| Gene Symbol | VPS4A |
|---|---|
| Entrez Gene | 27183 |
| Alt Symbol | SKD1, SKD1A, SKD2, VPS4, VPS4-1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | vacuolar protein sorting 4 homolog A (S. cerevisiae) |
| Other Description | SKD1-homolog|hVPS4|vacuolar protein sorting factor 4A|vacuolar protein sorting-associated protein 4A|vacuolar sorting protein 4 |
| Swissprots | Q9UN37 B2RCB7 Q8TF07 Q9Y582 Q9UI03 |
| Accessions | AAG01470 EAW83261 EAW83262 EAW83263 EAW83264 Q9UN37 AF112215 AAF17203 AF132747 AAL75948 AF155740 AAD42971 AF159063 AAD49227 AF255952 AAK52408 AK000200 BAA91005 AK025850 AK315026 BAG37514 AL133634 CAB63758 BC013021 BC035121 AAH35121 BC047932 AAH47932 EU831747 ACE86581 NM_013245 NP_037377 |
| Function | Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphory |
| Subcellular Location | Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Midbody. Note=Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody (PubMed:24814515). Localized in two distinct rings on either side of the Fleming body. {ECO:0000269|PubMed:24814515}. |
| Tissue Specificity | Ubiquitously expressed. {ECO:0000269|PubMed:10637304, ECO:0000269|PubMed:11563910}. |
| Top Pathways | Endocytosis |
VPS4A CRISPR/Cas9 KO Plasmid (h) - sc-402005 from Santa Cruz Biotechnology
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VPS4A HDR Plasmid (h) - sc-402005-HDR from Santa Cruz Biotechnology
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VPS4A Double Nickase Plasmid (h) - sc-402005-NIC from Santa Cruz Biotechnology
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VPS4A Double Nickase Plasmid (h2) - sc-402005-NIC-2 from Santa Cruz Biotechnology
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VPS4A CRISPR Activation Plasmid (h) - sc-402005-ACT from Santa Cruz Biotechnology
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VPS4A CRISPR Activation Plasmid (h2) - sc-402005-ACT-2 from Santa Cruz Biotechnology
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VPS4A Lentiviral Activation Particles (h) - sc-402005-LAC from Santa Cruz Biotechnology
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VPS4A Lentiviral Activation Particles (h2) - sc-402005-LAC-2 from Santa Cruz Biotechnology
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VPS4A siRNA (h) - sc-106731 from Santa Cruz Biotechnology
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VPS4A shRNA Plasmid (h) - sc-106731-SH from Santa Cruz Biotechnology
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VPS4A shRNA (h) Lentiviral Particles - sc-106731-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU033401 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22728 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22728 from Sigma-Aldrich
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VPS4A siRNA (Human) - CRH9014 from Cohesion Biosciences
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VPS4A RNAi - H00027183-R01 from Novus Biologicals
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VPS4A - MBS8217698 from MyBioSource
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shRNA set against Human VPS4A(NM_013245.2) - SHL128154 from Creative biogene
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VPS4A shRNA Plasmid (Human) - CHH9014 from Cohesion Biosciences
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VPS4A miRNA 3'UTR clone - MiUTR1H-11160 from Creative biogene
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