093
Alt Symbol E2-25K, HIP2, HYPG, LIG, UBC1
Species Human
Gene Type protein-coding
Description ubiquitin-conjugating enzyme E2K
Other Description E2(25K)|HIP-2|huntingtin interacting protein 2|huntingtin-interacting protein 2|ubiquitin carrier protein|ubiquitin-conjugating enzyme E2 K|ubiquitin-conjugating enzyme E2(25K)|ubiquitin-conjugating enzyme E2-25 KDA|ubiquitin-conjugating enzyme E2-25K|ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)|ubiquitin-protein ligase
Swissprots B2RDF8 Q9Y2D3 P61086 P27924 A8K5Y9 Q9CVV9 A6NJC1 C9JGP1 O54806 Q16721
Accessions EAW92946 EAW92947 EAW92948 EAW92949 EAW92950 P61086 AB022435 BAA78555 AB022436 BAA78556 AK093533 BAG52736 AK291454 BAF84143 AK295846 BAG58654 AK307130 AK315524 BAG37905 AL117568 BC022804 AAH22804 BC050600 AAH50600 BU622273 BX641089 CX786829 DA526283 EU446896 ABZ92425 U58522 AAC50633 NM_001111112 NP_001104582 NM_001111113 NP_001104583 NM_005339 NP_005330
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In c
Subcellular Location Cytoplasm {ECO:0000250}.
Tissue Specificity Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. {ECO:0000269|PubMed:10634809, ECO:0000269|PubMed:10675012, ECO:0000269|PubMed:8702625}.
Top Pathways Ubiquitin mediated proteolysis

HIP2 (D27C4) Rabbit mAb - 8226 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D27C4
Western blot analysis of extracts from various cell lines using HIP2 (D27C4) Rabbit mAb.

E2-25K/Hip2 Antibody - 3847 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using E2-25K/Hip2 Antibody.

HIP2 (H-6) - sc-390339 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HIP2 (H-6): sc-390339. Western blot analysis of HIP2 expression in non-transfected 293T: sc-117752 (A), human HIP2 transfected 293T: sc-116355 (B) and SP2/0 (C) whole cell lysates and human spleen tissue extract (D).

HIP2 (A-15) - sc-50733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HIP2 (A-15): sc-50733. Western blot analysis of HIP2 expression in Jurkat whole cell lysate.

HIP2 (Q-16) - sc-50736 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HIP2 (Q-16): sc-50736. Western blot analysis of HIP2 expression in non-transfected: sc-117752 (A) and human HIP2 transfected: sc-116355 (B) 293T whole cell lysates.

HIP2 (H-100) - sc-366111 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HIP2 (H-100): sc-366111. Western blot analysis of HIP2 expression in Jurkat (A) and SP2/0 (B) whole cell lysates and human spleen tissue extract (C).

HIP2 (757C3a) - sc-81286 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HIP2 (757C3a): sc-81286 Western Blot analysis of human recombinant HIP2 fusion protein.

HIP2 (C-5) - sc-390138 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HIP2 (C-5): sc-390138. Western blot analysis of HIP2 expression in Jurkat (A) and SP2/0 (B) whole cell lysates and human spleen tissue extract (C).

Anti-HIP2 antibody - ab21728 from Abcam

Prices $359.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
HIP2 staining (0.5 µg/ml) of Jurkat lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-HIP2 antibody [757C3a] - ab50837 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 757C3a
Anti-HIP2 antibody [757C3a] (ab50837)

Anti-HIP2 antibody [EP1144Y] - ab68162 from Abcam

Prices $304.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1144Y
All lanes : Anti-HIP2 antibody [EP1144Y] (ab68162) at 1/500 dilutionLane 1 : Daudi cell lysateLane 2 : Jurkat cell lysateLane 3 : HepG2 cell lysateLane 4 : human brain cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-HIP2 antibody [EP1145Y] - ab52930 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1145Y
Anti-HIP2 antibody [EP1145Y] (ab52930) at 1/100000 dilution + Daudi cell lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled at 1/2000 dilution

Anti-UBE2K / LIG Antibody (aa144-157) IHC-plus™ - LS-B4927 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-UBE2K / HIP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.

Anti-UBE2K / LIG Antibody (Arg11) - LS-C40067 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2K / LIG Antibody (clone 757C3a) - LS-C76656 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 757C3a

Anti-UBE2K / LIG Antibody (N-Terminus) - LS-C31921 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Jurkat Cell Lysate

Anti-UBE2K / LIG Antibody (clone 757C3a) - LS-C184407 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 757C3a

Anti-UBE2K / LIG Antibody (aa2-30, AP) - LS-C272897 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2K / LIG Antibody (aa2-30, APC) - LS-C272898 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2K / LIG Antibody (aa2-30, Biotin) - LS-C272899 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2K / LIG Antibody (aa36-85) - LS-C102735 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
UBE2K / HIP2 antibody LS-C102735 Western blot of HepG2 cell lysate.

Anti-UBE2K / LIG Antibody (aa2-30, FITC) - LS-C272900 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2K / LIG Antibody (aa2-30, Azide-free, HRP) - LS-C272901 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2K / LIG Antibody (aa2-30, PE) - LS-C272902 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2K / LIG Antibody - LS-C285693 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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