SRSF2

Gene Symbol SRSF2
Entrez Gene 6427
Alt Symbol PR264, SC-35, SC35, SFRS2, SFRS2A, SRp30b
Species Human
Gene Type protein-coding
Description serine/arginine-rich splicing factor 2
Other Description SR splicing factor 2|splicing component, 35 kDa|splicing factor SC35|splicing factor, arginine/serine-rich 2
Swissprots B4DN89 H0YG49 B3KWD5 Q01130
Accessions AAA60162 CAA53383 CAH25911 EAW89439 EAW89440 EAW89441 EAW89442 EAW89443 EAW89444 EAW89445 EAW89446 EAW89447 EAW89448 Q01130 AK092489 BAC03903 AK093626 BAC04206 AK097069 BAG53419 AK098209 BAG53593 AK123712 BAG53944 AK124792 BAG54097 AK126432 BAG54327 AK223252 BAD96972 AK297813 BAG60151 BC000339 AAH00339 BC001303 AAH01303 BC063570 BC066958 AAH66958 BC070086 AAH70086 BT007250 AAP35914 DA513281 DC361254 DQ891210 ABM82136 DQ894393 ABM85319 EB388696 M90104 AAA60306 X62447 CAA44307 XR_429913 XR_429914 XR_934515 XR_934516 NM_001195427 NP_001182356 NM_003016 NP_003007 NR_036608
Function Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. {ECO:0000269|PubMed:19592491, ECO:0000269|PubMed:21157427}.
Subcellular Location Nucleus {ECO:0000269|PubMed:21157427}. Nucleus, nucleoplasm {ECO:0000269|PubMed:21157427}. Nucleus speckle {ECO:0000269|PubMed:21157427}. Note=Phosphorylation by SRPK2 provokes its redistribution from the nuclear specke to nucleoplasm.
Top Pathways Spliceosome, Herpes simplex infection

SC35 (D-18) - sc-10250 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SC35 (D-18): sc-10250. Western blot analysis of SC35 expression in HeLa nuclear extract.

SC35 (E-16) - sc-10252 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SC35 (E-16): sc-10252. Western blot analysis of SC35 expression in HeLa nuclear extract.

SC35 (N-15) - sc-10249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SC35 (N-15): sc-10249. Western blot analysis of SC35 expression in A549 whole cell lysate.

SC35 (Y-16) - sc-10251 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SC35 (Y-16): sc-10251. Western blot analysis of SC35 expression in A549 whole cell lysate.

SC35/SRp46 (H-55) - sc-28720 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SC35/SRp46 (H-55): 28720. Western blot analysis of SC35/SRp46 expression in MES-SA/Dx5 whole cell lysate.

p-SC35 (SC-35) - sc-53518 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p-SC35 (SC-35): sc-53518. Immunoperoxidase staining of formalin fixed, paraffin-embedded human vulva/anal skin tissue showing nuclear speckle staining of epidermal cells.

Anti-SC35 antibody - ab28428 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SC35 antibody (ab28428)Lane 1 : recombinant SC35 protein Lane 2 : HeLa nuclear lysate

Anti-SC35 antibody - ab172262 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-SC35 antibody (ab172262) at 1/500 dilutionLane 1 : SC35 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker - ab11826 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone SC-35
ab11826 (1/1000) staining SC35 (phospho) in Human retinal pigment epithelial (RPE) cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.See Abreview

Anti-SRSF2 / SC35 Antibody (aa46-57) - LS-C10325 from LifeSpan Bioscience

Prices $325.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SRSF2 / SC35 Antibody - LS-C89240 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-SRSF2 / SC35 Antibody (aa83-113) - LS-C169026 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of SFRS2 Antibody in mouse stomach tissue lysates (35 ug/lane). SFRS2 (arrow) was detected using the purified antibody.

Anti-SRSF2 / SC35 Antibody (N-Terminus) - LS-C97287 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SFRS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-SRSF2 / SC35 Antibody (C-Terminus) IHC-plus™ - LS-B9760 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SRSF2 / SC35 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9760 concentration 10 ug/ml.

SC35 Antibody (SC-35) - NB100-1774 from Novus Biologicals

Prices $99.00, $279.00
Sizes 25 µl, 100 µl
Host Mouse
Clonality Monoclonal
Clone SC-35
Western Blot: SC35 Antibody (SC-35) [NB100-1774] - SC-35 recombinant protein (3 ug) was separated on SDS-PAGE and probed with Mouse Anti-Splicing Factor SC-35 Clone: SC-35. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:100; 2. Antibody dilution 1:250; 3. Antibody dilution 1:500; 4. Antibody dilution 1:1,000

SC35 Antibody - NBP1-79886 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SC35 Antibody [NBP1-79886] - Hela Cell Lysate at a concentration of 1ug/ml in 12%gel

SC35 Antibody - H00006427-B01 from Novus Biologicals

Host Mouse
Clonality Polyclonal
Western Blot: SC35 Antibody [H00006427-B01] - analysis of SFRS2 expression in transfected 293T cell line by SFRS2 MaxPab polyclonal antibody (B01). Lane 1: SFRS2 transfected lysate (19.80 KDa). Lane 2: Non-transfected lysate.

Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse - S4045 from Sigma-Aldrich

Prices $260.00, $340.00, $660.00
Sizes 100 µl, 200 µl, 500 µl
Host Mouse
Clonality Monoclonal
Clone SC-35
<B>Immunofluorescence</B><BR/>HS-68 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton<SUP>™</SUP> X-100. Fixed cells were stained with 1:2,000 Monoclonal Anti-Splicing Factor SC-35 Clone: SC-35 (<B>Cat. No. S4045</B>). The antibody was developed using Goat Anti-Mouse IgG, FITC conjugate.

Anti-SFRS2 antibody produced in rabbit - S2320 from Sigma-Aldrich

Prices $322.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ANTI-SFRS2(N-TERMINAL) antibody produced in rabbit - SAB1307090 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SFRS2 (N-term): <B>Cat. No. SAB1307090</B>: Western blot analysis of Anti-SFRS2 Antibody (N-term) in K562 cell line lysates (35 μg/lane).

Anti-SRSF2 antibody produced in rabbit - HPA049905 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-SRSF2: <B>Cat. No. HPA049905</B>: Immunohistochemical staining of human skin shows nuclear positivity.

SFRS2 antibody - C-terminal region (ARP61320_P050) - ARP61320_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SFRS2 Antibody - N-terminal region (OAAB01507) - OAAB01507 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

SFRS2 Antibody - middle region (OAAB01508) - OAAB01508 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

SFRS2-Antibody-Center - AP2800c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of SFRS2 Antibody (Center) (Cat. #AP2800c) in mouse stomach tissue lysates (35ug/lane). SFRS2 (arrow) was detected using the purified Pab.
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