| Gene Symbol | SRSF2 |
|---|---|
| Entrez Gene | 6427 |
| Alt Symbol | PR264, SC-35, SC35, SFRS2, SFRS2A, SRp30b |
| Species | Human |
| Gene Type | protein-coding |
| Description | serine/arginine-rich splicing factor 2 |
| Other Description | SR splicing factor 2|splicing component, 35 kDa|splicing factor SC35|splicing factor, arginine/serine-rich 2 |
| Swissprots | B4DN89 H0YG49 B3KWD5 Q01130 |
| Accessions | AAA60162 CAA53383 CAH25911 EAW89439 EAW89440 EAW89441 EAW89442 EAW89443 EAW89444 EAW89445 EAW89446 EAW89447 EAW89448 Q01130 AK092489 BAC03903 AK093626 BAC04206 AK097069 BAG53419 AK098209 BAG53593 AK123712 BAG53944 AK124792 BAG54097 AK126432 BAG54327 AK223252 BAD96972 AK297813 BAG60151 BC000339 AAH00339 BC001303 AAH01303 BC063570 BC066958 AAH66958 BC070086 AAH70086 BT007250 AAP35914 DA513281 DC361254 DQ891210 ABM82136 DQ894393 ABM85319 EB388696 M90104 AAA60306 X62447 CAA44307 XR_429913 XR_429914 XR_934515 XR_934516 NM_001195427 NP_001182356 NM_003016 NP_003007 NR_036608 |
| Function | Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. {ECO:0000269|PubMed:19592491, ECO:0000269|PubMed:21157427}. |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:21157427}. Nucleus, nucleoplasm {ECO:0000269|PubMed:21157427}. Nucleus speckle {ECO:0000269|PubMed:21157427}. Note=Phosphorylation by SRPK2 provokes its redistribution from the nuclear specke to nucleoplasm. |
| Top Pathways | Spliceosome, Herpes simplex infection |
SC35 CRISPR/Cas9 KO Plasmid (h) - sc-417676 from Santa Cruz Biotechnology
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SC35 HDR Plasmid (h) - sc-417676-HDR from Santa Cruz Biotechnology
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SC35 Double Nickase Plasmid (h) - sc-417676-NIC from Santa Cruz Biotechnology
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SC35 Double Nickase Plasmid (h2) - sc-417676-NIC-2 from Santa Cruz Biotechnology
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SC35 CRISPR Activation Plasmid (h) - sc-417676-ACT from Santa Cruz Biotechnology
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SC35 CRISPR Activation Plasmid (h2) - sc-417676-ACT-2 from Santa Cruz Biotechnology
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SC35 Lentiviral Activation Particles (h) - sc-417676-LAC from Santa Cruz Biotechnology
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SC35 Lentiviral Activation Particles (h2) - sc-417676-LAC-2 from Santa Cruz Biotechnology
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SC35 siRNA (h) - sc-38317 from Santa Cruz Biotechnology
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SC35 shRNA Plasmid (h) - sc-38317-SH from Santa Cruz Biotechnology
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SC35 shRNA (h) Lentiviral Particles - sc-38317-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU074471 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN20601 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND20601 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR12839 from Sigma-Aldrich
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SRSF2 siRNA (Human) - CRH4342 from Cohesion Biosciences
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SC35 RNAi - H00006427-R01 from Novus Biologicals
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SRSF2 - MBS8226690 from MyBioSource
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shRNA set against Human SRSF2 (NM_003016.4) - SHH418196 from Creative biogene
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Human SRSF2 siRNA - orb264844 from Biorbyt
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SRSF2 shRNA Plasmid (Human) - CHH4342 from Cohesion Biosciences
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SRSF2 miRNA 3'UTR clone - MiUTR4H-TG08541 from Creative biogene
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