Gene Symbol | STUB1 |
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Entrez Gene | 10273 |
Alt Symbol | CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7, UBOX1 |
Species | Human |
Gene Type | protein-coding |
Description | STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase |
Other Description | CLL-associated antigen KW-8|E3 ubiquitin-protein ligase CHIP|antigen NY-CO-7|carboxy terminus of Hsp70-interacting protein|heat shock protein A binding protein 2 (c-terminal)|serologically defined colon cancer antigen 7 |
Swissprots | O60526 Q969U2 A2IDB9 Q9HBT1 Q9UNE7 |
Accessions | AAK61242 CAV30716 EAW85756 EAW85757 EAW85758 Q9UNE7 AF039689 AAC18038 AF129085 AAD33400 AF217968 AAG17211 AF432221 AAL99927 BC007545 AAH07545 BC017178 AAH17178 BC022788 AAH22788 BC063617 AAH63617 CB145452 DQ893185 ABM84111 DQ896502 ABM87501 NM_001293197 NP_001280126 NM_005861 NP_005852 |
Function | E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Negatively regu |
Subcellular Location | Cytoplasm {ECO:0000269|PubMed:10330192, ECO:0000269|PubMed:23973223}. Nucleus {ECO:0000269|PubMed:23973223}. Note=Translocates to the nucleus in response to inflammatory signals in regulatory T-cells (Treg). {ECO:0000269|PubMed:23973223}. |
Tissue Specificity | Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. {ECO:0000269|PubMed:10330192, ECO:0000269|PubMed:11435423}. |
Top Pathways | Ubiquitin mediated proteolysis, Protein processing in endoplasmic reticulum |
CHIP CRISPR/Cas9 KO Plasmid (h) - sc-400815 from Santa Cruz Biotechnology
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CHIP HDR Plasmid (h) - sc-400815-HDR from Santa Cruz Biotechnology
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CHIP Double Nickase Plasmid (h) - sc-400815-NIC from Santa Cruz Biotechnology
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CHIP Double Nickase Plasmid (h2) - sc-400815-NIC-2 from Santa Cruz Biotechnology
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CHIP CRISPR Activation Plasmid (h) - sc-400815-ACT from Santa Cruz Biotechnology
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CHIP CRISPR Activation Plasmid (h2) - sc-400815-ACT-2 from Santa Cruz Biotechnology
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CHIP Lentiviral Activation Particles (h) - sc-400815-LAC from Santa Cruz Biotechnology
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CHIP Lentiviral Activation Particles (h2) - sc-400815-LAC-2 from Santa Cruz Biotechnology
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CHIP siRNA (h) - sc-43555 from Santa Cruz Biotechnology
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CHIP shRNA Plasmid (h) - sc-43555-SH from Santa Cruz Biotechnology
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CHIP shRNA (h) Lentiviral Particles - sc-43555-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU106761 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN20774 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND20774 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR12041 from Sigma-Aldrich
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STUB1 siRNA (Human) - CRH6955 from Cohesion Biosciences
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STUB1 - MBS8218676 from MyBioSource
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CHIP CRISPR/Cas9 KO Plasmid (h2) - sc-400815-KO-2 from Santa Cruz Biotechnology
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CHIP HDR Plasmid (h2) - sc-400815-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human STUB1(NM_005861.2) - SHL033960 from Creative biogene
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Human STUB1 siRNA - orb267457 from Biorbyt
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STUB1 shRNA Plasmid (Human) - CHH6955 from Cohesion Biosciences
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STUB1 miRNA 3'UTR clone - MiUTR1H-10034 from Creative biogene
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