VPS25

Gene Symbol VPS25
Entrez Gene 84313
Alt Symbol DERP9, EAP20, FAP20
Species Human
Gene Type protein-coding
Description vacuolar protein sorting 25 homolog (S. cerevisiae)
Other Description ELL-associated protein of 20 kDa|ESCRT-II complex subunit VPS25|dermal papilla-derived protein 9|vacuolar protein-sorting-associated protein 25
Swissprots B2R581 Q9BRG1
Accessions EAW60879 EAW60880 Q9BRG1 AB014763 BAB87804 AK312092 BAG35028 BC006282 AAH06282 BU630023 HQ448303 ADQ32783 NM_032353 NP_115729
Function Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. {ECO:0000269|PubMed:18723511}.
Subcellular Location Cytoplasm. Endosome membrane. Nucleus, nucleoplasm. Note=Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.
Tissue Specificity Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung. {ECO:0000269|PubMed:16889659}.
Top Pathways Endocytosis

VPS25 (B-4) - sc-271648 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPS25 (B-4): sc-271648. Western blot analysis of VPS25 expression in NIH/3T3 whole cell lysate (A) and HeLa nuclear extract (B).

VPS25 (D-6) - sc-271647 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPS25 (D-6): sc-271647. Western blot analysis of VPS25 expression in NIH/3T3 whole cell lysate (A) and HeLa nuclear extract (B).

VPS25 (FL-176) - sc-134749 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VPS25 (FL-176): sc-134749. Western blot analysis of VPS25 expression in K-562 (A) and NIH/3T3 (B) whole cell lysates.

VPS25 (N-16) - sc-49783 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

VPS25 (L-18) - sc-49782 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VPS25 (L-18): sc-49782. Western blot analysis of VPS25 expression in NIH/3T3 whole cell lysate.

Anti-VPS25 antibody - ab76210 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-VPS25 antibody - ab42500 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-VPS25 antibody (ab42500) at 0.5 µg/ml + Human Liver lysate (35ug protein in RIPA buffer).

Anti-VPS25 antibody - Aminoterminal end - ab56156 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-VPS25 antibody - C-terminal - ab156230 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-VPS25 antibody - C-terminal (ab156230) at 1/100 dilution + mouse kidney cell line lysates at 35 µg

Anti-VPS25 Antibody (aa116-144, AP) - LS-C242607 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS25 Antibody (aa116-144, FITC) - LS-C242609 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS25 Antibody (aa116-144, Biotin) - LS-C242608 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS25 Antibody (aa116-144, APC) - LS-C242610 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS25 Antibody (aa116-144, PE) - LS-C242613 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS25 Antibody (aa116-144, Azide-free, HRP) - LS-C247226 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS25 Antibody (aa116-144) - LS-C326291 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS25 Antibody (C-Terminus) - LS-C170339 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-VPS25 Antibody (aa20-29) - LS-C55671 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.5 ug/ml) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-VPS25 Antibody (aa116-144) - LS-C157034 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
VPS25 Antibody western blot of kidney cell line lysates (35 ug/lane). The VPS25 antibody detected the VPS25 protein (arrow).

Anti-VPS25 Antibody (N-Terminus) IHC-plus™ - LS-B2409 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-VPS25 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2409 concentration 5 ug/ml.

VPS25 Antibody - NB100-2468 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: VPS25 Antibody [NB100-2468] - (0.5ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

VPS25 Antibody (2E5-2B9) - H00084313-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E5-2B9
Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01] - MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Analysis of MGC10540 expression in Hela S3 NE.

VPS25 Antibody - NBP2-33824 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: VPS25 Antibody [NBP2-33824] - Staining of human epididymis shows cytoplasmic and membranous positivity in glandular cells.

VPS25 Antibody - NBP2-33641 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: VPS25 Antibody [NBP2-33641] - Staining of human cell line U-2 OS shows localization to centrosome.

VPS25 Antibody (4D6-2D4) - H00084313-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D6-2D4
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