Gene Symbol | VPS25 |
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Entrez Gene | 84313 |
Alt Symbol | DERP9, EAP20, FAP20 |
Species | Human |
Gene Type | protein-coding |
Description | vacuolar protein sorting 25 homolog (S. cerevisiae) |
Other Description | ELL-associated protein of 20 kDa|ESCRT-II complex subunit VPS25|dermal papilla-derived protein 9|vacuolar protein-sorting-associated protein 25 |
Swissprots | B2R581 Q9BRG1 |
Accessions | EAW60879 EAW60880 Q9BRG1 AB014763 BAB87804 AK312092 BAG35028 BC006282 AAH06282 BU630023 HQ448303 ADQ32783 NM_032353 NP_115729 |
Function | Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. {ECO:0000269|PubMed:18723511}. |
Subcellular Location | Cytoplasm. Endosome membrane. Nucleus, nucleoplasm. Note=Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6. |
Tissue Specificity | Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung. {ECO:0000269|PubMed:16889659}. |
Top Pathways | Endocytosis |
VPS25 CRISPR/Cas9 KO Plasmid (h) - sc-404109 from Santa Cruz Biotechnology
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VPS25 HDR Plasmid (h) - sc-404109-HDR from Santa Cruz Biotechnology
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VPS25 Double Nickase Plasmid (h) - sc-404109-NIC from Santa Cruz Biotechnology
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VPS25 Double Nickase Plasmid (h2) - sc-404109-NIC-2 from Santa Cruz Biotechnology
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VPS25 CRISPR Activation Plasmid (h) - sc-404109-ACT from Santa Cruz Biotechnology
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VPS25 CRISPR Activation Plasmid (h2) - sc-404109-ACT-2 from Santa Cruz Biotechnology
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VPS25 Lentiviral Activation Particles (h) - sc-404109-LAC from Santa Cruz Biotechnology
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VPS25 Lentiviral Activation Particles (h2) - sc-404109-LAC-2 from Santa Cruz Biotechnology
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VPS25 siRNA (h) - sc-61794 from Santa Cruz Biotechnology
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VPS25 shRNA Plasmid (h) - sc-61794-SH from Santa Cruz Biotechnology
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VPS25 shRNA (h) Lentiviral Particles - sc-61794-V from Santa Cruz Biotechnology
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VPS25 RNAi - H00084313-R01 from Novus Biologicals
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MISSION® esiRNA - EHU013451 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22712 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22712 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR04056 from Sigma-Aldrich
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VPS25 siRNA (Human) - CRJ3435 from Cohesion Biosciences
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VPS25 - MBS8237284 from MyBioSource
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shRNA set against Human VPS25 (NM_032353.2) - SHH444868 from Creative biogene
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shRNA set against Human VPS25(NM_032353.2) - SHL127416 from Creative biogene
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Human VPS25 siRNA - orb273936 from Biorbyt
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VPS25 shRNA Plasmid (Human) - CHJ3435 from Cohesion Biosciences
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VPS25 miRNA 3'UTR clone - MiUTR1H-11143 from Creative biogene
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